Cargando…
Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats
Acute lung injury (ALI) is a severe respiratory disease. To date, no medical interventions have been proven effective in improving the outcome. Reduning injection (RDN) showed a potential effect in the therapy of ALI. However, seldom does research concern the holistic pharmacological mechanisms of R...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078858/ https://www.ncbi.nlm.nih.gov/pubmed/35540831 http://dx.doi.org/10.1039/c7ra13123b |
_version_ | 1784702430691196928 |
---|---|
author | Xiong, Zhili Wang, Yanmin Lang, Lang Ma, Shuping Zhao, Longshan Xiao, Wei Wang, Yanjuan |
author_facet | Xiong, Zhili Wang, Yanmin Lang, Lang Ma, Shuping Zhao, Longshan Xiao, Wei Wang, Yanjuan |
author_sort | Xiong, Zhili |
collection | PubMed |
description | Acute lung injury (ALI) is a severe respiratory disease. To date, no medical interventions have been proven effective in improving the outcome. Reduning injection (RDN) showed a potential effect in the therapy of ALI. However, seldom does research concern the holistic pharmacological mechanisms of RDN on ALI. A metabolomic strategy, based on two consecutive extractions of the lung tissue, has been developed to investigate therapeutic mechanisms of RDN on ALI model rat. The extraction procedure was an aqueous extraction with methanol–water followed by organic extraction with dichloromethane–methanol. According to the lipophilicity of extracts, aqueous extracts were analyzed on the T3 column and organic extracts on the C18 column. Partial least-squares discriminant analysis was utilized to identify differences in metabolic profiles of rats. A total of 14 potential biomarkers in lung tissue were identified, which mainly related to phospholipid metabolism, sphingolipid metabolism, nucleotide metabolism and energy metabolism. The combined analytical method provides complementary metabolomics information for exploring the action mechanism of RDN against ALI. And the obtained results indicate metabolomics is a promising tool for understanding the holism and synergism of traditional Chinese medicine. |
format | Online Article Text |
id | pubmed-9078858 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90788582022-05-09 Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats Xiong, Zhili Wang, Yanmin Lang, Lang Ma, Shuping Zhao, Longshan Xiao, Wei Wang, Yanjuan RSC Adv Chemistry Acute lung injury (ALI) is a severe respiratory disease. To date, no medical interventions have been proven effective in improving the outcome. Reduning injection (RDN) showed a potential effect in the therapy of ALI. However, seldom does research concern the holistic pharmacological mechanisms of RDN on ALI. A metabolomic strategy, based on two consecutive extractions of the lung tissue, has been developed to investigate therapeutic mechanisms of RDN on ALI model rat. The extraction procedure was an aqueous extraction with methanol–water followed by organic extraction with dichloromethane–methanol. According to the lipophilicity of extracts, aqueous extracts were analyzed on the T3 column and organic extracts on the C18 column. Partial least-squares discriminant analysis was utilized to identify differences in metabolic profiles of rats. A total of 14 potential biomarkers in lung tissue were identified, which mainly related to phospholipid metabolism, sphingolipid metabolism, nucleotide metabolism and energy metabolism. The combined analytical method provides complementary metabolomics information for exploring the action mechanism of RDN against ALI. And the obtained results indicate metabolomics is a promising tool for understanding the holism and synergism of traditional Chinese medicine. The Royal Society of Chemistry 2018-03-13 /pmc/articles/PMC9078858/ /pubmed/35540831 http://dx.doi.org/10.1039/c7ra13123b Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Xiong, Zhili Wang, Yanmin Lang, Lang Ma, Shuping Zhao, Longshan Xiao, Wei Wang, Yanjuan Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats |
title | Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats |
title_full | Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats |
title_fullStr | Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats |
title_full_unstemmed | Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats |
title_short | Tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on LPS-induced acute lung injury rats |
title_sort | tissue metabolomic profiling to reveal the therapeutic mechanism of reduning injection on lps-induced acute lung injury rats |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9078858/ https://www.ncbi.nlm.nih.gov/pubmed/35540831 http://dx.doi.org/10.1039/c7ra13123b |
work_keys_str_mv | AT xiongzhili tissuemetabolomicprofilingtorevealthetherapeuticmechanismofreduninginjectiononlpsinducedacutelunginjuryrats AT wangyanmin tissuemetabolomicprofilingtorevealthetherapeuticmechanismofreduninginjectiononlpsinducedacutelunginjuryrats AT langlang tissuemetabolomicprofilingtorevealthetherapeuticmechanismofreduninginjectiononlpsinducedacutelunginjuryrats AT mashuping tissuemetabolomicprofilingtorevealthetherapeuticmechanismofreduninginjectiononlpsinducedacutelunginjuryrats AT zhaolongshan tissuemetabolomicprofilingtorevealthetherapeuticmechanismofreduninginjectiononlpsinducedacutelunginjuryrats AT xiaowei tissuemetabolomicprofilingtorevealthetherapeuticmechanismofreduninginjectiononlpsinducedacutelunginjuryrats AT wangyanjuan tissuemetabolomicprofilingtorevealthetherapeuticmechanismofreduninginjectiononlpsinducedacutelunginjuryrats |