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Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa

Biofilm formation is one of most important causes leading to persistent infections. Exopolysaccharides are usually a main component of biofilm matrix. Genes encoding glycosyl hydrolases are often found in gene clusters that are involved in the exopolysaccharide synthesis. It remains elusive about th...

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Autores principales: Zhang, Jingchao, Wu, Huijun, Wang, Di, Wang, Lanxin, Cui, Yifan, Zhang, Chenxi, Zhao, Kun, Ma, Luyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075953/
https://www.ncbi.nlm.nih.gov/pubmed/35438634
http://dx.doi.org/10.7554/eLife.72778
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author Zhang, Jingchao
Wu, Huijun
Wang, Di
Wang, Lanxin
Cui, Yifan
Zhang, Chenxi
Zhao, Kun
Ma, Luyan
author_facet Zhang, Jingchao
Wu, Huijun
Wang, Di
Wang, Lanxin
Cui, Yifan
Zhang, Chenxi
Zhao, Kun
Ma, Luyan
author_sort Zhang, Jingchao
collection PubMed
description Biofilm formation is one of most important causes leading to persistent infections. Exopolysaccharides are usually a main component of biofilm matrix. Genes encoding glycosyl hydrolases are often found in gene clusters that are involved in the exopolysaccharide synthesis. It remains elusive about the functions of intracellular glycosyl hydrolase and why a polysaccharide synthesis gene cluster requires a glycosyl hydrolase-encoding gene. Here, we systematically studied the physiologically relevant role of intracellular PslG, a glycosyl hydrolase whose encoding gene is co-transcribed with 15 psl genes, which is responsible for the synthesis of exopolysaccharide PSL, a key biofilm matrix polysaccharide in opportunistic pathogen Pseudomonas aeruginosa. We showed that lack of PslG or its hydrolytic activity in this opportunistic pathogen enhances the signaling function of PSL, changes the relative level of cyclic-di-GMP within daughter cells during cell division and shapes the localization of PSL on bacterial periphery, thus results in long chains of bacterial cells, fast-forming biofilm microcolonies. Our results reveal the important roles of intracellular PslG on the cell fate and biofilm development.
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spelling pubmed-90759532022-05-07 Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa Zhang, Jingchao Wu, Huijun Wang, Di Wang, Lanxin Cui, Yifan Zhang, Chenxi Zhao, Kun Ma, Luyan eLife Microbiology and Infectious Disease Biofilm formation is one of most important causes leading to persistent infections. Exopolysaccharides are usually a main component of biofilm matrix. Genes encoding glycosyl hydrolases are often found in gene clusters that are involved in the exopolysaccharide synthesis. It remains elusive about the functions of intracellular glycosyl hydrolase and why a polysaccharide synthesis gene cluster requires a glycosyl hydrolase-encoding gene. Here, we systematically studied the physiologically relevant role of intracellular PslG, a glycosyl hydrolase whose encoding gene is co-transcribed with 15 psl genes, which is responsible for the synthesis of exopolysaccharide PSL, a key biofilm matrix polysaccharide in opportunistic pathogen Pseudomonas aeruginosa. We showed that lack of PslG or its hydrolytic activity in this opportunistic pathogen enhances the signaling function of PSL, changes the relative level of cyclic-di-GMP within daughter cells during cell division and shapes the localization of PSL on bacterial periphery, thus results in long chains of bacterial cells, fast-forming biofilm microcolonies. Our results reveal the important roles of intracellular PslG on the cell fate and biofilm development. eLife Sciences Publications, Ltd 2022-04-19 /pmc/articles/PMC9075953/ /pubmed/35438634 http://dx.doi.org/10.7554/eLife.72778 Text en © 2022, Zhang, Wu, Wang et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Microbiology and Infectious Disease
Zhang, Jingchao
Wu, Huijun
Wang, Di
Wang, Lanxin
Cui, Yifan
Zhang, Chenxi
Zhao, Kun
Ma, Luyan
Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa
title Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa
title_full Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa
title_fullStr Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa
title_full_unstemmed Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa
title_short Intracellular glycosyl hydrolase PslG shapes bacterial cell fate, signaling, and the biofilm development of Pseudomonas aeruginosa
title_sort intracellular glycosyl hydrolase pslg shapes bacterial cell fate, signaling, and the biofilm development of pseudomonas aeruginosa
topic Microbiology and Infectious Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9075953/
https://www.ncbi.nlm.nih.gov/pubmed/35438634
http://dx.doi.org/10.7554/eLife.72778
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