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Automated 96-well format high throughput colony formation assay for siRNA library screen

The colony formation assay is the gold-standard technique to assess cell viability after treatment with cytotoxic reagents, ionizing radiation, and cytotoxic combinatorial treatments. This protocol describes a high-throughput automated and high-content imaging approach to screen siRNA molecular libr...

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Detalles Bibliográficos
Autores principales:	Hatch, Stephanie B., Prevo, Remko, Chan, Tiffany, Millar, Val, Cornelissen, Bart, Higgins, Geoff, Ebner, Daniel
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079112/ https://www.ncbi.nlm.nih.gov/pubmed/35542177 http://dx.doi.org/10.1016/j.xpro.2022.101355

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