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Monitoring glycosidase activity for clustered sugar substrates, a study on β-glucuronidase

Determination of glycosidase hydrolysis kinetics for a monovalent sugar substrate is relatively straightforward and classically achieved by monitoring the fluorescence signal released from the sugar-conjugated probe after enzymatic hydrolysis. Naturally occuring sugar epitopes are, however, often cl...

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Detalles Bibliográficos
Autores principales: Brissonnet, Yoan, Compain, Guillaume, Renoux, Brigitte, Krammer, Eva-Maria, Daligault, Franck, Deniaud, David, Papot, Sébastien, Gouin, Sébastien G.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076263/
https://www.ncbi.nlm.nih.gov/pubmed/35542663
http://dx.doi.org/10.1039/c9ra08847d

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