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Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry

Ursolic acid (UA), a pentacyclic terpenoid carboxylic acid widely existing in various medicinal plants, has been reported to have multifarious biological activities such as anti-inflammatory, anticancer and antioxidant activities. In this paper, we analyzed the metabolic profile of UA in mice (inclu...

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Autores principales: Hu, Xueyan, Shen, Yunbing, Yang, Shengnan, Lei, Wei, Luo, Cheng, Hou, Yuanyuan, Bai, Gang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078307/
https://www.ncbi.nlm.nih.gov/pubmed/35540410
http://dx.doi.org/10.1039/c7ra11856b
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author Hu, Xueyan
Shen, Yunbing
Yang, Shengnan
Lei, Wei
Luo, Cheng
Hou, Yuanyuan
Bai, Gang
author_facet Hu, Xueyan
Shen, Yunbing
Yang, Shengnan
Lei, Wei
Luo, Cheng
Hou, Yuanyuan
Bai, Gang
author_sort Hu, Xueyan
collection PubMed
description Ursolic acid (UA), a pentacyclic terpenoid carboxylic acid widely existing in various medicinal plants, has been reported to have multifarious biological activities such as anti-inflammatory, anticancer and antioxidant activities. In this paper, we analyzed the metabolic profile of UA in mice (including plasma and urine) by using ultra-high performance liquid chromatography (UPLC) coupled with a quadrupole time-of-flight (Q/TOF) method. Principal component analysis (PCA) was applied to differentiate the control and experimental groups. Potential biomarkers were filtered by using loading plots followed by further analysis with UPLC-Q/TOF-MS data. The results showed that 3 metabolites in plasma were identified as markers, one of which was UA and the others were UA epoxides, which belonged to phase I metabolites. Additionally, 5 phase II metabolites were tentatively identified in urine through an accurate mass and characteristic fragment ions. These data suggested that the biotransformation of UA undergoes the major metabolic reactions of the phase I metabolic route of olefin oxidation and phase II metabolic routes of glycine conjugation, glutathione conjugation and glucuronidation. This is the first report of analysis and characterization of the metabolites after the oral administration of UA in mice. The proposed metabolic pathways of UA in mice is also raised for the first time. It might provide further understanding of the potential biological mechanism of UA.
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spelling pubmed-90783072022-05-09 Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry Hu, Xueyan Shen, Yunbing Yang, Shengnan Lei, Wei Luo, Cheng Hou, Yuanyuan Bai, Gang RSC Adv Chemistry Ursolic acid (UA), a pentacyclic terpenoid carboxylic acid widely existing in various medicinal plants, has been reported to have multifarious biological activities such as anti-inflammatory, anticancer and antioxidant activities. In this paper, we analyzed the metabolic profile of UA in mice (including plasma and urine) by using ultra-high performance liquid chromatography (UPLC) coupled with a quadrupole time-of-flight (Q/TOF) method. Principal component analysis (PCA) was applied to differentiate the control and experimental groups. Potential biomarkers were filtered by using loading plots followed by further analysis with UPLC-Q/TOF-MS data. The results showed that 3 metabolites in plasma were identified as markers, one of which was UA and the others were UA epoxides, which belonged to phase I metabolites. Additionally, 5 phase II metabolites were tentatively identified in urine through an accurate mass and characteristic fragment ions. These data suggested that the biotransformation of UA undergoes the major metabolic reactions of the phase I metabolic route of olefin oxidation and phase II metabolic routes of glycine conjugation, glutathione conjugation and glucuronidation. This is the first report of analysis and characterization of the metabolites after the oral administration of UA in mice. The proposed metabolic pathways of UA in mice is also raised for the first time. It might provide further understanding of the potential biological mechanism of UA. The Royal Society of Chemistry 2018-02-08 /pmc/articles/PMC9078307/ /pubmed/35540410 http://dx.doi.org/10.1039/c7ra11856b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Hu, Xueyan
Shen, Yunbing
Yang, Shengnan
Lei, Wei
Luo, Cheng
Hou, Yuanyuan
Bai, Gang
Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
title Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
title_full Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
title_fullStr Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
title_full_unstemmed Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
title_short Metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
title_sort metabolite identification of ursolic acid in mouse plasma and urine after oral administration by ultra-high performance liquid chromatography/quadrupole time-of-flight mass spectrometry
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078307/
https://www.ncbi.nlm.nih.gov/pubmed/35540410
http://dx.doi.org/10.1039/c7ra11856b
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