Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle

We have created modified protein variants by introducing a non-canonical amino acid p-azido-l-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b(562) proteins was verified through a combination of atomic force and...

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Autores principales: Zaki, Athraa J., Hartley, Andrew M., Reddington, Samuel C., Thomas, Suzanne K., Watson, Peter, Hayes, Anthony, Moskalenko, Andy V., Craciun, Monica F., Macdonald, J. Emyr, Jones, D. Dafydd, Elliott, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078156/
https://www.ncbi.nlm.nih.gov/pubmed/35539607
http://dx.doi.org/10.1039/c7ra11166e
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author Zaki, Athraa J.
Hartley, Andrew M.
Reddington, Samuel C.
Thomas, Suzanne K.
Watson, Peter
Hayes, Anthony
Moskalenko, Andy V.
Craciun, Monica F.
Macdonald, J. Emyr
Jones, D. Dafydd
Elliott, Martin
author_facet Zaki, Athraa J.
Hartley, Andrew M.
Reddington, Samuel C.
Thomas, Suzanne K.
Watson, Peter
Hayes, Anthony
Moskalenko, Andy V.
Craciun, Monica F.
Macdonald, J. Emyr
Jones, D. Dafydd
Elliott, Martin
author_sort Zaki, Athraa J.
collection PubMed
description We have created modified protein variants by introducing a non-canonical amino acid p-azido-l-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b(562) proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability.
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spelling pubmed-90781562022-05-09 Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle Zaki, Athraa J. Hartley, Andrew M. Reddington, Samuel C. Thomas, Suzanne K. Watson, Peter Hayes, Anthony Moskalenko, Andy V. Craciun, Monica F. Macdonald, J. Emyr Jones, D. Dafydd Elliott, Martin RSC Adv Chemistry We have created modified protein variants by introducing a non-canonical amino acid p-azido-l-phenylalanine (azF) into defined positions for photochemically-induced covalent attachment to graphene. Attachment of GFP, TEM and cyt b(562) proteins was verified through a combination of atomic force and scanning tunnelling microscopy, resistance measurements, Raman data and fluorescence measurements. This method can in principle be extended to any protein which can be engineered in this way without adversely affecting its structural stability. The Royal Society of Chemistry 2018-02-05 /pmc/articles/PMC9078156/ /pubmed/35539607 http://dx.doi.org/10.1039/c7ra11166e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Zaki, Athraa J.
Hartley, Andrew M.
Reddington, Samuel C.
Thomas, Suzanne K.
Watson, Peter
Hayes, Anthony
Moskalenko, Andy V.
Craciun, Monica F.
Macdonald, J. Emyr
Jones, D. Dafydd
Elliott, Martin
Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle
title Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle
title_full Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle
title_fullStr Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle
title_full_unstemmed Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle
title_short Defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle
title_sort defined covalent assembly of protein molecules on graphene using a genetically encoded photochemical reaction handle
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078156/
https://www.ncbi.nlm.nih.gov/pubmed/35539607
http://dx.doi.org/10.1039/c7ra11166e
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