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“Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex

Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-...

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Detalles Bibliográficos
Autores principales: Wang, Miao, Li, Minmin, Lu, Jia, Fan, Bei, He, Yan, Huang, Yatao, Wang, Fengzhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079255/
https://www.ncbi.nlm.nih.gov/pubmed/35542809
http://dx.doi.org/10.1039/c7ra13404e
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author Wang, Miao
Li, Minmin
Lu, Jia
Fan, Bei
He, Yan
Huang, Yatao
Wang, Fengzhong
author_facet Wang, Miao
Li, Minmin
Lu, Jia
Fan, Bei
He, Yan
Huang, Yatao
Wang, Fengzhong
author_sort Wang, Miao
collection PubMed
description Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-catalyzed hydrolysis of acetylthiocholine (ATCh) and effectively scavenges Au(iii). In the presence of organophosphates, the catalytic activity of AChE is inhibited, allowing these species to be quantified based on the decreased recovery of CD fluorescence intensity. The developed sensor was used to analyze a real pesticide-spiked sample (4.48 μM), achieving a recovery of 99.85% and exhibiting a linear response range of 0.45–44.80 μM.
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spelling pubmed-90792552022-05-09 “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex Wang, Miao Li, Minmin Lu, Jia Fan, Bei He, Yan Huang, Yatao Wang, Fengzhong RSC Adv Chemistry Herein, we report novel “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot (CD)–Au(iii) complex/acetylcholinesterase (AChE) system. The above sensor utilizes the quenching of CD fluorescence by Au(iii) and its subsequent recovery by thiocholine, which is generated by AChE-catalyzed hydrolysis of acetylthiocholine (ATCh) and effectively scavenges Au(iii). In the presence of organophosphates, the catalytic activity of AChE is inhibited, allowing these species to be quantified based on the decreased recovery of CD fluorescence intensity. The developed sensor was used to analyze a real pesticide-spiked sample (4.48 μM), achieving a recovery of 99.85% and exhibiting a linear response range of 0.45–44.80 μM. The Royal Society of Chemistry 2018-03-23 /pmc/articles/PMC9079255/ /pubmed/35542809 http://dx.doi.org/10.1039/c7ra13404e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Wang, Miao
Li, Minmin
Lu, Jia
Fan, Bei
He, Yan
Huang, Yatao
Wang, Fengzhong
“Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex
title “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex
title_full “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex
title_fullStr “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex
title_full_unstemmed “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex
title_short “Off–On” fluorescent sensing of organophosphate pesticides using a carbon dot–Au(iii) complex
title_sort “off–on” fluorescent sensing of organophosphate pesticides using a carbon dot–au(iii) complex
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079255/
https://www.ncbi.nlm.nih.gov/pubmed/35542809
http://dx.doi.org/10.1039/c7ra13404e
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