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Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations

In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specif...

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Autores principales: Duchamp, Margaux, Dahoun, Thamani, Vaillier, Clarisse, Arnaud, Marion, Bobisse, Sara, Coukos, George, Harari, Alexandre, Renaud, Philippe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076435/
https://www.ncbi.nlm.nih.gov/pubmed/35540074
http://dx.doi.org/10.1039/c9ra09504g
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author Duchamp, Margaux
Dahoun, Thamani
Vaillier, Clarisse
Arnaud, Marion
Bobisse, Sara
Coukos, George
Harari, Alexandre
Renaud, Philippe
author_facet Duchamp, Margaux
Dahoun, Thamani
Vaillier, Clarisse
Arnaud, Marion
Bobisse, Sara
Coukos, George
Harari, Alexandre
Renaud, Philippe
author_sort Duchamp, Margaux
collection PubMed
description In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specific locations, then relied on hydrodynamic filtering principles, the flowing cells from the second cell population are placed in contact with the trapped ones, through a roll-over mechanism. The rolling cells interact with the serially trapped cells one after the other. The proposed microfluidic phenomenon was characterized with beads. We have shown the validity of our method by detecting the capacity of olfactory receptors to induce adhesion of cell doublets overexpressing these receptors. We report here the first controlled on-flow single cell resolution cell–cell interaction assay in a microfluidic device for future application in cell–cell interactions-based cell library screenings.
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spelling pubmed-90764352022-05-09 Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations Duchamp, Margaux Dahoun, Thamani Vaillier, Clarisse Arnaud, Marion Bobisse, Sara Coukos, George Harari, Alexandre Renaud, Philippe RSC Adv Chemistry In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specific locations, then relied on hydrodynamic filtering principles, the flowing cells from the second cell population are placed in contact with the trapped ones, through a roll-over mechanism. The rolling cells interact with the serially trapped cells one after the other. The proposed microfluidic phenomenon was characterized with beads. We have shown the validity of our method by detecting the capacity of olfactory receptors to induce adhesion of cell doublets overexpressing these receptors. We report here the first controlled on-flow single cell resolution cell–cell interaction assay in a microfluidic device for future application in cell–cell interactions-based cell library screenings. The Royal Society of Chemistry 2019-12-13 /pmc/articles/PMC9076435/ /pubmed/35540074 http://dx.doi.org/10.1039/c9ra09504g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Duchamp, Margaux
Dahoun, Thamani
Vaillier, Clarisse
Arnaud, Marion
Bobisse, Sara
Coukos, George
Harari, Alexandre
Renaud, Philippe
Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
title Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
title_full Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
title_fullStr Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
title_full_unstemmed Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
title_short Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
title_sort microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076435/
https://www.ncbi.nlm.nih.gov/pubmed/35540074
http://dx.doi.org/10.1039/c9ra09504g
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