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Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients

OBJECTIVES: The natural history of HPV-related oropharyngeal squamous cell carcinoma (OPSCC) is still largely unknown. Since reports of second primary tumors (SPTs) in patients with HPV-related OPSCCs are increasing, a multifocal HPV infection, hinting a «virus-induced field effect», has been hypoth...

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Autores principales: Guarda, Vittoria, Schroeder, Lea, Pawlita, Michael, Ikenberg, Kristian, Rupp, Niels J., Jochum, Wolfram, Stoeckli, Sandro J., Holzinger, Dana, Broglie, Martina A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074725/
https://www.ncbi.nlm.nih.gov/pubmed/35530340
http://dx.doi.org/10.3389/fonc.2022.835814
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author Guarda, Vittoria
Schroeder, Lea
Pawlita, Michael
Ikenberg, Kristian
Rupp, Niels J.
Jochum, Wolfram
Stoeckli, Sandro J.
Holzinger, Dana
Broglie, Martina A.
author_facet Guarda, Vittoria
Schroeder, Lea
Pawlita, Michael
Ikenberg, Kristian
Rupp, Niels J.
Jochum, Wolfram
Stoeckli, Sandro J.
Holzinger, Dana
Broglie, Martina A.
author_sort Guarda, Vittoria
collection PubMed
description OBJECTIVES: The natural history of HPV-related oropharyngeal squamous cell carcinoma (OPSCC) is still largely unknown. Since reports of second primary tumors (SPTs) in patients with HPV-related OPSCCs are increasing, a multifocal HPV infection, hinting a «virus-induced field effect», has been hypothesized. This study aimed to investigate the HPV-prevalence in normal appearing oropharyngeal tissue in patients with OPSCCs. MATERIALS AND METHODS: 49 OPSCC patients undergoing panendoscopy were prospectively enrolled. Tumor specimens and biopsies of normal appearing oropharyngeal tissue adjacent to and distant from the index OPSCC underwent histopathological examination, p16(INK4A) immunohistochemical staining, HPV DNA and mRNA-detection. Patient characteristics and follow-up data on SPTs were obtained. RESULTS: 26 of 49 (53%) OPSCC were positive for HPV DNA and p16(INK4A). HPV mRNA was detected in 23 of 26 (88%) of these tumor samples. HPV DNA was detected in 36% adjacent mucosa and in 17% distant mucosa samples and only in patients with an HPV-related index OPSCC. HPV mRNA could not be detected in tumor-free distant and adjacent mucosa samples. No evidence of association between HPV detection in normal appearing mucosa and development of second primary tumors was found. CONCLUSIONS: HPV was detectable but not transcriptionally active in adjacent/distant tumor-free oropharyngeal tissue. This suggests that a multifocal HPV infection, hinting a «virus-induced fielcd cancerization», may not be pertaining to HPV-related OPSCC.
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spelling pubmed-90747252022-05-07 Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients Guarda, Vittoria Schroeder, Lea Pawlita, Michael Ikenberg, Kristian Rupp, Niels J. Jochum, Wolfram Stoeckli, Sandro J. Holzinger, Dana Broglie, Martina A. Front Oncol Oncology OBJECTIVES: The natural history of HPV-related oropharyngeal squamous cell carcinoma (OPSCC) is still largely unknown. Since reports of second primary tumors (SPTs) in patients with HPV-related OPSCCs are increasing, a multifocal HPV infection, hinting a «virus-induced field effect», has been hypothesized. This study aimed to investigate the HPV-prevalence in normal appearing oropharyngeal tissue in patients with OPSCCs. MATERIALS AND METHODS: 49 OPSCC patients undergoing panendoscopy were prospectively enrolled. Tumor specimens and biopsies of normal appearing oropharyngeal tissue adjacent to and distant from the index OPSCC underwent histopathological examination, p16(INK4A) immunohistochemical staining, HPV DNA and mRNA-detection. Patient characteristics and follow-up data on SPTs were obtained. RESULTS: 26 of 49 (53%) OPSCC were positive for HPV DNA and p16(INK4A). HPV mRNA was detected in 23 of 26 (88%) of these tumor samples. HPV DNA was detected in 36% adjacent mucosa and in 17% distant mucosa samples and only in patients with an HPV-related index OPSCC. HPV mRNA could not be detected in tumor-free distant and adjacent mucosa samples. No evidence of association between HPV detection in normal appearing mucosa and development of second primary tumors was found. CONCLUSIONS: HPV was detectable but not transcriptionally active in adjacent/distant tumor-free oropharyngeal tissue. This suggests that a multifocal HPV infection, hinting a «virus-induced fielcd cancerization», may not be pertaining to HPV-related OPSCC. Frontiers Media S.A. 2022-04-22 /pmc/articles/PMC9074725/ /pubmed/35530340 http://dx.doi.org/10.3389/fonc.2022.835814 Text en Copyright © 2022 Guarda, Schroeder, Pawlita, Ikenberg, Rupp, Jochum, Stoeckli, Holzinger and Broglie https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Guarda, Vittoria
Schroeder, Lea
Pawlita, Michael
Ikenberg, Kristian
Rupp, Niels J.
Jochum, Wolfram
Stoeckli, Sandro J.
Holzinger, Dana
Broglie, Martina A.
Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients
title Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients
title_full Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients
title_fullStr Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients
title_full_unstemmed Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients
title_short Prevalence of Transcriptionally Active HPV Infection in Tumor-Free Oropharyngeal Tissue of OPSCC-Patients
title_sort prevalence of transcriptionally active hpv infection in tumor-free oropharyngeal tissue of opscc-patients
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074725/
https://www.ncbi.nlm.nih.gov/pubmed/35530340
http://dx.doi.org/10.3389/fonc.2022.835814
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