Cargando…
Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo
Liquiritin (LQ), the main bioactive constituent of licorice, is a common flavoring and sweetening agent in food products and has a wide range of pharmacological properties, including antidepressant-like, neuroprotective, anti-cancer and anti-inflammatory properties. This study investigated the metab...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079117/ https://www.ncbi.nlm.nih.gov/pubmed/35542822 http://dx.doi.org/10.1039/c7ra13760e |
_version_ | 1784702492037087232 |
---|---|
author | Zhang, Xia Liang, Caijuan Yin, Jintuo Sun, Yupeng Zhang, Lantong |
author_facet | Zhang, Xia Liang, Caijuan Yin, Jintuo Sun, Yupeng Zhang, Lantong |
author_sort | Zhang, Xia |
collection | PubMed |
description | Liquiritin (LQ), the main bioactive constituent of licorice, is a common flavoring and sweetening agent in food products and has a wide range of pharmacological properties, including antidepressant-like, neuroprotective, anti-cancer and anti-inflammatory properties. This study investigated the metabolic pathways of LQ in vitro (rat liver microsomes) and in vivo (rat model) using ultra high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). Moreover, supplementary tools such as key product ions (KPIs) were employed to search for and identify compounds. As a result, 56 in vivo metabolites and 15 in vitro metabolites were structurally characterized. Oxidation, reduction, hydrolysis, methylation, acetylation, and sulfate and glucuronide conjugation were determined to be the major metabolic pathways of LQ, and there were differences in LQ metabolism in vitro and in vivo. In addition, the in vitro and in vivo metabolic pathways were compared in this study. |
format | Online Article Text |
id | pubmed-9079117 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90791172022-05-09 Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo Zhang, Xia Liang, Caijuan Yin, Jintuo Sun, Yupeng Zhang, Lantong RSC Adv Chemistry Liquiritin (LQ), the main bioactive constituent of licorice, is a common flavoring and sweetening agent in food products and has a wide range of pharmacological properties, including antidepressant-like, neuroprotective, anti-cancer and anti-inflammatory properties. This study investigated the metabolic pathways of LQ in vitro (rat liver microsomes) and in vivo (rat model) using ultra high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS/MS). Moreover, supplementary tools such as key product ions (KPIs) were employed to search for and identify compounds. As a result, 56 in vivo metabolites and 15 in vitro metabolites were structurally characterized. Oxidation, reduction, hydrolysis, methylation, acetylation, and sulfate and glucuronide conjugation were determined to be the major metabolic pathways of LQ, and there were differences in LQ metabolism in vitro and in vivo. In addition, the in vitro and in vivo metabolic pathways were compared in this study. The Royal Society of Chemistry 2018-03-27 /pmc/articles/PMC9079117/ /pubmed/35542822 http://dx.doi.org/10.1039/c7ra13760e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Zhang, Xia Liang, Caijuan Yin, Jintuo Sun, Yupeng Zhang, Lantong Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo |
title | Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo |
title_full | Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo |
title_fullStr | Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo |
title_full_unstemmed | Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo |
title_short | Identification of metabolites of liquiritin in rats by UHPLC-Q-TOF-MS/MS: metabolic profiling and pathway comparison in vitro and in vivo |
title_sort | identification of metabolites of liquiritin in rats by uhplc-q-tof-ms/ms: metabolic profiling and pathway comparison in vitro and in vivo |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079117/ https://www.ncbi.nlm.nih.gov/pubmed/35542822 http://dx.doi.org/10.1039/c7ra13760e |
work_keys_str_mv | AT zhangxia identificationofmetabolitesofliquiritininratsbyuhplcqtofmsmsmetabolicprofilingandpathwaycomparisoninvitroandinvivo AT liangcaijuan identificationofmetabolitesofliquiritininratsbyuhplcqtofmsmsmetabolicprofilingandpathwaycomparisoninvitroandinvivo AT yinjintuo identificationofmetabolitesofliquiritininratsbyuhplcqtofmsmsmetabolicprofilingandpathwaycomparisoninvitroandinvivo AT sunyupeng identificationofmetabolitesofliquiritininratsbyuhplcqtofmsmsmetabolicprofilingandpathwaycomparisoninvitroandinvivo AT zhanglantong identificationofmetabolitesofliquiritininratsbyuhplcqtofmsmsmetabolicprofilingandpathwaycomparisoninvitroandinvivo |