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Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE
Melibiose-derived AGE (MAGE) is an advanced glycation end-product formed in vitro in anhydrous conditions on proteins and protein-free amino acids during glycation with melibiose. Our previous studies revealed the presence of MAGE antigen in the human body and tissues of several other species, inclu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079080/ https://www.ncbi.nlm.nih.gov/pubmed/35525899 http://dx.doi.org/10.1038/s41598-022-11638-2 |
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author | Gostomska-Pampuch, Kinga Gamian, Andrzej Rawicz-Pruszyński, Karol Gęca, Katarzyna Tkaczuk-Włach, Joanna Jonik, Ilona Ożga, Kinga Staniszewska, Magdalena |
author_facet | Gostomska-Pampuch, Kinga Gamian, Andrzej Rawicz-Pruszyński, Karol Gęca, Katarzyna Tkaczuk-Włach, Joanna Jonik, Ilona Ożga, Kinga Staniszewska, Magdalena |
author_sort | Gostomska-Pampuch, Kinga |
collection | PubMed |
description | Melibiose-derived AGE (MAGE) is an advanced glycation end-product formed in vitro in anhydrous conditions on proteins and protein-free amino acids during glycation with melibiose. Our previous studies revealed the presence of MAGE antigen in the human body and tissues of several other species, including muscles, fat, extracellular matrix, and blood. MAGE is also antigenic and induces generation of anti-MAGE antibody. The aim of this paper was to identify the proteins modified by MAGE present in human body fluids, such as serum, plasma, and peritoneal fluids. The protein-bound MAGE formed in vivo has been isolated from human blood using affinity chromatography on the resin with an immobilized anti-MAGE monoclonal antibody. Using mass spectrometry and immunochemistry it has been established that MAGE epitope is present on several human blood proteins including serum albumin, IgG, and IgA. In serum of diabetic patients, mainly the albumin and IgG were modified by MAGE, while in healthy subjects IgG and IgA carried this modification, suggesting the novel AGE can impact protein structure, contribute to auto-immunogenicity, and affect function of immunoglobulins. Some proteins in peritoneal fluid from cancer patients modified with MAGE were also observed and it indicates a potential role of MAGE in cancer. |
format | Online Article Text |
id | pubmed-9079080 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-90790802022-05-09 Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE Gostomska-Pampuch, Kinga Gamian, Andrzej Rawicz-Pruszyński, Karol Gęca, Katarzyna Tkaczuk-Włach, Joanna Jonik, Ilona Ożga, Kinga Staniszewska, Magdalena Sci Rep Article Melibiose-derived AGE (MAGE) is an advanced glycation end-product formed in vitro in anhydrous conditions on proteins and protein-free amino acids during glycation with melibiose. Our previous studies revealed the presence of MAGE antigen in the human body and tissues of several other species, including muscles, fat, extracellular matrix, and blood. MAGE is also antigenic and induces generation of anti-MAGE antibody. The aim of this paper was to identify the proteins modified by MAGE present in human body fluids, such as serum, plasma, and peritoneal fluids. The protein-bound MAGE formed in vivo has been isolated from human blood using affinity chromatography on the resin with an immobilized anti-MAGE monoclonal antibody. Using mass spectrometry and immunochemistry it has been established that MAGE epitope is present on several human blood proteins including serum albumin, IgG, and IgA. In serum of diabetic patients, mainly the albumin and IgG were modified by MAGE, while in healthy subjects IgG and IgA carried this modification, suggesting the novel AGE can impact protein structure, contribute to auto-immunogenicity, and affect function of immunoglobulins. Some proteins in peritoneal fluid from cancer patients modified with MAGE were also observed and it indicates a potential role of MAGE in cancer. Nature Publishing Group UK 2022-05-07 /pmc/articles/PMC9079080/ /pubmed/35525899 http://dx.doi.org/10.1038/s41598-022-11638-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Gostomska-Pampuch, Kinga Gamian, Andrzej Rawicz-Pruszyński, Karol Gęca, Katarzyna Tkaczuk-Włach, Joanna Jonik, Ilona Ożga, Kinga Staniszewska, Magdalena Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE |
title | Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE |
title_full | Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE |
title_fullStr | Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE |
title_full_unstemmed | Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE |
title_short | Proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: MAGE |
title_sort | proteins in human body fluids contain in vivo antigen analog of the melibiose-derived glycation product: mage |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079080/ https://www.ncbi.nlm.nih.gov/pubmed/35525899 http://dx.doi.org/10.1038/s41598-022-11638-2 |
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