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Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products
A hydrothermal sol–gel method for reproducible formation of silica nanolayer on the wall of silica capillaries was developed for electrochromatography. The formulation was optimized by observation of uniform gel formation on an optical microscope. The variables of the formulation include types of so...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076594/ https://www.ncbi.nlm.nih.gov/pubmed/35523909 http://dx.doi.org/10.1038/s41598-022-11078-y |
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author | Obma, Apinya Hemwech, Pattamaporn Phoolpho, Sittisak Bumrungpuech, Rawiwan Wirasate, Supa Kaowphong, Sulawan Wilairat, Prapin Chantiwas, Rattikan |
author_facet | Obma, Apinya Hemwech, Pattamaporn Phoolpho, Sittisak Bumrungpuech, Rawiwan Wirasate, Supa Kaowphong, Sulawan Wilairat, Prapin Chantiwas, Rattikan |
author_sort | Obma, Apinya |
collection | PubMed |
description | A hydrothermal sol–gel method for reproducible formation of silica nanolayer on the wall of silica capillaries was developed for electrochromatography. The formulation was optimized by observation of uniform gel formation on an optical microscope. The variables of the formulation include types of solvent, water-TEOS ratio, CTAB and urea contents, and mixing method. The procedure produced a coating of silica ca. 100 nm thick layer on the wall of the capillary. Surface morphology of the coating was characterized by SEM, contact angle and chemical composition by FT-IR spectroscopy and X-ray powder diffraction. The coating reduced the electroosmotic mobility producing enhanced separation performance. Eight standard amines (including tyramine and benzhydrylamine, as an internal standard) were separated with peak resolution R(s) ≥ 2 for all adjacent peaks and plate number N ≥ 3.0 × 10(4) m(-1). Calibration was linear from 5 to 200 µg L(-1), with r(2) > 0.9985 and instrumental LOD of 4.9 μg L(-1). Five samples of food products were diluted and analyzed for the amines using the coated capillary and only tyramine was detected. Intra-day and inter-day precisions were less than 1.2%RSD. Percent recoveries of spiked tyramine in samples were 95 ± 3 to 106 ± 7% (n = 3). |
format | Online Article Text |
id | pubmed-9076594 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90765942022-05-08 Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products Obma, Apinya Hemwech, Pattamaporn Phoolpho, Sittisak Bumrungpuech, Rawiwan Wirasate, Supa Kaowphong, Sulawan Wilairat, Prapin Chantiwas, Rattikan Sci Rep Article A hydrothermal sol–gel method for reproducible formation of silica nanolayer on the wall of silica capillaries was developed for electrochromatography. The formulation was optimized by observation of uniform gel formation on an optical microscope. The variables of the formulation include types of solvent, water-TEOS ratio, CTAB and urea contents, and mixing method. The procedure produced a coating of silica ca. 100 nm thick layer on the wall of the capillary. Surface morphology of the coating was characterized by SEM, contact angle and chemical composition by FT-IR spectroscopy and X-ray powder diffraction. The coating reduced the electroosmotic mobility producing enhanced separation performance. Eight standard amines (including tyramine and benzhydrylamine, as an internal standard) were separated with peak resolution R(s) ≥ 2 for all adjacent peaks and plate number N ≥ 3.0 × 10(4) m(-1). Calibration was linear from 5 to 200 µg L(-1), with r(2) > 0.9985 and instrumental LOD of 4.9 μg L(-1). Five samples of food products were diluted and analyzed for the amines using the coated capillary and only tyramine was detected. Intra-day and inter-day precisions were less than 1.2%RSD. Percent recoveries of spiked tyramine in samples were 95 ± 3 to 106 ± 7% (n = 3). Nature Publishing Group UK 2022-05-06 /pmc/articles/PMC9076594/ /pubmed/35523909 http://dx.doi.org/10.1038/s41598-022-11078-y Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Obma, Apinya Hemwech, Pattamaporn Phoolpho, Sittisak Bumrungpuech, Rawiwan Wirasate, Supa Kaowphong, Sulawan Wilairat, Prapin Chantiwas, Rattikan Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products |
title | Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products |
title_full | Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products |
title_fullStr | Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products |
title_full_unstemmed | Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products |
title_short | Silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products |
title_sort | silica nanolayer coated capillary by hydrothermal sol–gel process for amines separation and detection of tyramine in food products |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076594/ https://www.ncbi.nlm.nih.gov/pubmed/35523909 http://dx.doi.org/10.1038/s41598-022-11078-y |
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