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Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations
In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specif...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2019
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076435/ https://www.ncbi.nlm.nih.gov/pubmed/35540074 http://dx.doi.org/10.1039/c9ra09504g |
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author | Duchamp, Margaux Dahoun, Thamani Vaillier, Clarisse Arnaud, Marion Bobisse, Sara Coukos, George Harari, Alexandre Renaud, Philippe |
author_facet | Duchamp, Margaux Dahoun, Thamani Vaillier, Clarisse Arnaud, Marion Bobisse, Sara Coukos, George Harari, Alexandre Renaud, Philippe |
author_sort | Duchamp, Margaux |
collection | PubMed |
description | In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specific locations, then relied on hydrodynamic filtering principles, the flowing cells from the second cell population are placed in contact with the trapped ones, through a roll-over mechanism. The rolling cells interact with the serially trapped cells one after the other. The proposed microfluidic phenomenon was characterized with beads. We have shown the validity of our method by detecting the capacity of olfactory receptors to induce adhesion of cell doublets overexpressing these receptors. We report here the first controlled on-flow single cell resolution cell–cell interaction assay in a microfluidic device for future application in cell–cell interactions-based cell library screenings. |
format | Online Article Text |
id | pubmed-9076435 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2019 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90764352022-05-09 Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations Duchamp, Margaux Dahoun, Thamani Vaillier, Clarisse Arnaud, Marion Bobisse, Sara Coukos, George Harari, Alexandre Renaud, Philippe RSC Adv Chemistry In this study we present a novel microfluidic hydrodynamic trapping device to probe the cell–cell interaction between all cell samples of two distinct populations. We have exploited an hydrodynamic trapping method using microfluidics to immobilize a batch of cells from the first population at specific locations, then relied on hydrodynamic filtering principles, the flowing cells from the second cell population are placed in contact with the trapped ones, through a roll-over mechanism. The rolling cells interact with the serially trapped cells one after the other. The proposed microfluidic phenomenon was characterized with beads. We have shown the validity of our method by detecting the capacity of olfactory receptors to induce adhesion of cell doublets overexpressing these receptors. We report here the first controlled on-flow single cell resolution cell–cell interaction assay in a microfluidic device for future application in cell–cell interactions-based cell library screenings. The Royal Society of Chemistry 2019-12-13 /pmc/articles/PMC9076435/ /pubmed/35540074 http://dx.doi.org/10.1039/c9ra09504g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Duchamp, Margaux Dahoun, Thamani Vaillier, Clarisse Arnaud, Marion Bobisse, Sara Coukos, George Harari, Alexandre Renaud, Philippe Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations |
title | Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations |
title_full | Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations |
title_fullStr | Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations |
title_full_unstemmed | Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations |
title_short | Microfluidic device performing on flow study of serial cell–cell interactions of two cell populations |
title_sort | microfluidic device performing on flow study of serial cell–cell interactions of two cell populations |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076435/ https://www.ncbi.nlm.nih.gov/pubmed/35540074 http://dx.doi.org/10.1039/c9ra09504g |
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