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Glucose monitoring in living cells with single fluorescent protein-based sensors
Glucose is the main source of energy and carbon in organisms and plays a central role in metabolism and cellular homeostasis. However, the sensitive fluctuation of glucose in living cells is difficult to monitor. Thus, we developed a series of ratiometric, highly responsive, single fluorescent prote...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077360/ https://www.ncbi.nlm.nih.gov/pubmed/35541484 http://dx.doi.org/10.1039/c7ra11347a |
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author | Hu, Hanyang Wei, Yufeng Wang, Daocheng Su, Ni Chen, Xianjun Zhao, Yuzheng Liu, Guixia Yang, Yi |
author_facet | Hu, Hanyang Wei, Yufeng Wang, Daocheng Su, Ni Chen, Xianjun Zhao, Yuzheng Liu, Guixia Yang, Yi |
author_sort | Hu, Hanyang |
collection | PubMed |
description | Glucose is the main source of energy and carbon in organisms and plays a central role in metabolism and cellular homeostasis. However, the sensitive fluctuation of glucose in living cells is difficult to monitor. Thus, we developed a series of ratiometric, highly responsive, single fluorescent protein-based glucose sensors of wide dynamic range by combining a circularly permuted yellow fluorescent protein with a bacterial periplasmic glucose/galactose-binding protein. We used these sensors to monitor glucose transport in living Escherichia coli cells, and found that the cells take up glucose within 10 min to maintain physiological glucose levels, and observed the differences in glucose uptake and glucose metabolism between wild-type and Mlc knockout cells. These sensors can be specific and simple tools for glucose detection in vitro and non-invasive tools for real-time monitoring of glucose metabolism in vivo. |
format | Online Article Text |
id | pubmed-9077360 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90773602022-05-09 Glucose monitoring in living cells with single fluorescent protein-based sensors Hu, Hanyang Wei, Yufeng Wang, Daocheng Su, Ni Chen, Xianjun Zhao, Yuzheng Liu, Guixia Yang, Yi RSC Adv Chemistry Glucose is the main source of energy and carbon in organisms and plays a central role in metabolism and cellular homeostasis. However, the sensitive fluctuation of glucose in living cells is difficult to monitor. Thus, we developed a series of ratiometric, highly responsive, single fluorescent protein-based glucose sensors of wide dynamic range by combining a circularly permuted yellow fluorescent protein with a bacterial periplasmic glucose/galactose-binding protein. We used these sensors to monitor glucose transport in living Escherichia coli cells, and found that the cells take up glucose within 10 min to maintain physiological glucose levels, and observed the differences in glucose uptake and glucose metabolism between wild-type and Mlc knockout cells. These sensors can be specific and simple tools for glucose detection in vitro and non-invasive tools for real-time monitoring of glucose metabolism in vivo. The Royal Society of Chemistry 2018-01-10 /pmc/articles/PMC9077360/ /pubmed/35541484 http://dx.doi.org/10.1039/c7ra11347a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Hu, Hanyang Wei, Yufeng Wang, Daocheng Su, Ni Chen, Xianjun Zhao, Yuzheng Liu, Guixia Yang, Yi Glucose monitoring in living cells with single fluorescent protein-based sensors |
title | Glucose monitoring in living cells with single fluorescent protein-based sensors |
title_full | Glucose monitoring in living cells with single fluorescent protein-based sensors |
title_fullStr | Glucose monitoring in living cells with single fluorescent protein-based sensors |
title_full_unstemmed | Glucose monitoring in living cells with single fluorescent protein-based sensors |
title_short | Glucose monitoring in living cells with single fluorescent protein-based sensors |
title_sort | glucose monitoring in living cells with single fluorescent protein-based sensors |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077360/ https://www.ncbi.nlm.nih.gov/pubmed/35541484 http://dx.doi.org/10.1039/c7ra11347a |
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