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Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions
Newly synthesized H3.1 and H3.3 histones are assembled into nucleosomes by different histone chaperones in replication-coupled and replication-independent pathways, respectively. However, it is not clear how parental H3.3 molecules are transferred following DNA replication, especially when compared...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076889/ https://www.ncbi.nlm.nih.gov/pubmed/35523900 http://dx.doi.org/10.1038/s41467-022-30298-4 |
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author | Xu, Xiaowei Duan, Shoufu Hua, Xu Li, Zhiming He, Richard Zhang, Zhiguo |
author_facet | Xu, Xiaowei Duan, Shoufu Hua, Xu Li, Zhiming He, Richard Zhang, Zhiguo |
author_sort | Xu, Xiaowei |
collection | PubMed |
description | Newly synthesized H3.1 and H3.3 histones are assembled into nucleosomes by different histone chaperones in replication-coupled and replication-independent pathways, respectively. However, it is not clear how parental H3.3 molecules are transferred following DNA replication, especially when compared to H3.1. Here, by monitoring parental H3.1- and H3.3-SNAP signals, we show that parental H3.3, like H3.1, are stably transferred into daughter cells. Moreover, Mcm2-Pola1 and Pole3-Pole4, two pathways involved in parental histone transfer based upon the analysis of modifications on parental histones, participate in the transfer of both H3.1 and H3.3 following DNA replication. Lastly, we found that Mcm2, Pole3 and Pole4 mutants defective in parental histone transfer show defects in chromosome segregation. These results indicate that in contrast to deposition of newly synthesized H3.1 and H3.3, transfer of parental H3.1 and H3.3 is mediated by these shared mechanisms, which contributes to epigenetic memory of gene expression and maintenance of genome stability. |
format | Online Article Text |
id | pubmed-9076889 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90768892022-05-08 Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions Xu, Xiaowei Duan, Shoufu Hua, Xu Li, Zhiming He, Richard Zhang, Zhiguo Nat Commun Article Newly synthesized H3.1 and H3.3 histones are assembled into nucleosomes by different histone chaperones in replication-coupled and replication-independent pathways, respectively. However, it is not clear how parental H3.3 molecules are transferred following DNA replication, especially when compared to H3.1. Here, by monitoring parental H3.1- and H3.3-SNAP signals, we show that parental H3.3, like H3.1, are stably transferred into daughter cells. Moreover, Mcm2-Pola1 and Pole3-Pole4, two pathways involved in parental histone transfer based upon the analysis of modifications on parental histones, participate in the transfer of both H3.1 and H3.3 following DNA replication. Lastly, we found that Mcm2, Pole3 and Pole4 mutants defective in parental histone transfer show defects in chromosome segregation. These results indicate that in contrast to deposition of newly synthesized H3.1 and H3.3, transfer of parental H3.1 and H3.3 is mediated by these shared mechanisms, which contributes to epigenetic memory of gene expression and maintenance of genome stability. Nature Publishing Group UK 2022-05-06 /pmc/articles/PMC9076889/ /pubmed/35523900 http://dx.doi.org/10.1038/s41467-022-30298-4 Text en © The Author(s) 2022, corrected publication 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Xu, Xiaowei Duan, Shoufu Hua, Xu Li, Zhiming He, Richard Zhang, Zhiguo Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions |
title | Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions |
title_full | Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions |
title_fullStr | Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions |
title_full_unstemmed | Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions |
title_short | Stable inheritance of H3.3-containing nucleosomes during mitotic cell divisions |
title_sort | stable inheritance of h3.3-containing nucleosomes during mitotic cell divisions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9076889/ https://www.ncbi.nlm.nih.gov/pubmed/35523900 http://dx.doi.org/10.1038/s41467-022-30298-4 |
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