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An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration

Cell–hydrogel constructs are frequently used as injectable platforms for irregular cartilage regeneration. However, cell–hydrogel constructs have obvious disadvantages, such as long culture times, high probability of infection, and poor cartilage formation capacity, significantly limiting their clin...

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Autores principales: Xu, Wei, Wang, Tao, Wang, Yahui, Wu, Xiaodi, Chen, Yujie, Song, Daiying, Ci, Zheng, Cao, Yilin, Hua, Yujie, Zhou, Guangdong, Liu, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074996/
https://www.ncbi.nlm.nih.gov/pubmed/35528206
http://dx.doi.org/10.3389/fbioe.2022.884036
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author Xu, Wei
Wang, Tao
Wang, Yahui
Wu, Xiaodi
Chen, Yujie
Song, Daiying
Ci, Zheng
Cao, Yilin
Hua, Yujie
Zhou, Guangdong
Liu, Yu
author_facet Xu, Wei
Wang, Tao
Wang, Yahui
Wu, Xiaodi
Chen, Yujie
Song, Daiying
Ci, Zheng
Cao, Yilin
Hua, Yujie
Zhou, Guangdong
Liu, Yu
author_sort Xu, Wei
collection PubMed
description Cell–hydrogel constructs are frequently used as injectable platforms for irregular cartilage regeneration. However, cell–hydrogel constructs have obvious disadvantages, such as long culture times, high probability of infection, and poor cartilage formation capacity, significantly limiting their clinical translation. In this study, we aimed to develop a novel injectable platform comprising engineered cartilage gel (ECG) and gelatin methacrylate (GelMA) to improve cartilage regeneration. We first prepared an ECG by cutting the in vitro engineered cartilage sheet into pieces. The chondrocytes and ECG were evenly encapsulated into GelMA to form Cell-GelMA and ECG-GelMA constructs. The ECG-GelMA construct exhibited preferred gel characteristics and superior biocompatibility compared with the Cell-GelMA construct counterpart. After subcutaneous implantation in nude mice and goat, both gross views and histological evaluations showed that the ECG-GelMA construct achieved more homogenous, stable, and mature cartilage regeneration than the Cell-GelMA construct. Immunological evaluations showed that ECG-GelMA had a mitigatory immunologic reaction than the Cell-GelMA construct. Overall, the results suggest that the ECG-GelMA is a promising injectable platform for cartilage regeneration that may advance clinical translation.
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spelling pubmed-90749962022-05-07 An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration Xu, Wei Wang, Tao Wang, Yahui Wu, Xiaodi Chen, Yujie Song, Daiying Ci, Zheng Cao, Yilin Hua, Yujie Zhou, Guangdong Liu, Yu Front Bioeng Biotechnol Bioengineering and Biotechnology Cell–hydrogel constructs are frequently used as injectable platforms for irregular cartilage regeneration. However, cell–hydrogel constructs have obvious disadvantages, such as long culture times, high probability of infection, and poor cartilage formation capacity, significantly limiting their clinical translation. In this study, we aimed to develop a novel injectable platform comprising engineered cartilage gel (ECG) and gelatin methacrylate (GelMA) to improve cartilage regeneration. We first prepared an ECG by cutting the in vitro engineered cartilage sheet into pieces. The chondrocytes and ECG were evenly encapsulated into GelMA to form Cell-GelMA and ECG-GelMA constructs. The ECG-GelMA construct exhibited preferred gel characteristics and superior biocompatibility compared with the Cell-GelMA construct counterpart. After subcutaneous implantation in nude mice and goat, both gross views and histological evaluations showed that the ECG-GelMA construct achieved more homogenous, stable, and mature cartilage regeneration than the Cell-GelMA construct. Immunological evaluations showed that ECG-GelMA had a mitigatory immunologic reaction than the Cell-GelMA construct. Overall, the results suggest that the ECG-GelMA is a promising injectable platform for cartilage regeneration that may advance clinical translation. Frontiers Media S.A. 2022-04-14 /pmc/articles/PMC9074996/ /pubmed/35528206 http://dx.doi.org/10.3389/fbioe.2022.884036 Text en Copyright © 2022 Xu, Wang, Wang, Wu, Chen, Song, Ci, Cao, Hua, Zhou and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Xu, Wei
Wang, Tao
Wang, Yahui
Wu, Xiaodi
Chen, Yujie
Song, Daiying
Ci, Zheng
Cao, Yilin
Hua, Yujie
Zhou, Guangdong
Liu, Yu
An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration
title An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration
title_full An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration
title_fullStr An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration
title_full_unstemmed An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration
title_short An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration
title_sort injectable platform of engineered cartilage gel and gelatin methacrylate to promote cartilage regeneration
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9074996/
https://www.ncbi.nlm.nih.gov/pubmed/35528206
http://dx.doi.org/10.3389/fbioe.2022.884036
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