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A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita

Guanosine triphosphate (GTP) cyclohydrolase I (GCH1) is the limiting enzyme of the tetrahydrobiopterin (BH4) synthesis pathway. The disruption of gch1 gene may cause conditional lethality due to folic acid auxotrophy in microorganisms, although the function of gch1 in basidiomycetes has not been dec...

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Autores principales: Tao, Nan, Cheng, Bopu, Chai, Hongmei, Cui, Xianghua, Ma, Yuanhao, Yan, Jinping, Zhao, Yongchang, Chen, Weimin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076582/
https://www.ncbi.nlm.nih.gov/pubmed/35535251
http://dx.doi.org/10.3389/fmicb.2022.870658
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author Tao, Nan
Cheng, Bopu
Chai, Hongmei
Cui, Xianghua
Ma, Yuanhao
Yan, Jinping
Zhao, Yongchang
Chen, Weimin
author_facet Tao, Nan
Cheng, Bopu
Chai, Hongmei
Cui, Xianghua
Ma, Yuanhao
Yan, Jinping
Zhao, Yongchang
Chen, Weimin
author_sort Tao, Nan
collection PubMed
description Guanosine triphosphate (GTP) cyclohydrolase I (GCH1) is the limiting enzyme of the tetrahydrobiopterin (BH4) synthesis pathway. The disruption of gch1 gene may cause conditional lethality due to folic acid auxotrophy in microorganisms, although the function of gch1 in basidiomycetes has not been deciphered so far. In the present study, gch1 expression in Cyclocybe aegerita (cagch1) was downregulated using the RNAi method, which resulted in growth retardation in both solid and liquid medium, with the hyphal tips exhibiting increased branching compared to that in the wild strain. The development of fruiting bodies in the mutant strains was significantly blocked, and there were short and bottle-shaped stipes. The transcriptional profile revealed that the genes of the MAPK pathway may be involved in the regulation of these effects caused by cagch1 knockdown, which provided an opportunity to study the role of gch1 in the development process of basidiomycetes.
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spelling pubmed-90765822022-05-08 A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita Tao, Nan Cheng, Bopu Chai, Hongmei Cui, Xianghua Ma, Yuanhao Yan, Jinping Zhao, Yongchang Chen, Weimin Front Microbiol Microbiology Guanosine triphosphate (GTP) cyclohydrolase I (GCH1) is the limiting enzyme of the tetrahydrobiopterin (BH4) synthesis pathway. The disruption of gch1 gene may cause conditional lethality due to folic acid auxotrophy in microorganisms, although the function of gch1 in basidiomycetes has not been deciphered so far. In the present study, gch1 expression in Cyclocybe aegerita (cagch1) was downregulated using the RNAi method, which resulted in growth retardation in both solid and liquid medium, with the hyphal tips exhibiting increased branching compared to that in the wild strain. The development of fruiting bodies in the mutant strains was significantly blocked, and there were short and bottle-shaped stipes. The transcriptional profile revealed that the genes of the MAPK pathway may be involved in the regulation of these effects caused by cagch1 knockdown, which provided an opportunity to study the role of gch1 in the development process of basidiomycetes. Frontiers Media S.A. 2022-04-22 /pmc/articles/PMC9076582/ /pubmed/35535251 http://dx.doi.org/10.3389/fmicb.2022.870658 Text en Copyright © 2022 Tao, Cheng, Chai, Cui, Ma, Yan, Zhao and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Tao, Nan
Cheng, Bopu
Chai, Hongmei
Cui, Xianghua
Ma, Yuanhao
Yan, Jinping
Zhao, Yongchang
Chen, Weimin
A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita
title A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita
title_full A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita
title_fullStr A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita
title_full_unstemmed A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita
title_short A Putative Guanosine Triphosphate Cyclohydrolase I Named CaGCH1 Is Involved in Hyphal Branching and Fruiting Development in Cyclocybe aegerita
title_sort putative guanosine triphosphate cyclohydrolase i named cagch1 is involved in hyphal branching and fruiting development in cyclocybe aegerita
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076582/
https://www.ncbi.nlm.nih.gov/pubmed/35535251
http://dx.doi.org/10.3389/fmicb.2022.870658
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