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Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system

Brain organoids derived from the self-organization of human induced pluripotent stem cells (hiPSCs) represent a new class of in vitro organ system for modeling brain development and diseases. However, engineering brain organoids in a biomimetic environment that is favorable for brain development rem...

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Autores principales: Wang, Yaqing, Wang, Li, Guo, Yaqiong, Zhu, Yujuan, Qin, Jianhua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077091/
https://www.ncbi.nlm.nih.gov/pubmed/35540867
http://dx.doi.org/10.1039/c7ra11714k
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author Wang, Yaqing
Wang, Li
Guo, Yaqiong
Zhu, Yujuan
Qin, Jianhua
author_facet Wang, Yaqing
Wang, Li
Guo, Yaqiong
Zhu, Yujuan
Qin, Jianhua
author_sort Wang, Yaqing
collection PubMed
description Brain organoids derived from the self-organization of human induced pluripotent stem cells (hiPSCs) represent a new class of in vitro organ system for modeling brain development and diseases. However, engineering brain organoids in a biomimetic environment that is favorable for brain development remains challenging. In this work, we present a new strategy to generate hiPSCs-derived 3D brain organoids using an organ-on-a-chip system in a controlled manner. This system provides a biomimetic brain microenvironment by incorporating three-dimensional (3D) Matrigel, fluid flow and multicellular architectures of tissues that allows for extended 3D culture, in situ neural differentiation, and organization of brain organoids on a single device. The generated brain organoids display well-defined neural differentiation, regionalization and cortical organization under perfused culture conditions, which recapitulate the key features of early human brain development. Moreover, the brain organoids exhibit an enhanced expression of cortical layer markers (TBR1 and CTIP2) under perfused cultures as compared to that under static cultures on a Petri dish, indicating the role of mechanical fluid flow in promoting brain organogenesis. The simple and robust brain organoids-on-a-chip system may open new avenues for various stem cell-based organoids engineering and its application in developmental biology and human disease studies.
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spelling pubmed-90770912022-05-09 Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system Wang, Yaqing Wang, Li Guo, Yaqiong Zhu, Yujuan Qin, Jianhua RSC Adv Chemistry Brain organoids derived from the self-organization of human induced pluripotent stem cells (hiPSCs) represent a new class of in vitro organ system for modeling brain development and diseases. However, engineering brain organoids in a biomimetic environment that is favorable for brain development remains challenging. In this work, we present a new strategy to generate hiPSCs-derived 3D brain organoids using an organ-on-a-chip system in a controlled manner. This system provides a biomimetic brain microenvironment by incorporating three-dimensional (3D) Matrigel, fluid flow and multicellular architectures of tissues that allows for extended 3D culture, in situ neural differentiation, and organization of brain organoids on a single device. The generated brain organoids display well-defined neural differentiation, regionalization and cortical organization under perfused culture conditions, which recapitulate the key features of early human brain development. Moreover, the brain organoids exhibit an enhanced expression of cortical layer markers (TBR1 and CTIP2) under perfused cultures as compared to that under static cultures on a Petri dish, indicating the role of mechanical fluid flow in promoting brain organogenesis. The simple and robust brain organoids-on-a-chip system may open new avenues for various stem cell-based organoids engineering and its application in developmental biology and human disease studies. The Royal Society of Chemistry 2018-01-05 /pmc/articles/PMC9077091/ /pubmed/35540867 http://dx.doi.org/10.1039/c7ra11714k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Wang, Yaqing
Wang, Li
Guo, Yaqiong
Zhu, Yujuan
Qin, Jianhua
Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
title Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
title_full Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
title_fullStr Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
title_full_unstemmed Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
title_short Engineering stem cell-derived 3D brain organoids in a perfusable organ-on-a-chip system
title_sort engineering stem cell-derived 3d brain organoids in a perfusable organ-on-a-chip system
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077091/
https://www.ncbi.nlm.nih.gov/pubmed/35540867
http://dx.doi.org/10.1039/c7ra11714k
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