Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes

We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in hig...

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Detalles Bibliográficos
Autores principales: Lee, Ha Jung, Go, Gui Han, Ro, Jong Jin, Kim, Byeang Hyean
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078427/
https://www.ncbi.nlm.nih.gov/pubmed/35539109
http://dx.doi.org/10.1039/c7ra13349a
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author Lee, Ha Jung
Go, Gui Han
Ro, Jong Jin
Kim, Byeang Hyean
author_facet Lee, Ha Jung
Go, Gui Han
Ro, Jong Jin
Kim, Byeang Hyean
author_sort Lee, Ha Jung
collection PubMed
description We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence.
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spelling pubmed-90784272022-05-09 Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes Lee, Ha Jung Go, Gui Han Ro, Jong Jin Kim, Byeang Hyean RSC Adv Chemistry We have developed hybridization-sensitive fluorescent oligonucleotide probes that, in the presence of quencher strands, undergo efficient fluorescence quenching through the formation of partial DNA/DNA duplexes. In the presence of target RNA, rapid displacement of the quencher strands results in highly enhanced fluorescence. The Royal Society of Chemistry 2018-02-16 /pmc/articles/PMC9078427/ /pubmed/35539109 http://dx.doi.org/10.1039/c7ra13349a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Lee, Ha Jung
Go, Gui Han
Ro, Jong Jin
Kim, Byeang Hyean
Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes
title Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes
title_full Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes
title_fullStr Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes
title_full_unstemmed Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes
title_short Detection of cofilin mRNA by hybridization-sensitive double-stranded fluorescent probes
title_sort detection of cofilin mrna by hybridization-sensitive double-stranded fluorescent probes
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078427/
https://www.ncbi.nlm.nih.gov/pubmed/35539109
http://dx.doi.org/10.1039/c7ra13349a
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