A permeability assay for mouse intestinal organoids

Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be i...

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Detalles Bibliográficos
Autores principales: den Daas, Stijn Aaron, Soffientini, Ugo, Chokshi, Shilpa, Mehta, Gautam
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079111/
https://www.ncbi.nlm.nih.gov/pubmed/35542176
http://dx.doi.org/10.1016/j.xpro.2022.101365
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author den Daas, Stijn Aaron
Soffientini, Ugo
Chokshi, Shilpa
Mehta, Gautam
author_facet den Daas, Stijn Aaron
Soffientini, Ugo
Chokshi, Shilpa
Mehta, Gautam
author_sort den Daas, Stijn Aaron
collection PubMed
description Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability.
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spelling pubmed-90791112022-05-09 A permeability assay for mouse intestinal organoids den Daas, Stijn Aaron Soffientini, Ugo Chokshi, Shilpa Mehta, Gautam STAR Protoc Protocol Here, we describe an assay for intestinal permeability in mouse intestinal organoids, although this may also be adapted for other species. Propidium iodide (PI) does not penetrate intact biological membranes and thus cannot enter the lumen of intact organoids. Passage of PI within the lumen can be induced by tight junction disruption or epithelial cell death. This technique measures PI-stained extruded dead cells within the organoid lumen to analyze the effect of insults, toxins, or treatments on intestinal organoid permeability. Elsevier 2022-05-01 /pmc/articles/PMC9079111/ /pubmed/35542176 http://dx.doi.org/10.1016/j.xpro.2022.101365 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
den Daas, Stijn Aaron
Soffientini, Ugo
Chokshi, Shilpa
Mehta, Gautam
A permeability assay for mouse intestinal organoids
title A permeability assay for mouse intestinal organoids
title_full A permeability assay for mouse intestinal organoids
title_fullStr A permeability assay for mouse intestinal organoids
title_full_unstemmed A permeability assay for mouse intestinal organoids
title_short A permeability assay for mouse intestinal organoids
title_sort permeability assay for mouse intestinal organoids
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079111/
https://www.ncbi.nlm.nih.gov/pubmed/35542176
http://dx.doi.org/10.1016/j.xpro.2022.101365
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