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An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling

Ketamine is a commonly abused drug due to its stimulant, dissociative and hallucinogenic effects. An overdose of ketamine has been found to cause a variety of side effects. Therefore, the identification and quantification of ketamine are of significant importance for clinical purposes and drug seizi...

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Detalles Bibliográficos
Autores principales: Chen, Hong, Zou, Yun, Jiang, Xue, Cao, Fangqi, Liu, Wenbin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075121/
https://www.ncbi.nlm.nih.gov/pubmed/35539066
http://dx.doi.org/10.1039/c9ra06139h
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author Chen, Hong
Zou, Yun
Jiang, Xue
Cao, Fangqi
Liu, Wenbin
author_facet Chen, Hong
Zou, Yun
Jiang, Xue
Cao, Fangqi
Liu, Wenbin
author_sort Chen, Hong
collection PubMed
description Ketamine is a commonly abused drug due to its stimulant, dissociative and hallucinogenic effects. An overdose of ketamine has been found to cause a variety of side effects. Therefore, the identification and quantification of ketamine are of significant importance for clinical purposes and drug seizing. However, conventional methods for ketamine detection possess some disadvantages such as sophisticated procedures, expensive instruments and low sensitivity. Herein, we develop a novel fluorescent nanoprobe for ultrasensitive ketamine detection with signal amplification based on Adenosine Triphosphate (ATP)-fueled target recycling and FRET (fluorescence resonance energy transfer) occurring between the FAM (Fluorescein, tagged with Y-shape DNA) and AuNPs. Based on the combination of FRET and signals circle amplification, the gold nanospheres functionalized with Y-motif DNA (Y@AuNPs) nanoprobe was utilized for effective ketamine detection with the limit of detection (LOD) down to 3 pg mL(−1), which was lower than previously reported. Furthermore, the high sensitivity of Y@AuNPs facilitated quantitative analysis in biological media and practical samples.
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spelling pubmed-90751212022-05-09 An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling Chen, Hong Zou, Yun Jiang, Xue Cao, Fangqi Liu, Wenbin RSC Adv Chemistry Ketamine is a commonly abused drug due to its stimulant, dissociative and hallucinogenic effects. An overdose of ketamine has been found to cause a variety of side effects. Therefore, the identification and quantification of ketamine are of significant importance for clinical purposes and drug seizing. However, conventional methods for ketamine detection possess some disadvantages such as sophisticated procedures, expensive instruments and low sensitivity. Herein, we develop a novel fluorescent nanoprobe for ultrasensitive ketamine detection with signal amplification based on Adenosine Triphosphate (ATP)-fueled target recycling and FRET (fluorescence resonance energy transfer) occurring between the FAM (Fluorescein, tagged with Y-shape DNA) and AuNPs. Based on the combination of FRET and signals circle amplification, the gold nanospheres functionalized with Y-motif DNA (Y@AuNPs) nanoprobe was utilized for effective ketamine detection with the limit of detection (LOD) down to 3 pg mL(−1), which was lower than previously reported. Furthermore, the high sensitivity of Y@AuNPs facilitated quantitative analysis in biological media and practical samples. The Royal Society of Chemistry 2019-11-12 /pmc/articles/PMC9075121/ /pubmed/35539066 http://dx.doi.org/10.1039/c9ra06139h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Chen, Hong
Zou, Yun
Jiang, Xue
Cao, Fangqi
Liu, Wenbin
An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
title An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
title_full An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
title_fullStr An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
title_full_unstemmed An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
title_short An enzyme-free FRET nanoprobe for ultrasensitive ketamine detection based on ATP-fueled target recycling
title_sort enzyme-free fret nanoprobe for ultrasensitive ketamine detection based on atp-fueled target recycling
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075121/
https://www.ncbi.nlm.nih.gov/pubmed/35539066
http://dx.doi.org/10.1039/c9ra06139h
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