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Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene
The efficacy of polyether-ether-ketone (PEEK) as a bearing material in knee components, a potential alternative to the currently used highly cross-linked polyethylene (HXLPE), has attracted a lot of attention recently. This study aimed to systematically assess the effect of particulate wear debris o...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077207/ https://www.ncbi.nlm.nih.gov/pubmed/35542597 http://dx.doi.org/10.1039/c7ra10589d |
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author | Du, Zhe Wang, Shujun Wang, You |
author_facet | Du, Zhe Wang, Shujun Wang, You |
author_sort | Du, Zhe |
collection | PubMed |
description | The efficacy of polyether-ether-ketone (PEEK) as a bearing material in knee components, a potential alternative to the currently used highly cross-linked polyethylene (HXLPE), has attracted a lot of attention recently. This study aimed to systematically assess the effect of particulate wear debris on CD4 and CD8 T-cell responses. HXLPE and PEEK particles (96% less than 5 μm) were generated by custom cryo-milling and pulverization in liquid nitrogen, and then incubated with blood collected from 25 donors. The phenotypes of the T-cells were systematically analyzed by immunostaining and flow cytometry. For the in vivo study, 0.1 mL of each particle suspension (about 1.0 × 10(8) wear particles) was injected into murine knee joints; the synovium and spleen were collected one week later for histological examination and immunofluorescence staining. PEEK and HXLPE particles did not induce CD4+ T-cell responses; however, CD8+ T-cells might be involved in mediating particle-induced reactions. The T-cell and inflammatory responses induced by PEEK and HXLPE particles were comparable. Further investigations into the frictional properties of materials should be performed to expand on our results. |
format | Online Article Text |
id | pubmed-9077207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90772072022-05-09 Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene Du, Zhe Wang, Shujun Wang, You RSC Adv Chemistry The efficacy of polyether-ether-ketone (PEEK) as a bearing material in knee components, a potential alternative to the currently used highly cross-linked polyethylene (HXLPE), has attracted a lot of attention recently. This study aimed to systematically assess the effect of particulate wear debris on CD4 and CD8 T-cell responses. HXLPE and PEEK particles (96% less than 5 μm) were generated by custom cryo-milling and pulverization in liquid nitrogen, and then incubated with blood collected from 25 donors. The phenotypes of the T-cells were systematically analyzed by immunostaining and flow cytometry. For the in vivo study, 0.1 mL of each particle suspension (about 1.0 × 10(8) wear particles) was injected into murine knee joints; the synovium and spleen were collected one week later for histological examination and immunofluorescence staining. PEEK and HXLPE particles did not induce CD4+ T-cell responses; however, CD8+ T-cells might be involved in mediating particle-induced reactions. The T-cell and inflammatory responses induced by PEEK and HXLPE particles were comparable. Further investigations into the frictional properties of materials should be performed to expand on our results. The Royal Society of Chemistry 2018-01-09 /pmc/articles/PMC9077207/ /pubmed/35542597 http://dx.doi.org/10.1039/c7ra10589d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Du, Zhe Wang, Shujun Wang, You Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene |
title | Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene |
title_full | Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene |
title_fullStr | Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene |
title_full_unstemmed | Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene |
title_short | Preferential CD8 rather than CD4 T-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene |
title_sort | preferential cd8 rather than cd4 t-cell response to wear particles of polyether-ether-ketone and highly cross-linked polyethylene |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077207/ https://www.ncbi.nlm.nih.gov/pubmed/35542597 http://dx.doi.org/10.1039/c7ra10589d |
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