Cargando…
Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2
Flavin-mediated extracellular electron transfer was studied in two Gram-positive bacteria: Bacillus cereus strain DIF1 and Rhodococcus ruber strain DIF2. The electrochemical activities of these strains were confirmed using amperometric I–t curves and cyclic voltammetry (CV). Spent anodes with biofil...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076428/ https://www.ncbi.nlm.nih.gov/pubmed/35540069 http://dx.doi.org/10.1039/c9ra08045g |
_version_ | 1784701919913050112 |
---|---|
author | Tian, Tian Fan, Xiaoyang Feng, Man Su, Lin Zhang, Wen Chi, Huimei Fu, Degang |
author_facet | Tian, Tian Fan, Xiaoyang Feng, Man Su, Lin Zhang, Wen Chi, Huimei Fu, Degang |
author_sort | Tian, Tian |
collection | PubMed |
description | Flavin-mediated extracellular electron transfer was studied in two Gram-positive bacteria: Bacillus cereus strain DIF1 and Rhodococcus ruber strain DIF2. The electrochemical activities of these strains were confirmed using amperometric I–t curves and cyclic voltammetry (CV). Spent anodes with biofilms in fresh anolytes showed no redox peaks, while new anodes in the spent broth showed relative redox peaks using CV measurements, indicating the presence of a redox electron mediator secreted by bacteria. Adding riboflavins (RF) and flavin mononucleotide (FMN) improved the electron transfer of the microbial fuel cells inoculated with the two strains. The redox peaks indicated that flavins existed in the anolyte, and HPLC analysis showed that RF and FMN were secreted by the two bacterial strains. The concentration of RF increased until the bacteria grew to the log phase in microbial fuel cells. The concentration of RF decreased and that of FMN increased after the log phase. The two strains secreted FMN only in the microbial fuel cell. These results confirmed that the electrochemical activity mediated by flavins and FMN is essential in the extracellular electron transfer process in the strains DIF1 and DIF2. |
format | Online Article Text |
id | pubmed-9076428 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90764282022-05-09 Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 Tian, Tian Fan, Xiaoyang Feng, Man Su, Lin Zhang, Wen Chi, Huimei Fu, Degang RSC Adv Chemistry Flavin-mediated extracellular electron transfer was studied in two Gram-positive bacteria: Bacillus cereus strain DIF1 and Rhodococcus ruber strain DIF2. The electrochemical activities of these strains were confirmed using amperometric I–t curves and cyclic voltammetry (CV). Spent anodes with biofilms in fresh anolytes showed no redox peaks, while new anodes in the spent broth showed relative redox peaks using CV measurements, indicating the presence of a redox electron mediator secreted by bacteria. Adding riboflavins (RF) and flavin mononucleotide (FMN) improved the electron transfer of the microbial fuel cells inoculated with the two strains. The redox peaks indicated that flavins existed in the anolyte, and HPLC analysis showed that RF and FMN were secreted by the two bacterial strains. The concentration of RF increased until the bacteria grew to the log phase in microbial fuel cells. The concentration of RF decreased and that of FMN increased after the log phase. The two strains secreted FMN only in the microbial fuel cell. These results confirmed that the electrochemical activity mediated by flavins and FMN is essential in the extracellular electron transfer process in the strains DIF1 and DIF2. The Royal Society of Chemistry 2019-12-11 /pmc/articles/PMC9076428/ /pubmed/35540069 http://dx.doi.org/10.1039/c9ra08045g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Tian, Tian Fan, Xiaoyang Feng, Man Su, Lin Zhang, Wen Chi, Huimei Fu, Degang Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 |
title | Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 |
title_full | Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 |
title_fullStr | Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 |
title_full_unstemmed | Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 |
title_short | Flavin-mediated extracellular electron transfer in Gram-positive bacteria Bacillus cereus DIF1 and Rhodococcus ruber DIF2 |
title_sort | flavin-mediated extracellular electron transfer in gram-positive bacteria bacillus cereus dif1 and rhodococcus ruber dif2 |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076428/ https://www.ncbi.nlm.nih.gov/pubmed/35540069 http://dx.doi.org/10.1039/c9ra08045g |
work_keys_str_mv | AT tiantian flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2 AT fanxiaoyang flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2 AT fengman flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2 AT sulin flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2 AT zhangwen flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2 AT chihuimei flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2 AT fudegang flavinmediatedextracellularelectrontransferingrampositivebacteriabacilluscereusdif1andrhodococcusruberdif2 |