DNA-induced synthesis of biomimetic enzyme for sensitive detection of superoxide anions released from live cell

In this work, we successfully fabricate a rapid, sensitive sensor for the detection of superoxide anions O(2)˙(−) based on graphene/DNA/Mn(3)(PO(4))(2) biomimetic enzyme. In the design, graphene is served as excellent carrier to improve the catalysis of Mn(3)(PO(4))(2) nanoparticles; and DNA adsorbe...

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Detalles Bibliográficos
Autores principales: Ding, Ailing, Wang, Bin, Ma, Xiaoqing, Diao, Jianglin, Zheng, Jiushang, Chen, Jiucun, Li, Changming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079267/
https://www.ncbi.nlm.nih.gov/pubmed/35539377
http://dx.doi.org/10.1039/c7ra12962a
Descripción
Sumario:In this work, we successfully fabricate a rapid, sensitive sensor for the detection of superoxide anions O(2)˙(−) based on graphene/DNA/Mn(3)(PO(4))(2) biomimetic enzyme. In the design, graphene is served as excellent carrier to improve the catalysis of Mn(3)(PO(4))(2) nanoparticles; and DNA adsorbed on graphene acts as medium to assist the growth of Mn(3)(PO(4))(2) on graphene. The fabricated graphene/DNA/Mn(3)(PO(4))(2) composites exhibit excellently electrochemical activity, significantly decrease the response time and increase the sensitivity of the sensor towards O(2)˙(−). The successful detection of O(2)˙(−) released from cancer cell demonstrated its potential applications in biology and medicine.

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