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Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3))
The ovicidal and larvicidal effect of a full aqueous extract (FE) and two phases: an aqueous (Aq-Ph) and an ethyl acetate (EtOAc-Ph) from Ruta chalepensis (Rc) stems and leaves against Haemonchus contortus (Hc) were assessed. The egg hatching inhibition (EHI) assay and larval mortality (LM) test wer...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075876/ https://www.ncbi.nlm.nih.gov/pubmed/36756302 http://dx.doi.org/10.2478/helm-2022-0001 |
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author | Jasso-Díaz, G. Torres-Hernández, G. Zamilpa, A. Becerril-Pérez, C.M. Ramírez-Bribiesca, J. E. Hernández-Mendo, O. Sánchez-Arroyo, H. Olmedo-Juárez, A. González-Cortazar, M. Mendoza-De Gives, P. |
author_facet | Jasso-Díaz, G. Torres-Hernández, G. Zamilpa, A. Becerril-Pérez, C.M. Ramírez-Bribiesca, J. E. Hernández-Mendo, O. Sánchez-Arroyo, H. Olmedo-Juárez, A. González-Cortazar, M. Mendoza-De Gives, P. |
author_sort | Jasso-Díaz, G. |
collection | PubMed |
description | The ovicidal and larvicidal effect of a full aqueous extract (FE) and two phases: an aqueous (Aq-Ph) and an ethyl acetate (EtOAc-Ph) from Ruta chalepensis (Rc) stems and leaves against Haemonchus contortus (Hc) were assessed. The egg hatching inhibition (EHI) assay and larval mortality (LM) test were performed by triplicate in 96-well micro-titration plates (n=4 wells). The FE against Hc eggs and larvae was assessed at 1.25, 2.5, 5, 10 and 20 mg/mL; and 30, 60, 90, 120,150 and 200 mg/ mL, respectively. The ovicidal effect of Aq-F and EtOAc-F was assessed at 1.25, 2.5, 5, 10 and 20 mg/mL. Plates were incubated at 28 °C for 48 (ovicidal assay) and 72 h (larvicidal assay). The EHI results were considered based on the mean number of eggs hatching failure after 48 h exposure. The LM was recorded after 72 h exposure to the Rc phases and expressed as mortality percentage. The Rc FE caused 96 and 100% EHI at 10 and 20 mg/mL, respectively; meanwhile, 74% LM was recorded at 200 mg/mL (p<0.05). The Aq-Ph showed 78.5% EHI at 2.5 mg/mL. Likewise, the EtOAc-Ph caused 100% EHI in almost all concentrations. Eighteen compounds including alkaloids, cumarins, triterpens, flavonoids, tannins, saponins and sterols were identified by GC-MS analysis. The results indicate that the aqueous extract from Rc possesses bioactive compounds with in vitro nematocidal activity against Hc; mainly in the EtOAc-Ph. Further studies should be performed to elucidate those compounds searching for alternative methods of control of the sheep haemonchosis. |
format | Online Article Text |
id | pubmed-9075876 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-90758762023-02-07 Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3)) Jasso-Díaz, G. Torres-Hernández, G. Zamilpa, A. Becerril-Pérez, C.M. Ramírez-Bribiesca, J. E. Hernández-Mendo, O. Sánchez-Arroyo, H. Olmedo-Juárez, A. González-Cortazar, M. Mendoza-De Gives, P. Helminthologia Research Article The ovicidal and larvicidal effect of a full aqueous extract (FE) and two phases: an aqueous (Aq-Ph) and an ethyl acetate (EtOAc-Ph) from Ruta chalepensis (Rc) stems and leaves against Haemonchus contortus (Hc) were assessed. The egg hatching inhibition (EHI) assay and larval mortality (LM) test were performed by triplicate in 96-well micro-titration plates (n=4 wells). The FE against Hc eggs and larvae was assessed at 1.25, 2.5, 5, 10 and 20 mg/mL; and 30, 60, 90, 120,150 and 200 mg/ mL, respectively. The ovicidal effect of Aq-F and EtOAc-F was assessed at 1.25, 2.5, 5, 10 and 20 mg/mL. Plates were incubated at 28 °C for 48 (ovicidal assay) and 72 h (larvicidal assay). The EHI results were considered based on the mean number of eggs hatching failure after 48 h exposure. The LM was recorded after 72 h exposure to the Rc phases and expressed as mortality percentage. The Rc FE caused 96 and 100% EHI at 10 and 20 mg/mL, respectively; meanwhile, 74% LM was recorded at 200 mg/mL (p<0.05). The Aq-Ph showed 78.5% EHI at 2.5 mg/mL. Likewise, the EtOAc-Ph caused 100% EHI in almost all concentrations. Eighteen compounds including alkaloids, cumarins, triterpens, flavonoids, tannins, saponins and sterols were identified by GC-MS analysis. The results indicate that the aqueous extract from Rc possesses bioactive compounds with in vitro nematocidal activity against Hc; mainly in the EtOAc-Ph. Further studies should be performed to elucidate those compounds searching for alternative methods of control of the sheep haemonchosis. Sciendo 2022-05-03 /pmc/articles/PMC9075876/ /pubmed/36756302 http://dx.doi.org/10.2478/helm-2022-0001 Text en © 2022 G. Jasso-Díaz, G. Torres-Hernández, A. Zamilpa, C.M. Becerril-Pérez, J. E. Ramírez-Bribiesca, O. Hernández-Mendo, H. Sánchez-Arroyo, A. Olmedo-Juárez, M. González-Cortazar, P. Mendoza-De Gives, published by Sciendo https://creativecommons.org/licenses/by-nc-nd/4.0/This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License. |
spellingShingle | Research Article Jasso-Díaz, G. Torres-Hernández, G. Zamilpa, A. Becerril-Pérez, C.M. Ramírez-Bribiesca, J. E. Hernández-Mendo, O. Sánchez-Arroyo, H. Olmedo-Juárez, A. González-Cortazar, M. Mendoza-De Gives, P. Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3)) |
title | Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3)) |
title_full | Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3)) |
title_fullStr | Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3)) |
title_full_unstemmed | Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3)) |
title_short | Ruta Chalepensis Full Extract and Organic Phases Exhibit Nematocidal Activity Against Haemonchus Contortus Eggs and Infective Larvae (L(3)) |
title_sort | ruta chalepensis full extract and organic phases exhibit nematocidal activity against haemonchus contortus eggs and infective larvae (l(3)) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075876/ https://www.ncbi.nlm.nih.gov/pubmed/36756302 http://dx.doi.org/10.2478/helm-2022-0001 |
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