Cargando…
Acute bone damage through liver-bone axis induced by thioacetamide in rats
BACKGROUND: Thioacetamide (TAA) is used in various fields, such as synthetic drugs, organic chemical synthesis, and materials chemistry. TAA is mainly used to establish animal liver injury models and other organ damage models to explore their mechanisms for helping patients with liver disease. Liver...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080193/ https://www.ncbi.nlm.nih.gov/pubmed/35526079 http://dx.doi.org/10.1186/s40360-022-00568-4 |
_version_ | 1784702728763604992 |
---|---|
author | Jin, Xiaoli Li, Yang Li, Jianghua Cheng, Linyan Yao, Yetao Shen, Hao Wang, Bili Ren, Jun Ying, Hang Xu, Jian |
author_facet | Jin, Xiaoli Li, Yang Li, Jianghua Cheng, Linyan Yao, Yetao Shen, Hao Wang, Bili Ren, Jun Ying, Hang Xu, Jian |
author_sort | Jin, Xiaoli |
collection | PubMed |
description | BACKGROUND: Thioacetamide (TAA) is used in various fields, such as synthetic drugs, organic chemical synthesis, and materials chemistry. TAA is mainly used to establish animal liver injury models and other organ damage models to explore their mechanisms for helping patients with liver disease. Liver damage can lead to abnormal expression of some enzymes in the serum, so we detected the appropriate enzyme levels in the serum of SD rats to verify the damage of TAA to the liver. More importantly, TAA caused bone damage is barely understood. Therefore, our research aims to establish a rat model reflecting the acute bone damage injury caused by TAA. METHODS: The SD rats were intraperitoneally injected with normal saline (0.9%) or TAA (200 mg/kg, 400 mg/kg) for 1 month (once the other day). After the last intraperitoneal injection, serum samples from rats were used for biochemical tests. Masson staining is used to detect liver damage, and micro-CT is used to detect the changes in bone. Moreover, the three-point bending experiment was used to detect the force range of the hind limbs of SD rats. RESULTS: Compared with the control group, after the intraperitoneal injection of TAA, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA), total bile acid (TBA), alkaline phosphatase (ALP), carbamide (UREA) and creatinine (CREA) rose sharply, while the levels of serum content of total protein (TP), lactate dehydrogenase (LDH), calcium (Ca) and phosphorus (P) were severely reduced. After TAA administration, collagen fibers were deposited and liver fibrosis was obvious. Micro-CT results showed that the bone surface, tissue surface, bone volume, and tissue volume of rats with an intraperitoneal injection of TAA were significantly reduced. In addition, the bones of rats with an intraperitoneal injection of TAA can resist less pressure and are prone to fractures. CONCLUSIONS: TAA can cause liver damage in SD rats, which is explained by the changes in serum biochemical indicators and the deposition of liver collagen. More importantly, TAA can reduce bone mineral density and increase the separation of bone trabeculae in SD rats, and finally lead to bone injury. This suggests that TAA may become an ideal model to investigate abnormal bone metabolism after liver injury. |
format | Online Article Text |
id | pubmed-9080193 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-90801932022-05-09 Acute bone damage through liver-bone axis induced by thioacetamide in rats Jin, Xiaoli Li, Yang Li, Jianghua Cheng, Linyan Yao, Yetao Shen, Hao Wang, Bili Ren, Jun Ying, Hang Xu, Jian BMC Pharmacol Toxicol Research Article BACKGROUND: Thioacetamide (TAA) is used in various fields, such as synthetic drugs, organic chemical synthesis, and materials chemistry. TAA is mainly used to establish animal liver injury models and other organ damage models to explore their mechanisms for helping patients with liver disease. Liver damage can lead to abnormal expression of some enzymes in the serum, so we detected the appropriate enzyme levels in the serum of SD rats to verify the damage of TAA to the liver. More importantly, TAA caused bone damage is barely understood. Therefore, our research aims to establish a rat model reflecting the acute bone damage injury caused by TAA. METHODS: The SD rats were intraperitoneally injected with normal saline (0.9%) or TAA (200 mg/kg, 400 mg/kg) for 1 month (once the other day). After the last intraperitoneal injection, serum samples from rats were used for biochemical tests. Masson staining is used to detect liver damage, and micro-CT is used to detect the changes in bone. Moreover, the three-point bending experiment was used to detect the force range of the hind limbs of SD rats. RESULTS: Compared with the control group, after the intraperitoneal injection of TAA, the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), uric acid (UA), total bile acid (TBA), alkaline phosphatase (ALP), carbamide (UREA) and creatinine (CREA) rose sharply, while the levels of serum content of total protein (TP), lactate dehydrogenase (LDH), calcium (Ca) and phosphorus (P) were severely reduced. After TAA administration, collagen fibers were deposited and liver fibrosis was obvious. Micro-CT results showed that the bone surface, tissue surface, bone volume, and tissue volume of rats with an intraperitoneal injection of TAA were significantly reduced. In addition, the bones of rats with an intraperitoneal injection of TAA can resist less pressure and are prone to fractures. CONCLUSIONS: TAA can cause liver damage in SD rats, which is explained by the changes in serum biochemical indicators and the deposition of liver collagen. More importantly, TAA can reduce bone mineral density and increase the separation of bone trabeculae in SD rats, and finally lead to bone injury. This suggests that TAA may become an ideal model to investigate abnormal bone metabolism after liver injury. BioMed Central 2022-05-07 /pmc/articles/PMC9080193/ /pubmed/35526079 http://dx.doi.org/10.1186/s40360-022-00568-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Jin, Xiaoli Li, Yang Li, Jianghua Cheng, Linyan Yao, Yetao Shen, Hao Wang, Bili Ren, Jun Ying, Hang Xu, Jian Acute bone damage through liver-bone axis induced by thioacetamide in rats |
title | Acute bone damage through liver-bone axis induced by thioacetamide in rats |
title_full | Acute bone damage through liver-bone axis induced by thioacetamide in rats |
title_fullStr | Acute bone damage through liver-bone axis induced by thioacetamide in rats |
title_full_unstemmed | Acute bone damage through liver-bone axis induced by thioacetamide in rats |
title_short | Acute bone damage through liver-bone axis induced by thioacetamide in rats |
title_sort | acute bone damage through liver-bone axis induced by thioacetamide in rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080193/ https://www.ncbi.nlm.nih.gov/pubmed/35526079 http://dx.doi.org/10.1186/s40360-022-00568-4 |
work_keys_str_mv | AT jinxiaoli acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT liyang acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT lijianghua acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT chenglinyan acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT yaoyetao acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT shenhao acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT wangbili acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT renjun acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT yinghang acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats AT xujian acutebonedamagethroughliverboneaxisinducedbythioacetamideinrats |