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Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method

The comprehensive characterization of metabolome and lipidome to reveal unknown pathological conditions, are being used to investigate the molecular mechanisms of cancer, especially in the field of early diagnosis, treatment, and prognosis. The multidrug resistance (MDR) of tumor cells limits the th...

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Detalles Bibliográficos
Autores principales: Zong, Li, Pi, Zifeng, Liu, Shu, Liu, Zhiqiang, Song, Fengrui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080077/
https://www.ncbi.nlm.nih.gov/pubmed/35539507
http://dx.doi.org/10.1039/c7ra12952a
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author Zong, Li
Pi, Zifeng
Liu, Shu
Liu, Zhiqiang
Song, Fengrui
author_facet Zong, Li
Pi, Zifeng
Liu, Shu
Liu, Zhiqiang
Song, Fengrui
author_sort Zong, Li
collection PubMed
description The comprehensive characterization of metabolome and lipidome to reveal unknown pathological conditions, are being used to investigate the molecular mechanisms of cancer, especially in the field of early diagnosis, treatment, and prognosis. The multidrug resistance (MDR) of tumor cells limits the therapeutic effect of anti-cancer drugs and is the main obstacle for chemotherapy. Here, we adopted a methyl-tert-butyl ether (MTBE)-based extraction method to simultaneously extract small polar molecules and lipophilic metabolites for nontargeted metabolomics of multidrug-resistant breast cancer cell line MCF-7/ADR and its parental cell line MCF-7/S by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Distinctly different metabolic features were shown between MCF-7/ADR cells and MCF-7/S on the basis of multivariate analyses. 17 potential biomarkers were identified. And these potential biomarkers were mainly correlated with cell membrane lipids composition, cell signaling regulated by lipids, and anti-oxidation ability. The studies of cellular ultrastructure and morphology by in situ atomic force microscopy (AFM) also demonstrated the cellular membrane changed along with the MDR. We expect that this study could provide a new method for monitoring drug resistance during clinical chemotherapy and be useful for the development of drugs to overcome the MDR.
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spelling pubmed-90800772022-05-09 Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method Zong, Li Pi, Zifeng Liu, Shu Liu, Zhiqiang Song, Fengrui RSC Adv Chemistry The comprehensive characterization of metabolome and lipidome to reveal unknown pathological conditions, are being used to investigate the molecular mechanisms of cancer, especially in the field of early diagnosis, treatment, and prognosis. The multidrug resistance (MDR) of tumor cells limits the therapeutic effect of anti-cancer drugs and is the main obstacle for chemotherapy. Here, we adopted a methyl-tert-butyl ether (MTBE)-based extraction method to simultaneously extract small polar molecules and lipophilic metabolites for nontargeted metabolomics of multidrug-resistant breast cancer cell line MCF-7/ADR and its parental cell line MCF-7/S by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry. Distinctly different metabolic features were shown between MCF-7/ADR cells and MCF-7/S on the basis of multivariate analyses. 17 potential biomarkers were identified. And these potential biomarkers were mainly correlated with cell membrane lipids composition, cell signaling regulated by lipids, and anti-oxidation ability. The studies of cellular ultrastructure and morphology by in situ atomic force microscopy (AFM) also demonstrated the cellular membrane changed along with the MDR. We expect that this study could provide a new method for monitoring drug resistance during clinical chemotherapy and be useful for the development of drugs to overcome the MDR. The Royal Society of Chemistry 2018-04-26 /pmc/articles/PMC9080077/ /pubmed/35539507 http://dx.doi.org/10.1039/c7ra12952a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Zong, Li
Pi, Zifeng
Liu, Shu
Liu, Zhiqiang
Song, Fengrui
Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method
title Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method
title_full Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method
title_fullStr Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method
title_full_unstemmed Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method
title_short Metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method
title_sort metabolomics analysis of multidrug-resistant breast cancer cells in vitro using methyl-tert-butyl ether method
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080077/
https://www.ncbi.nlm.nih.gov/pubmed/35539507
http://dx.doi.org/10.1039/c7ra12952a
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