Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging

Near-infrared (NIR) fluorescent probes are widely employed in biological detection because of their lower damage to biological samples, low background interference, and high signal-to-noise ratio. Herein, a highly water-soluble NIR probe (NIRHA) based on a hemicyanine skeleton and bearing an acrylat...

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Detalles Bibliográficos
Autores principales: Qi, Shaolong, Zhu, Lubao, Wang, Xinyu, Du, Jianshi, Yang, Qingbiao, Li, Yaoxian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2019
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076481/
https://www.ncbi.nlm.nih.gov/pubmed/35541628
http://dx.doi.org/10.1039/c9ra08555f
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author Qi, Shaolong
Zhu, Lubao
Wang, Xinyu
Du, Jianshi
Yang, Qingbiao
Li, Yaoxian
author_facet Qi, Shaolong
Zhu, Lubao
Wang, Xinyu
Du, Jianshi
Yang, Qingbiao
Li, Yaoxian
author_sort Qi, Shaolong
collection PubMed
description Near-infrared (NIR) fluorescent probes are widely employed in biological detection because of their lower damage to biological samples, low background interference, and high signal-to-noise ratio. Herein, a highly water-soluble NIR probe (NIRHA) based on a hemicyanine skeleton and bearing an acrylate moiety was synthesized. The probe showed high selectivity toward cysteine (Cys) over homocysteine (Hcy) and glutathione (GSH). The probe also had low cytotoxicity and was successfully applied in HeLa cells and mouse experiments. Results of bioimaging experiments indicated that the probe was effective for visualizing endogenous Cys in vitro and in vivo.
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spelling pubmed-90764812022-05-09 Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging Qi, Shaolong Zhu, Lubao Wang, Xinyu Du, Jianshi Yang, Qingbiao Li, Yaoxian RSC Adv Chemistry Near-infrared (NIR) fluorescent probes are widely employed in biological detection because of their lower damage to biological samples, low background interference, and high signal-to-noise ratio. Herein, a highly water-soluble NIR probe (NIRHA) based on a hemicyanine skeleton and bearing an acrylate moiety was synthesized. The probe showed high selectivity toward cysteine (Cys) over homocysteine (Hcy) and glutathione (GSH). The probe also had low cytotoxicity and was successfully applied in HeLa cells and mouse experiments. Results of bioimaging experiments indicated that the probe was effective for visualizing endogenous Cys in vitro and in vivo. The Royal Society of Chemistry 2019-12-16 /pmc/articles/PMC9076481/ /pubmed/35541628 http://dx.doi.org/10.1039/c9ra08555f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Qi, Shaolong
Zhu, Lubao
Wang, Xinyu
Du, Jianshi
Yang, Qingbiao
Li, Yaoxian
Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging
title Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging
title_full Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging
title_fullStr Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging
title_full_unstemmed Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging
title_short Near-infrared turn-on fluorescent probe for discriminative detection of Cys and application in in vivo imaging
title_sort near-infrared turn-on fluorescent probe for discriminative detection of cys and application in in vivo imaging
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076481/
https://www.ncbi.nlm.nih.gov/pubmed/35541628
http://dx.doi.org/10.1039/c9ra08555f
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