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Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia
INTRODUCTION: Toxoplasma gondii is a parasite with great zoonotic potential. It can infect a broad range of warm-blooded hosts (including livestock) and causes significant losses in the industry. In humans, it has been described as a pathogen in immunosuppressed people, it affects the fetus developm...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Instituto Nacional de Salud
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075112/ https://www.ncbi.nlm.nih.gov/pubmed/35471176 http://dx.doi.org/10.7705/biomedica.6251 |
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author | Medina, Juan David Osorio, Laura Alejandra Zabala, Daniel Wagner de Almeida Vitor, Ricardo Gómez, Jorge Enrique Carranza, Julio César Vallejo, Gustavo Adolfo |
author_facet | Medina, Juan David Osorio, Laura Alejandra Zabala, Daniel Wagner de Almeida Vitor, Ricardo Gómez, Jorge Enrique Carranza, Julio César Vallejo, Gustavo Adolfo |
author_sort | Medina, Juan David |
collection | PubMed |
description | INTRODUCTION: Toxoplasma gondii is a parasite with great zoonotic potential. It can infect a broad range of warm-blooded hosts (including livestock) and causes significant losses in the industry. In humans, it has been described as a pathogen in immunosuppressed people, it affects the fetus development in congenital infections, and is associated with various behavioral disorders in healthy people. Humans can acquire T. gondii by consuming undercooked, contaminated meat. OBJECTIVE: To determine T. gondii positivity (currently unknown) in meat for human consumption (i.e., beef, chicken, and pork) in the city of Ibague, Colombia. MATERIALS AND METHODS: We used conventional nested PCR and the T. gondii B1 gene sequence as amplification target. We collected samples of meat (N=186) sold in the urban area of Ibagué (62 beef, 62 chicken, and 62 pork samples) and determined the T. gondii positivity percentage for each type of meat. RESULTS: The study found an average of 18.8% positivity for all meat samples, pork having the highest percentage (22.5%; 14/62), followed by beef (19.3%; 12/62) and chicken (14.5%; 9/62). The best-amplified products were sequenced by macrogen and aligned with the B1 gene sequences in GenBank, thereby confirming their identity. CONCLUSIONS: This is the first study of T. gondii prevalence in meat for human consumption carried out in the city of Ibagué and the department of Tolima. All three types of meat sampled represent a risk for local human infection. |
format | Online Article Text |
id | pubmed-9075112 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Instituto Nacional de Salud |
record_format | MEDLINE/PubMed |
spelling | pubmed-90751122022-05-09 Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia Medina, Juan David Osorio, Laura Alejandra Zabala, Daniel Wagner de Almeida Vitor, Ricardo Gómez, Jorge Enrique Carranza, Julio César Vallejo, Gustavo Adolfo Biomedica Artículo Original INTRODUCTION: Toxoplasma gondii is a parasite with great zoonotic potential. It can infect a broad range of warm-blooded hosts (including livestock) and causes significant losses in the industry. In humans, it has been described as a pathogen in immunosuppressed people, it affects the fetus development in congenital infections, and is associated with various behavioral disorders in healthy people. Humans can acquire T. gondii by consuming undercooked, contaminated meat. OBJECTIVE: To determine T. gondii positivity (currently unknown) in meat for human consumption (i.e., beef, chicken, and pork) in the city of Ibague, Colombia. MATERIALS AND METHODS: We used conventional nested PCR and the T. gondii B1 gene sequence as amplification target. We collected samples of meat (N=186) sold in the urban area of Ibagué (62 beef, 62 chicken, and 62 pork samples) and determined the T. gondii positivity percentage for each type of meat. RESULTS: The study found an average of 18.8% positivity for all meat samples, pork having the highest percentage (22.5%; 14/62), followed by beef (19.3%; 12/62) and chicken (14.5%; 9/62). The best-amplified products were sequenced by macrogen and aligned with the B1 gene sequences in GenBank, thereby confirming their identity. CONCLUSIONS: This is the first study of T. gondii prevalence in meat for human consumption carried out in the city of Ibagué and the department of Tolima. All three types of meat sampled represent a risk for local human infection. Instituto Nacional de Salud 2022-03-01 /pmc/articles/PMC9075112/ /pubmed/35471176 http://dx.doi.org/10.7705/biomedica.6251 Text en https://creativecommons.org/licenses/by/4.0/Este es un artículo publicado en acceso abierto bajo una licencia Creative Commons |
spellingShingle | Artículo Original Medina, Juan David Osorio, Laura Alejandra Zabala, Daniel Wagner de Almeida Vitor, Ricardo Gómez, Jorge Enrique Carranza, Julio César Vallejo, Gustavo Adolfo Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia |
title | Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia |
title_full | Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia |
title_fullStr | Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia |
title_full_unstemmed | Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia |
title_short | Detección molecular de Toxoplasma gondii en carnes para consumo humano en Ibagué, Colombia |
title_sort | detección molecular de toxoplasma gondii en carnes para consumo humano en ibagué, colombia |
topic | Artículo Original |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075112/ https://www.ncbi.nlm.nih.gov/pubmed/35471176 http://dx.doi.org/10.7705/biomedica.6251 |
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