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Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy

Epithelial cell adhesion molecules (EpCAMs) play a significant role in tumorigenesis and tumor development. EpCAMs are considered to be tumor signaling molecules for cancer diagnosis, prognosis and therapy. Herein, an enzyme-free and highly sensitive fluorescent biosensor, with a combined aptamer-ba...

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Autores principales: Chen, Jishun, Shang, Bing, Zhang, Hua, Zhu, Zhengpeng, Chen, Long, Wang, Hongmei, Ran, Fengying, Chen, Qinhua, Chen, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079946/
https://www.ncbi.nlm.nih.gov/pubmed/35541343
http://dx.doi.org/10.1039/c8ra01362d
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author Chen, Jishun
Shang, Bing
Zhang, Hua
Zhu, Zhengpeng
Chen, Long
Wang, Hongmei
Ran, Fengying
Chen, Qinhua
Chen, Jun
author_facet Chen, Jishun
Shang, Bing
Zhang, Hua
Zhu, Zhengpeng
Chen, Long
Wang, Hongmei
Ran, Fengying
Chen, Qinhua
Chen, Jun
author_sort Chen, Jishun
collection PubMed
description Epithelial cell adhesion molecules (EpCAMs) play a significant role in tumorigenesis and tumor development. EpCAMs are considered to be tumor signaling molecules for cancer diagnosis, prognosis and therapy. Herein, an enzyme-free and highly sensitive fluorescent biosensor, with a combined aptamer-based EpCAM recognition and toehold-aided DNA recycling amplification strategy, was developed for sensitive and specific fluorescence detection of EpCAMs. Due to highly specific binding between EpCAMs and corresponding aptamers, strand a, which is released from the complex of aptamer/strand a in the presence of EpCAMs which is bound to the corresponding aptamer, triggered the toehold-mediated strand displacement process. An amplified fluorescent signal was achieved by recycling strand a for ultrasensitive EpCAM detection with a detection limit as low as 0.1 ng mL(−1), which was comparable or superior to that of reported immunoassays and biosensor strategies. In addition, high selectivity towards EpCAMs was exhibited when other proteins were selected as control proteins. Finally, this strategy was successfully used for the ultrasensitive fluorescence detection of EpCAMs in human serum samples with satisfactory results. Importantly, the present strategy may be also expanded for the detection of other targets using the corresponding aptamers.
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spelling pubmed-90799462022-05-09 Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy Chen, Jishun Shang, Bing Zhang, Hua Zhu, Zhengpeng Chen, Long Wang, Hongmei Ran, Fengying Chen, Qinhua Chen, Jun RSC Adv Chemistry Epithelial cell adhesion molecules (EpCAMs) play a significant role in tumorigenesis and tumor development. EpCAMs are considered to be tumor signaling molecules for cancer diagnosis, prognosis and therapy. Herein, an enzyme-free and highly sensitive fluorescent biosensor, with a combined aptamer-based EpCAM recognition and toehold-aided DNA recycling amplification strategy, was developed for sensitive and specific fluorescence detection of EpCAMs. Due to highly specific binding between EpCAMs and corresponding aptamers, strand a, which is released from the complex of aptamer/strand a in the presence of EpCAMs which is bound to the corresponding aptamer, triggered the toehold-mediated strand displacement process. An amplified fluorescent signal was achieved by recycling strand a for ultrasensitive EpCAM detection with a detection limit as low as 0.1 ng mL(−1), which was comparable or superior to that of reported immunoassays and biosensor strategies. In addition, high selectivity towards EpCAMs was exhibited when other proteins were selected as control proteins. Finally, this strategy was successfully used for the ultrasensitive fluorescence detection of EpCAMs in human serum samples with satisfactory results. Importantly, the present strategy may be also expanded for the detection of other targets using the corresponding aptamers. The Royal Society of Chemistry 2018-04-19 /pmc/articles/PMC9079946/ /pubmed/35541343 http://dx.doi.org/10.1039/c8ra01362d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Chen, Jishun
Shang, Bing
Zhang, Hua
Zhu, Zhengpeng
Chen, Long
Wang, Hongmei
Ran, Fengying
Chen, Qinhua
Chen, Jun
Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy
title Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy
title_full Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy
title_fullStr Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy
title_full_unstemmed Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy
title_short Enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided DNA recycling amplification strategy
title_sort enzyme-free ultrasensitive fluorescence detection of epithelial cell adhesion molecules based on a toehold-aided dna recycling amplification strategy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079946/
https://www.ncbi.nlm.nih.gov/pubmed/35541343
http://dx.doi.org/10.1039/c8ra01362d
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