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“Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex
Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079255/ https://www.ncbi.nlm.nih.gov/pubmed/35542809 http://dx.doi.org/10.1039/c7ra13404e |
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author | Wang, Miao Li, Minmin Lu, Jia Fan, Bei He, Yan Huang, Yatao Wang, Fengzhong |
author_facet | Wang, Miao Li, Minmin Lu, Jia Fan, Bei He, Yan Huang, Yatao Wang, Fengzhong |
author_sort | Wang, Miao |
collection | PubMed |
description | Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-catalyzed hydrolysis of acetylthiocholine (ATCh) and effectively scavenges Au(iii). In the presence of organophosphates, the catalytic activity of AChE is inhibited, allowing these species to be quantified based on the decreased recovery of CD fluorescence intensity. The developed sensor was used to analyze a real pesticide-spiked sample (4.48 μM), achieving a recovery of 99.85% and exhibiting a linear response range of 0.45–44.80 μM. |
format | Online Article Text |
id | pubmed-9079255 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90792552022-05-09 “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex Wang, Miao Li, Minmin Lu, Jia Fan, Bei He, Yan Huang, Yatao Wang, Fengzhong RSC Adv Chemistry Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-catalyzed hydrolysis of acetylthiocholine (ATCh) and effectively scavenges Au(iii). In the presence of organophosphates, the catalytic activity of AChE is inhibited, allowing these species to be quantified based on the decreased recovery of CD fluorescence intensity. The developed sensor was used to analyze a real pesticide-spiked sample (4.48 μM), achieving a recovery of 99.85% and exhibiting a linear response range of 0.45–44.80 μM. The Royal Society of Chemistry 2018-03-23 /pmc/articles/PMC9079255/ /pubmed/35542809 http://dx.doi.org/10.1039/c7ra13404e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Wang, Miao Li, Minmin Lu, Jia Fan, Bei He, Yan Huang, Yatao Wang, Fengzhong “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex |
title | “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex |
title_full | “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex |
title_fullStr | “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex |
title_full_unstemmed | “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex |
title_short | “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex |
title_sort | “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot–au(iii) complex |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079255/ https://www.ncbi.nlm.nih.gov/pubmed/35542809 http://dx.doi.org/10.1039/c7ra13404e |
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