Cargando…

Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2

Flavin-mediated extracellular electron transfer was studied in two Gram-positive bacteria: Bacillus cereus strain DIF1 and Rhodococcus ruber strain DIF2. The electrochemical activities of these strains were confirmed using amperometric I–t curves and cyclic voltammetry (CV). Spent anodes with biofil...

Descripción completa

Detalles Bibliográficos
Autores principales: Tian, Tian, Fan, Xiaoyang, Feng, Man, Su, Lin, Zhang, Wen, Chi, Huimei, Fu, Degang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076428/
https://www.ncbi.nlm.nih.gov/pubmed/35540069
http://dx.doi.org/10.1039/c9ra08045g
_version_ 1784701919913050112
author Tian, Tian
Fan, Xiaoyang
Feng, Man
Su, Lin
Zhang, Wen
Chi, Huimei
Fu, Degang
author_facet Tian, Tian
Fan, Xiaoyang
Feng, Man
Su, Lin
Zhang, Wen
Chi, Huimei
Fu, Degang
author_sort Tian, Tian
collection PubMed
description Flavin-mediated extracellular electron transfer was studied in two Gram-positive bacteria: Bacillus cereus strain DIF1 and Rhodococcus ruber strain DIF2. The electrochemical activities of these strains were confirmed using amperometric I–t curves and cyclic voltammetry (CV). Spent anodes with biofilms in fresh anolytes showed no redox peaks, while new anodes in the spent broth showed relative redox peaks using CV measurements, indicating the presence of a redox electron mediator secreted by bacteria. Adding riboflavins (RF) and flavin mononucleotide (FMN) improved the electron transfer of the microbial fuel cells inoculated with the two strains. The redox peaks indicated that flavins existed in the anolyte, and HPLC analysis showed that RF and FMN were secreted by the two bacterial strains. The concentration of RF increased until the bacteria grew to the log phase in microbial fuel cells. The concentration of RF decreased and that of FMN increased after the log phase. The two strains secreted FMN only in the microbial fuel cell. These results confirmed that the electrochemical activity mediated by flavins and FMN is essential in the extracellular electron transfer process in the strains DIF1 and DIF2.
format Online
Article
Text
id pubmed-9076428
institution National Center for Biotechnology Information
language English
publishDate 2019
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90764282022-05-09 Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 Tian, Tian Fan, Xiaoyang Feng, Man Su, Lin Zhang, Wen Chi, Huimei Fu, Degang RSC Adv Chemistry Flavin-mediated extracellular electron transfer was studied in two Gram-positive bacteria: Bacillus cereus strain DIF1 and Rhodococcus ruber strain DIF2. The electrochemical activities of these strains were confirmed using amperometric I–t curves and cyclic voltammetry (CV). Spent anodes with biofilms in fresh anolytes showed no redox peaks, while new anodes in the spent broth showed relative redox peaks using CV measurements, indicating the presence of a redox electron mediator secreted by bacteria. Adding riboflavins (RF) and flavin mononucleotide (FMN) improved the electron transfer of the microbial fuel cells inoculated with the two strains. The redox peaks indicated that flavins existed in the anolyte, and HPLC analysis showed that RF and FMN were secreted by the two bacterial strains. The concentration of RF increased until the bacteria grew to the log phase in microbial fuel cells. The concentration of RF decreased and that of FMN increased after the log phase. The two strains secreted FMN only in the microbial fuel cell. These results confirmed that the electrochemical activity mediated by flavins and FMN is essential in the extracellular electron transfer process in the strains DIF1 and DIF2. The Royal Society of Chemistry 2019-12-11 /pmc/articles/PMC9076428/ /pubmed/35540069 http://dx.doi.org/10.1039/c9ra08045g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Tian, Tian
Fan, Xiaoyang
Feng, Man
Su, Lin
Zhang, Wen
Chi, Huimei
Fu, Degang
Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
title Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
title_full Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
title_fullStr Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
title_full_unstemmed Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
title_short Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
title_sort flavin-mediated extracellular electron transfer in gram-positive bacteria bacillus cereus dif1 and rhodococcus ruber dif2
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076428/
https://www.ncbi.nlm.nih.gov/pubmed/35540069
http://dx.doi.org/10.1039/c9ra08045g
work_keys_str_mv AT tiantian flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2
AT fanxiaoyang flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2
AT fengman flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2
AT sulin flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2
AT zhangwen flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2
AT chihuimei flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2
AT fudegang flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2