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Protocol for fluorescence-activated cell sorting of human EpCAM(+) lung cancer cells for gene expression analysis of Rac guanine-nucleotide exchange factors

Here, we describe a protocol for fluorescence-activated cell sorting (FACS) of human EpCAM(+) cells from fresh surgically resected specimens. We then use Q-PCR to identify specific molecular targets associated with the metastatic phenotype. This combined approach enables a qualitative and quantitati...

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Detalles Bibliográficos
Autores principales:	Sullivan, Neil T., Cooke, Mariana
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079335/ https://www.ncbi.nlm.nih.gov/pubmed/35542175 http://dx.doi.org/10.1016/j.xpro.2022.101367

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