Cargando…

High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry

Metabolomics methods can be used to explore the effect mechanisms underlying treatments with traditional medicine. Lung cancer (LC) causes the highest morbidity and mortality among tumors disease, and has become a serious public health problem. Chuanxiong (CX) is a dried rhizome of Ligusticum Chuanx...

Descripción completa

Detalles Bibliográficos
Autores principales: Luo, Wen, Zhang, Jia-Wen, Zhang, Li-Juan, Zhang, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075942/
https://www.ncbi.nlm.nih.gov/pubmed/35540684
http://dx.doi.org/10.1039/c9ra06267j
_version_ 1784701798823493632
author Luo, Wen
Zhang, Jia-Wen
Zhang, Li-Juan
Zhang, Wei
author_facet Luo, Wen
Zhang, Jia-Wen
Zhang, Li-Juan
Zhang, Wei
author_sort Luo, Wen
collection PubMed
description Metabolomics methods can be used to explore the effect mechanisms underlying treatments with traditional medicine. Lung cancer (LC) causes the highest morbidity and mortality among tumors disease, and has become a serious public health problem. Chuanxiong (CX) is a dried rhizome of Ligusticum Chuanxiong Hort., often used in traditional Chinese medicine and has been widely used in the treatment for tumors. However, the pharmacological effect of CX on the metabolism process of LC mice is still unclear. This study used high-throughput untargeted metabolomics aims to discover biomarkers and metabolic pathways of LC as a potential target to provide insight into the pharmacological action and effective mechanism of CX against LC. The precise structural identification of the LC biomarker has been established using ultra performance liquid chromatography (UPLC) combined with quadrupole-time-of-flight-mass spectrometry (Q-TOF-MS) technology. UPLC-Q-TOF-MS and chemometrics methods were used to analyze the blood metabolism of LC model mice, and revealed the intervention effect of CX on LC model mice and potential therapeutic targets. The results showed that the metabolic profile clustering among the groups was obvious, and 31 potential biomarkers were finally locked, involving 7 related metabolic pathways. After treatment with CX, we found that 22 kinds of biomarkers were recalled to the main metabolic pathway which are associated with lipid metabolism. This study provides an effective biomarker reference for early clinical diagnosis of LC, and also provides a foundation for the expansion of new drugs for CX treatment of LC.
format Online
Article
Text
id pubmed-9075942
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90759422022-05-09 High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry Luo, Wen Zhang, Jia-Wen Zhang, Li-Juan Zhang, Wei RSC Adv Chemistry Metabolomics methods can be used to explore the effect mechanisms underlying treatments with traditional medicine. Lung cancer (LC) causes the highest morbidity and mortality among tumors disease, and has become a serious public health problem. Chuanxiong (CX) is a dried rhizome of Ligusticum Chuanxiong Hort., often used in traditional Chinese medicine and has been widely used in the treatment for tumors. However, the pharmacological effect of CX on the metabolism process of LC mice is still unclear. This study used high-throughput untargeted metabolomics aims to discover biomarkers and metabolic pathways of LC as a potential target to provide insight into the pharmacological action and effective mechanism of CX against LC. The precise structural identification of the LC biomarker has been established using ultra performance liquid chromatography (UPLC) combined with quadrupole-time-of-flight-mass spectrometry (Q-TOF-MS) technology. UPLC-Q-TOF-MS and chemometrics methods were used to analyze the blood metabolism of LC model mice, and revealed the intervention effect of CX on LC model mice and potential therapeutic targets. The results showed that the metabolic profile clustering among the groups was obvious, and 31 potential biomarkers were finally locked, involving 7 related metabolic pathways. After treatment with CX, we found that 22 kinds of biomarkers were recalled to the main metabolic pathway which are associated with lipid metabolism. This study provides an effective biomarker reference for early clinical diagnosis of LC, and also provides a foundation for the expansion of new drugs for CX treatment of LC. The Royal Society of Chemistry 2019-11-28 /pmc/articles/PMC9075942/ /pubmed/35540684 http://dx.doi.org/10.1039/c9ra06267j Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Luo, Wen
Zhang, Jia-Wen
Zhang, Li-Juan
Zhang, Wei
High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry
title High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry
title_full High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry
title_fullStr High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry
title_full_unstemmed High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry
title_short High-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry
title_sort high-throughput untargeted metabolomics and chemometrics reveals pharmacological action and molecular mechanism of chuanxiong by ultra performance liquid chromatography combined with quadrupole-time-of-flight-mass spectrometry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075942/
https://www.ncbi.nlm.nih.gov/pubmed/35540684
http://dx.doi.org/10.1039/c9ra06267j
work_keys_str_mv AT luowen highthroughputuntargetedmetabolomicsandchemometricsrevealspharmacologicalactionandmolecularmechanismofchuanxiongbyultraperformanceliquidchromatographycombinedwithquadrupoletimeofflightmassspectrometry
AT zhangjiawen highthroughputuntargetedmetabolomicsandchemometricsrevealspharmacologicalactionandmolecularmechanismofchuanxiongbyultraperformanceliquidchromatographycombinedwithquadrupoletimeofflightmassspectrometry
AT zhanglijuan highthroughputuntargetedmetabolomicsandchemometricsrevealspharmacologicalactionandmolecularmechanismofchuanxiongbyultraperformanceliquidchromatographycombinedwithquadrupoletimeofflightmassspectrometry
AT zhangwei highthroughputuntargetedmetabolomicsandchemometricsrevealspharmacologicalactionandmolecularmechanismofchuanxiongbyultraperformanceliquidchromatographycombinedwithquadrupoletimeofflightmassspectrometry