Cargando…
Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA
In a biological microenvironment, biological macromolecules could interact with nanoparticles (NPs) and consequently influence the toxicity of NPs. This study investigated the effects of BSA or palmitic acids complexed to BSA (PA–BSA) on the toxicity of ZnO NPs to THP-1 macrophages. Atomic force mic...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079995/ https://www.ncbi.nlm.nih.gov/pubmed/35539503 http://dx.doi.org/10.1039/c8ra02509f |
_version_ | 1784702683536424960 |
---|---|
author | Gong, Yu Li, Xianqiang Liao, Guochao Ding, Yanhuai Li, Juan Cao, Yi |
author_facet | Gong, Yu Li, Xianqiang Liao, Guochao Ding, Yanhuai Li, Juan Cao, Yi |
author_sort | Gong, Yu |
collection | PubMed |
description | In a biological microenvironment, biological macromolecules could interact with nanoparticles (NPs) and consequently influence the toxicity of NPs. This study investigated the effects of BSA or palmitic acids complexed to BSA (PA–BSA) on the toxicity of ZnO NPs to THP-1 macrophages. Atomic force microscopy showed the increase of NP heights after pre-incubation with BSA or PA–BSA, but PA–BSA more effectively altered the hydrodynamic size and zeta potential of NPs. Pre-incubation with BSA but not PA–BSA alleviated ZnO NP induced cytotoxicity, and transmission electron microscopy confirmed fewer intrastructural changes after exposure to ZnO NPs pre-incubated with BSA. ZnO NP exposure increased intracellular Zn ions but decreased reactive oxygen species (ROS) and release of soluble monocyte chemotactic protein-1 (sMCP-1), whereas pre-incubation with BSA and PA–BSA induced a different pattern of intracellular Zn ions and modestly increased intracellular ROS. The expression of ER stress marker DDIT3 was only significantly induced after exposure to NPs pre-incubated with PA–BSA, and CASP12 expression was significantly lower after exposure to NPs pre-incubated with BSA compared to NPs with or without pre-incubation of PA–BSA. In summary, these results showed that pre-incubation with BSA was more effective compared with PA–BSA to alleviate the toxicity of ZnO NPs to THP-1 macrophages, which should be considered for the evaluation of NP toxicity in a biological microenvironment. |
format | Online Article Text |
id | pubmed-9079995 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90799952022-05-09 Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA Gong, Yu Li, Xianqiang Liao, Guochao Ding, Yanhuai Li, Juan Cao, Yi RSC Adv Chemistry In a biological microenvironment, biological macromolecules could interact with nanoparticles (NPs) and consequently influence the toxicity of NPs. This study investigated the effects of BSA or palmitic acids complexed to BSA (PA–BSA) on the toxicity of ZnO NPs to THP-1 macrophages. Atomic force microscopy showed the increase of NP heights after pre-incubation with BSA or PA–BSA, but PA–BSA more effectively altered the hydrodynamic size and zeta potential of NPs. Pre-incubation with BSA but not PA–BSA alleviated ZnO NP induced cytotoxicity, and transmission electron microscopy confirmed fewer intrastructural changes after exposure to ZnO NPs pre-incubated with BSA. ZnO NP exposure increased intracellular Zn ions but decreased reactive oxygen species (ROS) and release of soluble monocyte chemotactic protein-1 (sMCP-1), whereas pre-incubation with BSA and PA–BSA induced a different pattern of intracellular Zn ions and modestly increased intracellular ROS. The expression of ER stress marker DDIT3 was only significantly induced after exposure to NPs pre-incubated with PA–BSA, and CASP12 expression was significantly lower after exposure to NPs pre-incubated with BSA compared to NPs with or without pre-incubation of PA–BSA. In summary, these results showed that pre-incubation with BSA was more effective compared with PA–BSA to alleviate the toxicity of ZnO NPs to THP-1 macrophages, which should be considered for the evaluation of NP toxicity in a biological microenvironment. The Royal Society of Chemistry 2018-04-24 /pmc/articles/PMC9079995/ /pubmed/35539503 http://dx.doi.org/10.1039/c8ra02509f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/ |
spellingShingle | Chemistry Gong, Yu Li, Xianqiang Liao, Guochao Ding, Yanhuai Li, Juan Cao, Yi Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA |
title | Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA |
title_full | Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA |
title_fullStr | Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA |
title_full_unstemmed | Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA |
title_short | Cytotoxicity and ER stress–apoptosis gene expression in ZnO nanoparticle exposed THP-1 macrophages: influence of pre-incubation with BSA or palmitic acids complexed to BSA |
title_sort | cytotoxicity and er stress–apoptosis gene expression in zno nanoparticle exposed thp-1 macrophages: influence of pre-incubation with bsa or palmitic acids complexed to bsa |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9079995/ https://www.ncbi.nlm.nih.gov/pubmed/35539503 http://dx.doi.org/10.1039/c8ra02509f |
work_keys_str_mv | AT gongyu cytotoxicityanderstressapoptosisgeneexpressioninznonanoparticleexposedthp1macrophagesinfluenceofpreincubationwithbsaorpalmiticacidscomplexedtobsa AT lixianqiang cytotoxicityanderstressapoptosisgeneexpressioninznonanoparticleexposedthp1macrophagesinfluenceofpreincubationwithbsaorpalmiticacidscomplexedtobsa AT liaoguochao cytotoxicityanderstressapoptosisgeneexpressioninznonanoparticleexposedthp1macrophagesinfluenceofpreincubationwithbsaorpalmiticacidscomplexedtobsa AT dingyanhuai cytotoxicityanderstressapoptosisgeneexpressioninznonanoparticleexposedthp1macrophagesinfluenceofpreincubationwithbsaorpalmiticacidscomplexedtobsa AT lijuan cytotoxicityanderstressapoptosisgeneexpressioninznonanoparticleexposedthp1macrophagesinfluenceofpreincubationwithbsaorpalmiticacidscomplexedtobsa AT caoyi cytotoxicityanderstressapoptosisgeneexpressioninznonanoparticleexposedthp1macrophagesinfluenceofpreincubationwithbsaorpalmiticacidscomplexedtobsa |