Cargando…

Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1

Chronic hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC). Certain studies have revealed that microRNAs play crucial roles in HBV-related HCC. The aim of this study was to investigate the effects of microRNA-1271 (miR-1271) on HBV replication, cell proliferation...

Descripción completa

Detalles Bibliográficos
Autores principales: Tang, Fei, Wang, Fengmei, Lv, Hongmin, Xiang, Huiling, Liu, Yi, Liu, Ping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076187/
https://www.ncbi.nlm.nih.gov/pubmed/35541395
http://dx.doi.org/10.1039/c9ra08248d
_version_ 1784701858798895104
author Tang, Fei
Wang, Fengmei
Lv, Hongmin
Xiang, Huiling
Liu, Yi
Liu, Ping
author_facet Tang, Fei
Wang, Fengmei
Lv, Hongmin
Xiang, Huiling
Liu, Yi
Liu, Ping
author_sort Tang, Fei
collection PubMed
description Chronic hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC). Certain studies have revealed that microRNAs play crucial roles in HBV-related HCC. The aim of this study was to investigate the effects of microRNA-1271 (miR-1271) on HBV replication, cell proliferation and apoptosis in HBV-related HCC. The expression of HBV DNA and miR-1271 was detected by quantitative real time-polymerase chain reaction (qRT-PCR). The mRNA and protein levels of SIRT1 were detected by qRT-PCR and western blot analysis, respectively. HBV replication was assessed by the expression of HBV DNA and the levels of HBsAg and HBeAg. Cell proliferation was assessed by cell counting kit-8 (CCK-8) and 5-bromo-2-deoxyuidine (BrdU) assay, and apoptosis was evaluated by flow cytometry assay, enzyme-linked immunosorbent assay (ELISA) and the activity of caspase-3. The relationship between miR-1271 and SIRT1 was predicated by online software and confirmed by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) and pull-down assay. We first found that the expression of miR-1271 was downregulated and SIRT1 was upregulated in both HBV-related HCC tissues and cells. Overexpression of miR-1271 inhibited HBV replication and cell proliferation whilst promoting apoptosis in HBV-related HCC cells. Subsequently, SIRT1 was identified as a target of miR-1271. Moreover, overexpression of SIRT1 reversed the effects of miR-1271 overexpression on HBV replication, cell proliferation and apoptosis in HBV-related HCC cells. In conclusion, our study demonstrated that miR-1271 inhibited HBV replication and proliferation and promoted apoptosis of HBV-related HCC cells via targeting SIRT1, which might contribute to the diagnosis and therapy of HBV-related HCC.
format Online
Article
Text
id pubmed-9076187
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90761872022-05-09 Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1 Tang, Fei Wang, Fengmei Lv, Hongmin Xiang, Huiling Liu, Yi Liu, Ping RSC Adv Chemistry Chronic hepatitis B virus (HBV) infection is a leading cause of hepatocellular carcinoma (HCC). Certain studies have revealed that microRNAs play crucial roles in HBV-related HCC. The aim of this study was to investigate the effects of microRNA-1271 (miR-1271) on HBV replication, cell proliferation and apoptosis in HBV-related HCC. The expression of HBV DNA and miR-1271 was detected by quantitative real time-polymerase chain reaction (qRT-PCR). The mRNA and protein levels of SIRT1 were detected by qRT-PCR and western blot analysis, respectively. HBV replication was assessed by the expression of HBV DNA and the levels of HBsAg and HBeAg. Cell proliferation was assessed by cell counting kit-8 (CCK-8) and 5-bromo-2-deoxyuidine (BrdU) assay, and apoptosis was evaluated by flow cytometry assay, enzyme-linked immunosorbent assay (ELISA) and the activity of caspase-3. The relationship between miR-1271 and SIRT1 was predicated by online software and confirmed by dual-luciferase reporter assay, RNA immunoprecipitation (RIP) and pull-down assay. We first found that the expression of miR-1271 was downregulated and SIRT1 was upregulated in both HBV-related HCC tissues and cells. Overexpression of miR-1271 inhibited HBV replication and cell proliferation whilst promoting apoptosis in HBV-related HCC cells. Subsequently, SIRT1 was identified as a target of miR-1271. Moreover, overexpression of SIRT1 reversed the effects of miR-1271 overexpression on HBV replication, cell proliferation and apoptosis in HBV-related HCC cells. In conclusion, our study demonstrated that miR-1271 inhibited HBV replication and proliferation and promoted apoptosis of HBV-related HCC cells via targeting SIRT1, which might contribute to the diagnosis and therapy of HBV-related HCC. The Royal Society of Chemistry 2019-12-02 /pmc/articles/PMC9076187/ /pubmed/35541395 http://dx.doi.org/10.1039/c9ra08248d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Tang, Fei
Wang, Fengmei
Lv, Hongmin
Xiang, Huiling
Liu, Yi
Liu, Ping
Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1
title Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1
title_full Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1
title_fullStr Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1
title_full_unstemmed Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1
title_short Retracted Article: MicroRNA-1271 modulates hepatitis B virus replication, cell proliferation and apoptosis in hepatitis B virus-related hepatocellular carcinoma by targeting SIRT1
title_sort retracted article: microrna-1271 modulates hepatitis b virus replication, cell proliferation and apoptosis in hepatitis b virus-related hepatocellular carcinoma by targeting sirt1
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076187/
https://www.ncbi.nlm.nih.gov/pubmed/35541395
http://dx.doi.org/10.1039/c9ra08248d
work_keys_str_mv AT tangfei retractedarticlemicrorna1271modulateshepatitisbvirusreplicationcellproliferationandapoptosisinhepatitisbvirusrelatedhepatocellularcarcinomabytargetingsirt1
AT wangfengmei retractedarticlemicrorna1271modulateshepatitisbvirusreplicationcellproliferationandapoptosisinhepatitisbvirusrelatedhepatocellularcarcinomabytargetingsirt1
AT lvhongmin retractedarticlemicrorna1271modulateshepatitisbvirusreplicationcellproliferationandapoptosisinhepatitisbvirusrelatedhepatocellularcarcinomabytargetingsirt1
AT xianghuiling retractedarticlemicrorna1271modulateshepatitisbvirusreplicationcellproliferationandapoptosisinhepatitisbvirusrelatedhepatocellularcarcinomabytargetingsirt1
AT liuyi retractedarticlemicrorna1271modulateshepatitisbvirusreplicationcellproliferationandapoptosisinhepatitisbvirusrelatedhepatocellularcarcinomabytargetingsirt1
AT liuping retractedarticlemicrorna1271modulateshepatitisbvirusreplicationcellproliferationandapoptosisinhepatitisbvirusrelatedhepatocellularcarcinomabytargetingsirt1