A permeability assay for mouse intestinal organoids

Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be i...

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Detalles Bibliográficos
Autores principales: den Daas, Stijn Aaron, Soffientini, Ugo, Chokshi, Shilpa, Mehta, Gautam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079111/
https://www.ncbi.nlm.nih.gov/pubmed/35542176
http://dx.doi.org/10.1016/j.xpro.2022.101365
Descripción
Sumario:Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.

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