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Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L.

Vacuole compartmentalization plays an important role in the storage of heavy metals in hyperaccumulators. Is the vacuolar compartmentation a simple shielding process or a dynamic process that continuously consumes cell sap resources? How does glutathione affect the process of vacuolar compartmentali...

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Autores principales: Li, Zhishuai, Guan, Wenjie, Yang, Lan, Yang, Yan, Yu, Hongyan, Zou, Luyi, Teng, Yue
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076330/
https://www.ncbi.nlm.nih.gov/pubmed/35528170
http://dx.doi.org/10.1155/2022/4359645
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author Li, Zhishuai
Guan, Wenjie
Yang, Lan
Yang, Yan
Yu, Hongyan
Zou, Luyi
Teng, Yue
author_facet Li, Zhishuai
Guan, Wenjie
Yang, Lan
Yang, Yan
Yu, Hongyan
Zou, Luyi
Teng, Yue
author_sort Li, Zhishuai
collection PubMed
description Vacuole compartmentalization plays an important role in the storage of heavy metals in hyperaccumulators. Is the vacuolar compartmentation a simple shielding process or a dynamic process that continuously consumes cell sap resources? How does glutathione affect the process of vacuolar compartmentalization? These unknown questions are very important to understand the mechanism of vacuole compartmentalization and can provide a guide for the design of hyperaccumulator plants by genetic engineering. Therefore, this study explored the enzyme activities, total cadmium, Cd(2+), glutathione, oxidized glutathione, and reactive oxygen species contents in protoplasts and vacuoles of leaf cells in Solanum nigrum L. through subcellular separation. The results showed that vacuolar compartmentalization was a dynamic process that actively induced the related substances produced by cell sap to enter the vacuole for detoxification. When regulating the decreased glutathione content with buthionine sulfoximine, the total cadmium and combined cadmium in protoplasm decreased significantly, but the vacuole still maintained a high proportion of cadmium content and stable ROS content, which indicated that various external resources were preferentially used to maintain cadmium storage and homeostasis in vacuole rather than outside vacuole. These findings could guide the use of genetic engineering to design hyperaccumulator plants.
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spelling pubmed-90763302022-05-07 Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L. Li, Zhishuai Guan, Wenjie Yang, Lan Yang, Yan Yu, Hongyan Zou, Luyi Teng, Yue Biomed Res Int Research Article Vacuole compartmentalization plays an important role in the storage of heavy metals in hyperaccumulators. Is the vacuolar compartmentation a simple shielding process or a dynamic process that continuously consumes cell sap resources? How does glutathione affect the process of vacuolar compartmentalization? These unknown questions are very important to understand the mechanism of vacuole compartmentalization and can provide a guide for the design of hyperaccumulator plants by genetic engineering. Therefore, this study explored the enzyme activities, total cadmium, Cd(2+), glutathione, oxidized glutathione, and reactive oxygen species contents in protoplasts and vacuoles of leaf cells in Solanum nigrum L. through subcellular separation. The results showed that vacuolar compartmentalization was a dynamic process that actively induced the related substances produced by cell sap to enter the vacuole for detoxification. When regulating the decreased glutathione content with buthionine sulfoximine, the total cadmium and combined cadmium in protoplasm decreased significantly, but the vacuole still maintained a high proportion of cadmium content and stable ROS content, which indicated that various external resources were preferentially used to maintain cadmium storage and homeostasis in vacuole rather than outside vacuole. These findings could guide the use of genetic engineering to design hyperaccumulator plants. Hindawi 2022-04-29 /pmc/articles/PMC9076330/ /pubmed/35528170 http://dx.doi.org/10.1155/2022/4359645 Text en Copyright © 2022 Zhishuai Li et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Li, Zhishuai
Guan, Wenjie
Yang, Lan
Yang, Yan
Yu, Hongyan
Zou, Luyi
Teng, Yue
Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L.
title Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L.
title_full Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L.
title_fullStr Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L.
title_full_unstemmed Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L.
title_short Insight into the Vacuolar Compartmentalization Process and the Effect Glutathione Regulation to This Process in the Hyperaccumulator Plant Solanum nigrum L.
title_sort insight into the vacuolar compartmentalization process and the effect glutathione regulation to this process in the hyperaccumulator plant solanum nigrum l.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076330/
https://www.ncbi.nlm.nih.gov/pubmed/35528170
http://dx.doi.org/10.1155/2022/4359645
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