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Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles

Magnetic molecular imprinted nanoparticles (MMIPs), combining the progressiveness of magnetic nanoparticles and surface molecular imprinting technology, have attracted increasing attention because of the high efficiency and specificity in isolation and enrichment of the target protein. This study fo...

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Detalles Bibliográficos
Autores principales: Su, Yujie, Qiu, Bixia, Chang, Cuihua, Li, Xin, Zhang, Mengqi, Zhou, Bei, Yang, Yanjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078349/
https://www.ncbi.nlm.nih.gov/pubmed/35539629
http://dx.doi.org/10.1039/c7ra12457k
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author Su, Yujie
Qiu, Bixia
Chang, Cuihua
Li, Xin
Zhang, Mengqi
Zhou, Bei
Yang, Yanjun
author_facet Su, Yujie
Qiu, Bixia
Chang, Cuihua
Li, Xin
Zhang, Mengqi
Zhou, Bei
Yang, Yanjun
author_sort Su, Yujie
collection PubMed
description Magnetic molecular imprinted nanoparticles (MMIPs), combining the progressiveness of magnetic nanoparticles and surface molecular imprinting technology, have attracted increasing attention because of the high efficiency and specificity in isolation and enrichment of the target protein. This study focused on the preparation of bovine hemoglobin MMIPs with bovine hemoglobin (BHb) as the template protein and the molecular imprinted polymer covering the functional magnetic nanoparticles modified with silane and acrylic groups. The physicochemical characteristics as well as the dynamics and isothermal adsorption properties of the generated nanoparticles were investigated to determine their efficiency and specificity in the adsorption of target protein. The maximum adsorption of the target protein was 169.29 mg g(−1) at a specific pH, which was much larger than those obtained in some other research reports. MMIPs showed favorable selectivity towards BHb in a mixture of three different proteins. The results indicated the significant effects and broad prospects of MMIPs in the isolation and enrichment of specific proteins in the field of food, medicine and biological research.
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spelling pubmed-90783492022-05-09 Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles Su, Yujie Qiu, Bixia Chang, Cuihua Li, Xin Zhang, Mengqi Zhou, Bei Yang, Yanjun RSC Adv Chemistry Magnetic molecular imprinted nanoparticles (MMIPs), combining the progressiveness of magnetic nanoparticles and surface molecular imprinting technology, have attracted increasing attention because of the high efficiency and specificity in isolation and enrichment of the target protein. This study focused on the preparation of bovine hemoglobin MMIPs with bovine hemoglobin (BHb) as the template protein and the molecular imprinted polymer covering the functional magnetic nanoparticles modified with silane and acrylic groups. The physicochemical characteristics as well as the dynamics and isothermal adsorption properties of the generated nanoparticles were investigated to determine their efficiency and specificity in the adsorption of target protein. The maximum adsorption of the target protein was 169.29 mg g(−1) at a specific pH, which was much larger than those obtained in some other research reports. MMIPs showed favorable selectivity towards BHb in a mixture of three different proteins. The results indicated the significant effects and broad prospects of MMIPs in the isolation and enrichment of specific proteins in the field of food, medicine and biological research. The Royal Society of Chemistry 2018-02-07 /pmc/articles/PMC9078349/ /pubmed/35539629 http://dx.doi.org/10.1039/c7ra12457k Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Su, Yujie
Qiu, Bixia
Chang, Cuihua
Li, Xin
Zhang, Mengqi
Zhou, Bei
Yang, Yanjun
Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles
title Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles
title_full Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles
title_fullStr Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles
title_full_unstemmed Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles
title_short Separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles
title_sort separation of bovine hemoglobin using novel magnetic molecular imprinted nanoparticles
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078349/
https://www.ncbi.nlm.nih.gov/pubmed/35539629
http://dx.doi.org/10.1039/c7ra12457k
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