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Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice

The cerebellum is hypothesized to represent timing information important for organizing salient motor events during periodically performed discontinuous movements. To provide functional evidence validating this idea, we measured and manipulated Purkinje cell (PC) activity in the lateral cerebellum o...

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Autores principales: Gaffield, Michael A, Sauerbrei, Britton A, Christie, Jason M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075950/
https://www.ncbi.nlm.nih.gov/pubmed/35451957
http://dx.doi.org/10.7554/eLife.71464
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author Gaffield, Michael A
Sauerbrei, Britton A
Christie, Jason M
author_facet Gaffield, Michael A
Sauerbrei, Britton A
Christie, Jason M
author_sort Gaffield, Michael A
collection PubMed
description The cerebellum is hypothesized to represent timing information important for organizing salient motor events during periodically performed discontinuous movements. To provide functional evidence validating this idea, we measured and manipulated Purkinje cell (PC) activity in the lateral cerebellum of mice trained to volitionally perform periodic bouts of licking for regularly allocated water rewards. Overall, PC simple spiking modulated during task performance, mapping phasic tongue protrusions and retractions, as well as ramping prior to both lick-bout initiation and termination, two important motor events delimiting movement cycles. The ramping onset occurred earlier for the initiation of uncued exploratory licking that anticipated water availability relative to licking that was reactive to water allocation, suggesting that the cerebellum is engaged differently depending on the movement context. In a subpopulation of PCs, climbing-fiber-evoked responses also increased during lick-bout initiation, but not termination, highlighting differences in how cerebellar input pathways represent task-related information. Optogenetic perturbation of PC activity disrupted the behavior by degrading lick-bout rhythmicity in addition to initiating and terminating licking bouts confirming a causative role in movement organization. Together, these results substantiate that the cerebellum contributes to the initiation and timing of repeated motor actions.
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spelling pubmed-90759502022-05-07 Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice Gaffield, Michael A Sauerbrei, Britton A Christie, Jason M eLife Neuroscience The cerebellum is hypothesized to represent timing information important for organizing salient motor events during periodically performed discontinuous movements. To provide functional evidence validating this idea, we measured and manipulated Purkinje cell (PC) activity in the lateral cerebellum of mice trained to volitionally perform periodic bouts of licking for regularly allocated water rewards. Overall, PC simple spiking modulated during task performance, mapping phasic tongue protrusions and retractions, as well as ramping prior to both lick-bout initiation and termination, two important motor events delimiting movement cycles. The ramping onset occurred earlier for the initiation of uncued exploratory licking that anticipated water availability relative to licking that was reactive to water allocation, suggesting that the cerebellum is engaged differently depending on the movement context. In a subpopulation of PCs, climbing-fiber-evoked responses also increased during lick-bout initiation, but not termination, highlighting differences in how cerebellar input pathways represent task-related information. Optogenetic perturbation of PC activity disrupted the behavior by degrading lick-bout rhythmicity in addition to initiating and terminating licking bouts confirming a causative role in movement organization. Together, these results substantiate that the cerebellum contributes to the initiation and timing of repeated motor actions. eLife Sciences Publications, Ltd 2022-04-22 /pmc/articles/PMC9075950/ /pubmed/35451957 http://dx.doi.org/10.7554/eLife.71464 Text en © 2022, Gaffield et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Neuroscience
Gaffield, Michael A
Sauerbrei, Britton A
Christie, Jason M
Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice
title Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice
title_full Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice
title_fullStr Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice
title_full_unstemmed Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice
title_short Cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice
title_sort cerebellum encodes and influences the initiation, performance, and termination of discontinuous movements in mice
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075950/
https://www.ncbi.nlm.nih.gov/pubmed/35451957
http://dx.doi.org/10.7554/eLife.71464
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