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Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury

Spinal cord injury (SCI) leads to locomotor dysfunction. Locomotor rehabilitation promotes the recovery of stepping ability in lower mammals, but it has limited efficacy in humans with a severe SCI. To explain this discrepancy between different species, a nonhuman primate rehabilitation model with a...

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Autores principales: Kondo, Takahiro, Saito, Risa, Sato, Yuta, Sato, Kenta, Uchida, Akito, Yoshino-Saito, Kimika, Shinozaki, Munehisa, Tashiro, Syoichi, Nagoshi, Narihito, Nakamura, Masaya, Ushiba, Junichi, Okano, Hideyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074843/
https://www.ncbi.nlm.nih.gov/pubmed/35530175
http://dx.doi.org/10.3389/fncel.2022.858562
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author Kondo, Takahiro
Saito, Risa
Sato, Yuta
Sato, Kenta
Uchida, Akito
Yoshino-Saito, Kimika
Shinozaki, Munehisa
Tashiro, Syoichi
Nagoshi, Narihito
Nakamura, Masaya
Ushiba, Junichi
Okano, Hideyuki
author_facet Kondo, Takahiro
Saito, Risa
Sato, Yuta
Sato, Kenta
Uchida, Akito
Yoshino-Saito, Kimika
Shinozaki, Munehisa
Tashiro, Syoichi
Nagoshi, Narihito
Nakamura, Masaya
Ushiba, Junichi
Okano, Hideyuki
author_sort Kondo, Takahiro
collection PubMed
description Spinal cord injury (SCI) leads to locomotor dysfunction. Locomotor rehabilitation promotes the recovery of stepping ability in lower mammals, but it has limited efficacy in humans with a severe SCI. To explain this discrepancy between different species, a nonhuman primate rehabilitation model with a severe SCI would be useful. In this study, we developed a rehabilitation model of paraplegia caused by a severe traumatic SCI in a nonhuman primate, common marmoset (Callithrix jacchus). The locomotor rating scale for marmosets was developed to accurately assess the recovery of locomotor functions in marmosets. All animals showed flaccid paralysis of the hindlimb after a thoracic contusive SCI, but the trained group showed significant locomotor recovery. Kinematic analysis revealed significantly improved hindlimb stepping patterns in trained marmosets. Furthermore, intracortical microstimulation (ICMS) of the motor cortex evoked the hindlimb muscles in the trained group, suggesting the reconnection between supraspinal input and the lumbosacral network. Because rehabilitation may be combined with regenerative interventions such as medicine or cell therapy, this primate model can be used as a preclinical test of therapies that can be used in human clinical trials.
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spelling pubmed-90748432022-05-07 Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury Kondo, Takahiro Saito, Risa Sato, Yuta Sato, Kenta Uchida, Akito Yoshino-Saito, Kimika Shinozaki, Munehisa Tashiro, Syoichi Nagoshi, Narihito Nakamura, Masaya Ushiba, Junichi Okano, Hideyuki Front Cell Neurosci Cellular Neuroscience Spinal cord injury (SCI) leads to locomotor dysfunction. Locomotor rehabilitation promotes the recovery of stepping ability in lower mammals, but it has limited efficacy in humans with a severe SCI. To explain this discrepancy between different species, a nonhuman primate rehabilitation model with a severe SCI would be useful. In this study, we developed a rehabilitation model of paraplegia caused by a severe traumatic SCI in a nonhuman primate, common marmoset (Callithrix jacchus). The locomotor rating scale for marmosets was developed to accurately assess the recovery of locomotor functions in marmosets. All animals showed flaccid paralysis of the hindlimb after a thoracic contusive SCI, but the trained group showed significant locomotor recovery. Kinematic analysis revealed significantly improved hindlimb stepping patterns in trained marmosets. Furthermore, intracortical microstimulation (ICMS) of the motor cortex evoked the hindlimb muscles in the trained group, suggesting the reconnection between supraspinal input and the lumbosacral network. Because rehabilitation may be combined with regenerative interventions such as medicine or cell therapy, this primate model can be used as a preclinical test of therapies that can be used in human clinical trials. Frontiers Media S.A. 2022-04-22 /pmc/articles/PMC9074843/ /pubmed/35530175 http://dx.doi.org/10.3389/fncel.2022.858562 Text en Copyright © 2022 Kondo, Saito, Sato, Sato, Uchida, Yoshino-Saito, Shinozaki, Tashiro, Nagoshi, Nakamura, Ushiba and Okano. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular Neuroscience
Kondo, Takahiro
Saito, Risa
Sato, Yuta
Sato, Kenta
Uchida, Akito
Yoshino-Saito, Kimika
Shinozaki, Munehisa
Tashiro, Syoichi
Nagoshi, Narihito
Nakamura, Masaya
Ushiba, Junichi
Okano, Hideyuki
Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury
title Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury
title_full Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury
title_fullStr Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury
title_full_unstemmed Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury
title_short Treadmill Training for Common Marmoset to Strengthen Corticospinal Connections After Thoracic Contusion Spinal Cord Injury
title_sort treadmill training for common marmoset to strengthen corticospinal connections after thoracic contusion spinal cord injury
topic Cellular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074843/
https://www.ncbi.nlm.nih.gov/pubmed/35530175
http://dx.doi.org/10.3389/fncel.2022.858562
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