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Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro

To regulate the behaviors and functions of endothelial cells (ECs) on the biomaterials on titanium (Ti), a biomimetic micropattern (ridge/groove: 25/25 μm) of polymer of 2-methacryloyloxyethyl phosphorylcholine (polyMPC) and Gly-Arg-Glu-Asp-Val-Tyr (GREDVY) was fabricated. PMMPC (monomer contain MPC...

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Autores principales: Ma, Wenyong, Liu, Luying, Chen, Huiqing, Zhao, Yuancong, Yang, Ping, Huang, Nan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080867/
https://www.ncbi.nlm.nih.gov/pubmed/35542362
http://dx.doi.org/10.1039/c8ra02607f
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author Ma, Wenyong
Liu, Luying
Chen, Huiqing
Zhao, Yuancong
Yang, Ping
Huang, Nan
author_facet Ma, Wenyong
Liu, Luying
Chen, Huiqing
Zhao, Yuancong
Yang, Ping
Huang, Nan
author_sort Ma, Wenyong
collection PubMed
description To regulate the behaviors and functions of endothelial cells (ECs) on the biomaterials on titanium (Ti), a biomimetic micropattern (ridge/groove: 25/25 μm) of polymer of 2-methacryloyloxyethyl phosphorylcholine (polyMPC) and Gly-Arg-Glu-Asp-Val-Tyr (GREDVY) was fabricated. PMMPC (monomer contain MPC and methacrylic acid (MA)) containing carboxyl groups was chosen, and PMMPC was cross-linked with hexamethylene diamine through condensation reaction of amino and carboxyl. Simultaneously, the carboxyl groups of cross-linked PMMPC (PMMPC-HD) can react with amino groups of polydopamine which can adhered on many materials firmly. GREDVY was immobilized on polydopamine but not on PMMPC-HD because amino and carboxyl groups can react with catechol and amino groups of polydopamine. IR and (1)H NMR demonstrated that PMMPC-HD was successfully synthesized. And the QCM-D (quartz crystal microbalance with dissipation) and IR approved that PMMPC-HD and GREDVY can be immobilized on polydopamine (PDA). Platelet adhesion and whole blood adhesion on micropattern modificated with PMMPC and GREDVY (Ti-PDA-M/R(P)) showed better hemocompatibility than other samples. Endothelial cells were regulated in the direction of micropattern showing elongated ECs were closer to a healthy, athero-protective phenotype than ECs cultured in vitro without micropattern. NO and PGI(2) release were upregulated. Simultaneously the number of SMCs on Ti-PDA-M/R(P) was the smaller that of other samples, which demonstrated that the Ti-PDA-M/R(P) had property of inhibiting SMCs proliferation to a certain extent.
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spelling pubmed-90808672022-05-09 Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro Ma, Wenyong Liu, Luying Chen, Huiqing Zhao, Yuancong Yang, Ping Huang, Nan RSC Adv Chemistry To regulate the behaviors and functions of endothelial cells (ECs) on the biomaterials on titanium (Ti), a biomimetic micropattern (ridge/groove: 25/25 μm) of polymer of 2-methacryloyloxyethyl phosphorylcholine (polyMPC) and Gly-Arg-Glu-Asp-Val-Tyr (GREDVY) was fabricated. PMMPC (monomer contain MPC and methacrylic acid (MA)) containing carboxyl groups was chosen, and PMMPC was cross-linked with hexamethylene diamine through condensation reaction of amino and carboxyl. Simultaneously, the carboxyl groups of cross-linked PMMPC (PMMPC-HD) can react with amino groups of polydopamine which can adhered on many materials firmly. GREDVY was immobilized on polydopamine but not on PMMPC-HD because amino and carboxyl groups can react with catechol and amino groups of polydopamine. IR and (1)H NMR demonstrated that PMMPC-HD was successfully synthesized. And the QCM-D (quartz crystal microbalance with dissipation) and IR approved that PMMPC-HD and GREDVY can be immobilized on polydopamine (PDA). Platelet adhesion and whole blood adhesion on micropattern modificated with PMMPC and GREDVY (Ti-PDA-M/R(P)) showed better hemocompatibility than other samples. Endothelial cells were regulated in the direction of micropattern showing elongated ECs were closer to a healthy, athero-protective phenotype than ECs cultured in vitro without micropattern. NO and PGI(2) release were upregulated. Simultaneously the number of SMCs on Ti-PDA-M/R(P) was the smaller that of other samples, which demonstrated that the Ti-PDA-M/R(P) had property of inhibiting SMCs proliferation to a certain extent. The Royal Society of Chemistry 2018-06-06 /pmc/articles/PMC9080867/ /pubmed/35542362 http://dx.doi.org/10.1039/c8ra02607f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Ma, Wenyong
Liu, Luying
Chen, Huiqing
Zhao, Yuancong
Yang, Ping
Huang, Nan
Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro
title Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro
title_full Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro
title_fullStr Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro
title_full_unstemmed Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro
title_short Micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro
title_sort micropatterned immobilization of membrane-mimicking polymer and peptides for regulation of cell behaviors in vitro
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9080867/
https://www.ncbi.nlm.nih.gov/pubmed/35542362
http://dx.doi.org/10.1039/c8ra02607f
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