Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

We have created modified protein variants by introducing a non-canonical amino acid p-azido-l-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b(562) proteins was verified through a combination of atomic force and...

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Detalles Bibliográficos
Autores principales: Zaki, Athraa J., Hartley, Andrew M., Reddington, Samuel C., Thomas, Suzanne K., Watson, Peter, Hayes, Anthony, Moskalenko, Andy V., Craciun, Monica F., Macdonald, J. Emyr, Jones, D. Dafydd, Elliott, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078156/
https://www.ncbi.nlm.nih.gov/pubmed/35539607
http://dx.doi.org/10.1039/c7ra11166e
Descripción
Sumario:We have created modified protein variants by introducing a non-canonical amino acid p-azido-l-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b(562) proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.

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