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A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C

A multi-functional anti-pathogen coating with “release-killing”, “contact-killing” and “anti-adhesion” properties was prepared from biocompatible polymer encapsulated chlorine dioxide (ClO(2)) which protected the active ingredient from the outside environment. A slow sustained-release of ClO(2) from...

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Autores principales: Li, Yan, Pi, Qing-meng, You, Hui-hui, Li, Jin-quan, Wang, Peng-cheng, Yang, Xu, Wu, Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080516/
https://www.ncbi.nlm.nih.gov/pubmed/35541145
http://dx.doi.org/10.1039/c8ra01985a
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author Li, Yan
Pi, Qing-meng
You, Hui-hui
Li, Jin-quan
Wang, Peng-cheng
Yang, Xu
Wu, Yang
author_facet Li, Yan
Pi, Qing-meng
You, Hui-hui
Li, Jin-quan
Wang, Peng-cheng
Yang, Xu
Wu, Yang
author_sort Li, Yan
collection PubMed
description A multi-functional anti-pathogen coating with “release-killing”, “contact-killing” and “anti-adhesion” properties was prepared from biocompatible polymer encapsulated chlorine dioxide (ClO(2)) which protected the active ingredient from the outside environment. A slow sustained-release of ClO(2) from micelles over fifteen days was detected for long-term release-killing. Micelles only release ClO(2) on demand in minimum inhibitory concentrations. We prepared nanoparticles which were covalently clustered on micelle surfaces to improve contact-killing as well as to improve the stability of the micelle. Copper nanoparticles were generated using the biosynthesis method including l-vitamin C, which avoids the toxicity and allows for the preparation of copper nanoparticles in a green environment. Synergistic anti-pathogen activity could be generated by a combination of micelle released ClO(2) and ascorbic acid. In addition to release-killing and contact-killing, a pluronic polymer coated surface also provides an additional “anti-adhesion” property through its protein-repelling ability. In this research, the designed coating demonstrated a broad-spectrum of activity to kill drug-resistant bacteria, viruses and spores in short period of time. Based on scanning electron microscopy (SEM), transmission electron microscopy (TEM) and anti-oxidase assays, we found that the designed coatings killed the pathogens via bio-oxidation. We also carried out acute respiratory toxicity tests in this research. Analysis of blood samples, lung function and histopathological slices indicated that the synthesized micelles allowed a controlled and sustained release of ClO(2) to kill pathogens while maintaining an overall ClO(2) concentration in the air within a safe range.
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spelling pubmed-90805162022-05-09 A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C Li, Yan Pi, Qing-meng You, Hui-hui Li, Jin-quan Wang, Peng-cheng Yang, Xu Wu, Yang RSC Adv Chemistry A multi-functional anti-pathogen coating with “release-killing”, “contact-killing” and “anti-adhesion” properties was prepared from biocompatible polymer encapsulated chlorine dioxide (ClO(2)) which protected the active ingredient from the outside environment. A slow sustained-release of ClO(2) from micelles over fifteen days was detected for long-term release-killing. Micelles only release ClO(2) on demand in minimum inhibitory concentrations. We prepared nanoparticles which were covalently clustered on micelle surfaces to improve contact-killing as well as to improve the stability of the micelle. Copper nanoparticles were generated using the biosynthesis method including l-vitamin C, which avoids the toxicity and allows for the preparation of copper nanoparticles in a green environment. Synergistic anti-pathogen activity could be generated by a combination of micelle released ClO(2) and ascorbic acid. In addition to release-killing and contact-killing, a pluronic polymer coated surface also provides an additional “anti-adhesion” property through its protein-repelling ability. In this research, the designed coating demonstrated a broad-spectrum of activity to kill drug-resistant bacteria, viruses and spores in short period of time. Based on scanning electron microscopy (SEM), transmission electron microscopy (TEM) and anti-oxidase assays, we found that the designed coatings killed the pathogens via bio-oxidation. We also carried out acute respiratory toxicity tests in this research. Analysis of blood samples, lung function and histopathological slices indicated that the synthesized micelles allowed a controlled and sustained release of ClO(2) to kill pathogens while maintaining an overall ClO(2) concentration in the air within a safe range. The Royal Society of Chemistry 2018-05-18 /pmc/articles/PMC9080516/ /pubmed/35541145 http://dx.doi.org/10.1039/c8ra01985a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Li, Yan
Pi, Qing-meng
You, Hui-hui
Li, Jin-quan
Wang, Peng-cheng
Yang, Xu
Wu, Yang
A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C
title A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C
title_full A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C
title_fullStr A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C
title_full_unstemmed A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C
title_short A smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin C
title_sort smart multi-functional coating based on anti-pathogen micelles tethered with copper nanoparticles via a biosynthesis method using l-vitamin c
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080516/
https://www.ncbi.nlm.nih.gov/pubmed/35541145
http://dx.doi.org/10.1039/c8ra01985a
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