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miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis

MicroRNAs play an important role in myogenic differentiation, they bind to target genes and regulate muscle formation. We previously found that miR-9-5p, which is related to bone formation, was increased over time during the process of myogenic differentiation. However, the mechanism by which miR-9-...

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Autores principales: Dong, Liying, Wang, Meng, Gao, Xiaolei, Zheng, Xuan, Zhang, Yixin, Sun, Liangjie, Zhao, Na, Ding, Chong, Ma, Zeyun, Wang, Yixiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074878/
https://www.ncbi.nlm.nih.gov/pubmed/35529491
http://dx.doi.org/10.7717/peerj.13360
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author Dong, Liying
Wang, Meng
Gao, Xiaolei
Zheng, Xuan
Zhang, Yixin
Sun, Liangjie
Zhao, Na
Ding, Chong
Ma, Zeyun
Wang, Yixiang
author_facet Dong, Liying
Wang, Meng
Gao, Xiaolei
Zheng, Xuan
Zhang, Yixin
Sun, Liangjie
Zhao, Na
Ding, Chong
Ma, Zeyun
Wang, Yixiang
author_sort Dong, Liying
collection PubMed
description MicroRNAs play an important role in myogenic differentiation, they bind to target genes and regulate muscle formation. We previously found that miR-9-5p, which is related to bone formation, was increased over time during the process of myogenic differentiation. However, the mechanism by which miR-9-5p regulates myogenic differentiation remains largely unknown. In the present study, we first examined myotube formation and miR-9-5p, myogenesis-related genes including Dlx3, Myod1, Mef2c, Desmin, MyoG and Myf5 expression under myogenic induction. Then, we detected the expression of myogenic transcription factors after overexpression or knockdown of miR-9-5p or Dlx3 in the mouse premyoblast cell line C2C12 by qPCR, western blot and myotube formation under myogenic induction. A luciferase assay was performed to confirm the regulatory relationships between not only miR-9-5p and Dlx3 but also Dlx3 and its downstream gene, Myf5, which is an essential transcription factor of myogenic differentiation. The results showed that miR-9-5p promoted myogenic differentiation by increasing myogenic transcription factor expression and promoting myotube formation, but Dlx3 exerted the opposite effect. Moreover, the luciferase assay showed that miR-9-5p bound to the 3’UTR of Dlx3 and downregulated Dlx3 expression. Dlx3 in turn suppressed Myf5 expression by binding to the Myf5 promoter, ultimately inhibiting the process of myogenic differentiation. In conclusion, the miR-9-5p/Dlx3/Myf5 axis is a novel pathway for the regulation of myogenic differentiation, and can be a potential target to treat the diseases related to muscle dysfunction.
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spelling pubmed-90748782022-05-07 miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis Dong, Liying Wang, Meng Gao, Xiaolei Zheng, Xuan Zhang, Yixin Sun, Liangjie Zhao, Na Ding, Chong Ma, Zeyun Wang, Yixiang PeerJ Biochemistry MicroRNAs play an important role in myogenic differentiation, they bind to target genes and regulate muscle formation. We previously found that miR-9-5p, which is related to bone formation, was increased over time during the process of myogenic differentiation. However, the mechanism by which miR-9-5p regulates myogenic differentiation remains largely unknown. In the present study, we first examined myotube formation and miR-9-5p, myogenesis-related genes including Dlx3, Myod1, Mef2c, Desmin, MyoG and Myf5 expression under myogenic induction. Then, we detected the expression of myogenic transcription factors after overexpression or knockdown of miR-9-5p or Dlx3 in the mouse premyoblast cell line C2C12 by qPCR, western blot and myotube formation under myogenic induction. A luciferase assay was performed to confirm the regulatory relationships between not only miR-9-5p and Dlx3 but also Dlx3 and its downstream gene, Myf5, which is an essential transcription factor of myogenic differentiation. The results showed that miR-9-5p promoted myogenic differentiation by increasing myogenic transcription factor expression and promoting myotube formation, but Dlx3 exerted the opposite effect. Moreover, the luciferase assay showed that miR-9-5p bound to the 3’UTR of Dlx3 and downregulated Dlx3 expression. Dlx3 in turn suppressed Myf5 expression by binding to the Myf5 promoter, ultimately inhibiting the process of myogenic differentiation. In conclusion, the miR-9-5p/Dlx3/Myf5 axis is a novel pathway for the regulation of myogenic differentiation, and can be a potential target to treat the diseases related to muscle dysfunction. PeerJ Inc. 2022-05-03 /pmc/articles/PMC9074878/ /pubmed/35529491 http://dx.doi.org/10.7717/peerj.13360 Text en ©2022 Dong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Biochemistry
Dong, Liying
Wang, Meng
Gao, Xiaolei
Zheng, Xuan
Zhang, Yixin
Sun, Liangjie
Zhao, Na
Ding, Chong
Ma, Zeyun
Wang, Yixiang
miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis
title miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis
title_full miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis
title_fullStr miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis
title_full_unstemmed miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis
title_short miR-9-5p promotes myogenic differentiation via the Dlx3/Myf5 axis
title_sort mir-9-5p promotes myogenic differentiation via the dlx3/myf5 axis
topic Biochemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074878/
https://www.ncbi.nlm.nih.gov/pubmed/35529491
http://dx.doi.org/10.7717/peerj.13360
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