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A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots

Based on the strong interaction between single-stranded DNA and graphene material, we have designed a simple but smart electrochemical platform to detect HBV-DNA by using a graphene quantum dot (GQD) modified glassy carbon electrode coupled with specific sequence DNA molecules as probes. The probe D...

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Detalles Bibliográficos
Autores principales: Xiang, Qian, Huang, Jingyun, Huang, Huiyao, Mao, Weiwei, Ye, Zhizhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077103/
https://www.ncbi.nlm.nih.gov/pubmed/35542626
http://dx.doi.org/10.1039/c7ra11945c
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author Xiang, Qian
Huang, Jingyun
Huang, Huiyao
Mao, Weiwei
Ye, Zhizhen
author_facet Xiang, Qian
Huang, Jingyun
Huang, Huiyao
Mao, Weiwei
Ye, Zhizhen
author_sort Xiang, Qian
collection PubMed
description Based on the strong interaction between single-stranded DNA and graphene material, we have designed a simple but smart electrochemical platform to detect HBV-DNA by using a graphene quantum dot (GQD) modified glassy carbon electrode coupled with specific sequence DNA molecules as probes. The probe DNA is designed to be complementary to the HBV-DNA, when the probe DNA is strongly bound to the surface of the GQD modified electrode the transfer of an electron from the electrode to the electrochemically active species K(3)[Fe(CN)(6)] will become difficult. Nevertheless, if the target HBV-DNA is found in the test solution, the probe DNA will bind with the target HBV-DNA instead of GQDs. As a result, the obtained peak currents of K(3)[Fe(CN)(6)] will have a different degree of increase with the different concentrations of the target HBV-DNA. In particular, the proposed sensor exhibits high sensitivity with a detection limit of 1 nM, and the linear detection range is from 10 nM to 500 nM. Additionally, the sensor could be used in detecting other probe DNA, which may have potential applications in the future.
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spelling pubmed-90771032022-05-09 A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots Xiang, Qian Huang, Jingyun Huang, Huiyao Mao, Weiwei Ye, Zhizhen RSC Adv Chemistry Based on the strong interaction between single-stranded DNA and graphene material, we have designed a simple but smart electrochemical platform to detect HBV-DNA by using a graphene quantum dot (GQD) modified glassy carbon electrode coupled with specific sequence DNA molecules as probes. The probe DNA is designed to be complementary to the HBV-DNA, when the probe DNA is strongly bound to the surface of the GQD modified electrode the transfer of an electron from the electrode to the electrochemically active species K(3)[Fe(CN)(6)] will become difficult. Nevertheless, if the target HBV-DNA is found in the test solution, the probe DNA will bind with the target HBV-DNA instead of GQDs. As a result, the obtained peak currents of K(3)[Fe(CN)(6)] will have a different degree of increase with the different concentrations of the target HBV-DNA. In particular, the proposed sensor exhibits high sensitivity with a detection limit of 1 nM, and the linear detection range is from 10 nM to 500 nM. Additionally, the sensor could be used in detecting other probe DNA, which may have potential applications in the future. The Royal Society of Chemistry 2018-01-08 /pmc/articles/PMC9077103/ /pubmed/35542626 http://dx.doi.org/10.1039/c7ra11945c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Xiang, Qian
Huang, Jingyun
Huang, Huiyao
Mao, Weiwei
Ye, Zhizhen
A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots
title A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots
title_full A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots
title_fullStr A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots
title_full_unstemmed A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots
title_short A label-free electrochemical platform for the highly sensitive detection of hepatitis B virus DNA using graphene quantum dots
title_sort label-free electrochemical platform for the highly sensitive detection of hepatitis b virus dna using graphene quantum dots
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077103/
https://www.ncbi.nlm.nih.gov/pubmed/35542626
http://dx.doi.org/10.1039/c7ra11945c
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