Biosynthesis of (R)‐(+)‐perillyl alcohol by Escherichia coli expressing neryl pyrophosphate synthase

(R)‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of (R)‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration...

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Detalles Bibliográficos
Autores principales: Sun, Chao, Zhang, Rubing, Xie, Congxia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9077825/
https://www.ncbi.nlm.nih.gov/pubmed/35573132
http://dx.doi.org/10.1002/elsc.202100135
Descripción
Sumario:(R)‐(+)‐perillyl alcohol is widely used in agricultural and anticarcinogenic fields. Microbial production of (R)‐(+)‐perillyl alcohol was investigated in this study. We optimized biosynthesis of (R)‐(+)‐perillyl alcohol in Escherichia coli by using neryl pyrophosphate synthase and NADPH regeneration. Engineering neryl pyrophosphate (NPP)‐supplied pathway resulted in a 4‐fold improvement of (R)‐(+)‐perillyl alcohol titer. Subsequently, combined engineering of p‐cymene monooxygenase (CymA) expression and module for NADPH regeneration exhibited a 15.4‐fold increase of titer over the initial strain S02. Finally, 453 mg/L (R)‐(+)‐perillyl alcohol was achieved in fed‐batch fermentation, which is the highest (R)‐(+)‐perillyl alcohol titer in E. coli.

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