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A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots
Based on the strong interaction between single-stranded DNA and graphene material, we have designed a simple but smart electrochemical platform to detect HBV-DNA by using a graphene quantum dot (GQD) modified glassy carbon electrode coupled with specific sequence DNA molecules as probes. The probe D...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077103/ https://www.ncbi.nlm.nih.gov/pubmed/35542626 http://dx.doi.org/10.1039/c7ra11945c |
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author | Xiang, Qian Huang, Jingyun Huang, Huiyao Mao, Weiwei Ye, Zhizhen |
author_facet | Xiang, Qian Huang, Jingyun Huang, Huiyao Mao, Weiwei Ye, Zhizhen |
author_sort | Xiang, Qian |
collection | PubMed |
description | Based on the strong interaction between single-stranded DNA and graphene material, we have designed a simple but smart electrochemical platform to detect HBV-DNA by using a graphene quantum dot (GQD) modified glassy carbon electrode coupled with specific sequence DNA molecules as probes. The probe DNA is designed to be complementary to the HBV-DNA, when the probe DNA is strongly bound to the surface of the GQD modified electrode the transfer of an electron from the electrode to the electrochemically active species K(3)[Fe(CN)(6)] will become difficult. Nevertheless, if the target HBV-DNA is found in the test solution, the probe DNA will bind with the target HBV-DNA instead of GQDs. As a result, the obtained peak currents of K(3)[Fe(CN)(6)] will have a different degree of increase with the different concentrations of the target HBV-DNA. In particular, the proposed sensor exhibits high sensitivity with a detection limit of 1 nM, and the linear detection range is from 10 nM to 500 nM. Additionally, the sensor could be used in detecting other probe DNA, which may have potential applications in the future. |
format | Online Article Text |
id | pubmed-9077103 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90771032022-05-09 A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots Xiang, Qian Huang, Jingyun Huang, Huiyao Mao, Weiwei Ye, Zhizhen RSC Adv Chemistry Based on the strong interaction between single-stranded DNA and graphene material, we have designed a simple but smart electrochemical platform to detect HBV-DNA by using a graphene quantum dot (GQD) modified glassy carbon electrode coupled with specific sequence DNA molecules as probes. The probe DNA is designed to be complementary to the HBV-DNA, when the probe DNA is strongly bound to the surface of the GQD modified electrode the transfer of an electron from the electrode to the electrochemically active species K(3)[Fe(CN)(6)] will become difficult. Nevertheless, if the target HBV-DNA is found in the test solution, the probe DNA will bind with the target HBV-DNA instead of GQDs. As a result, the obtained peak currents of K(3)[Fe(CN)(6)] will have a different degree of increase with the different concentrations of the target HBV-DNA. In particular, the proposed sensor exhibits high sensitivity with a detection limit of 1 nM, and the linear detection range is from 10 nM to 500 nM. Additionally, the sensor could be used in detecting other probe DNA, which may have potential applications in the future. The Royal Society of Chemistry 2018-01-08 /pmc/articles/PMC9077103/ /pubmed/35542626 http://dx.doi.org/10.1039/c7ra11945c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Xiang, Qian Huang, Jingyun Huang, Huiyao Mao, Weiwei Ye, Zhizhen A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots |
title | A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots |
title_full | A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots |
title_fullStr | A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots |
title_full_unstemmed | A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots |
title_short | A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots |
title_sort | label-free electrochemical platform for the highly sensitive detection of hepatitis b virus dna using graphene quantum dots |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077103/ https://www.ncbi.nlm.nih.gov/pubmed/35542626 http://dx.doi.org/10.1039/c7ra11945c |
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