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Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment
The 3D printing technique is suitable for patient-specific implant preparation for bone repair after bone tumor resection. However, improving the survival rate due to tumor recurrence remains a challenge for implants. The macrophage polarization induction to M2-type tumor-associated macrophages (TAM...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079115/ https://www.ncbi.nlm.nih.gov/pubmed/35574049 http://dx.doi.org/10.1016/j.bioactmat.2022.04.028 |
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author | Li, Cuidi Li, Changwei Ma, Zhenjiang Chen, Hongfang Ruan, Huitong Deng, Lianfu Wang, Jinwu Cui, Wenguo |
author_facet | Li, Cuidi Li, Changwei Ma, Zhenjiang Chen, Hongfang Ruan, Huitong Deng, Lianfu Wang, Jinwu Cui, Wenguo |
author_sort | Li, Cuidi |
collection | PubMed |
description | The 3D printing technique is suitable for patient-specific implant preparation for bone repair after bone tumor resection. However, improving the survival rate due to tumor recurrence remains a challenge for implants. The macrophage polarization induction to M2-type tumor-associated macrophages (TAMs) by the tumor microenvironment is a key factor of immunosuppression and tumor recurrence. In this study, a regenerative scaffold regulating the macrophage immune microenvironment and promoting bone regeneration in a dual-stage process for the postoperative treatment of bone tumors was constructed by binding a colony-stimulating factor 1 receptor (CSF-1R) inhibitor GW2580 onto in situ cosslinked hydroxybutylchitosan (HBC)/oxidized chondroitin sulfate (OCS) hydrogel layer covering a 3D printed calcium phosphate scaffold based on electrostatic interaction. The hydrogel layer on scaffold surface not only supplied abundant sulfonic acid groups for stable loading of the inhibitor, but also acted as the cover mask protecting the bone repair part from exposure to unhealthy growth factors in the microenvironment at the early treatment stage. With local prolonged release of inhibitor being realized via the functional material design, CSF-1R, the main pathway that induces polarization of TAMs, can be efficiently blocked, thus regulating the immunosuppressive microenvironment and inhibiting tumor development at a low therapeutic dose. At the later stage of treatment, calcium phosphate component of the scaffold can facilitate the repair of bone defects caused by tumor excision. In conclusion, the difunctional 3D printed bone repair scaffold regulating immune microenvironment in stages proposed a novel approach for bone tumor postoperative treatment. |
format | Online Article Text |
id | pubmed-9079115 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-90791152022-05-13 Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment Li, Cuidi Li, Changwei Ma, Zhenjiang Chen, Hongfang Ruan, Huitong Deng, Lianfu Wang, Jinwu Cui, Wenguo Bioact Mater Article The 3D printing technique is suitable for patient-specific implant preparation for bone repair after bone tumor resection. However, improving the survival rate due to tumor recurrence remains a challenge for implants. The macrophage polarization induction to M2-type tumor-associated macrophages (TAMs) by the tumor microenvironment is a key factor of immunosuppression and tumor recurrence. In this study, a regenerative scaffold regulating the macrophage immune microenvironment and promoting bone regeneration in a dual-stage process for the postoperative treatment of bone tumors was constructed by binding a colony-stimulating factor 1 receptor (CSF-1R) inhibitor GW2580 onto in situ cosslinked hydroxybutylchitosan (HBC)/oxidized chondroitin sulfate (OCS) hydrogel layer covering a 3D printed calcium phosphate scaffold based on electrostatic interaction. The hydrogel layer on scaffold surface not only supplied abundant sulfonic acid groups for stable loading of the inhibitor, but also acted as the cover mask protecting the bone repair part from exposure to unhealthy growth factors in the microenvironment at the early treatment stage. With local prolonged release of inhibitor being realized via the functional material design, CSF-1R, the main pathway that induces polarization of TAMs, can be efficiently blocked, thus regulating the immunosuppressive microenvironment and inhibiting tumor development at a low therapeutic dose. At the later stage of treatment, calcium phosphate component of the scaffold can facilitate the repair of bone defects caused by tumor excision. In conclusion, the difunctional 3D printed bone repair scaffold regulating immune microenvironment in stages proposed a novel approach for bone tumor postoperative treatment. KeAi Publishing 2022-05-02 /pmc/articles/PMC9079115/ /pubmed/35574049 http://dx.doi.org/10.1016/j.bioactmat.2022.04.028 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Li, Cuidi Li, Changwei Ma, Zhenjiang Chen, Hongfang Ruan, Huitong Deng, Lianfu Wang, Jinwu Cui, Wenguo Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment |
title | Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment |
title_full | Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment |
title_fullStr | Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment |
title_full_unstemmed | Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment |
title_short | Regulated macrophage immune microenvironment in 3D printed scaffolds for bone tumor postoperative treatment |
title_sort | regulated macrophage immune microenvironment in 3d printed scaffolds for bone tumor postoperative treatment |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079115/ https://www.ncbi.nlm.nih.gov/pubmed/35574049 http://dx.doi.org/10.1016/j.bioactmat.2022.04.028 |
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