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Novel dual-site fluorescent probe for monitoring cysteine and sulfite in living cells
Fluorescent probes have been considered to be efficient tools for the visualization of physiological and pathological processes. Herein, a dual-site fluorescence probe denoted as LC-1 was developed for the detection of cysteine (Cys) and its metabolite SO(3)(2−). The probe was shown to be highly sen...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080860/ https://www.ncbi.nlm.nih.gov/pubmed/35539911 http://dx.doi.org/10.1039/c8ra01683f |
Sumario: | Fluorescent probes have been considered to be efficient tools for the visualization of physiological and pathological processes. Herein, a dual-site fluorescence probe denoted as LC-1 was developed for the detection of cysteine (Cys) and its metabolite SO(3)(2−). The probe was shown to be highly sensitive to Cys and SO(3)(2−) with a turn-on mode fluorescence signal through two emission channels under excitations at wavelengths of 320 nm and 440 nm. Notably, the LC-1 probe was also observed to be satisfactorily sensitive to Cys and SO(3)(2−) in the presence of other amino acids and reactive oxygen species (ROS). Meanwhile, LC-1 was shown to have low cytotoxicity and was successfully applied for imaging the metabolism of Cys in living cells. |
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