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Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium

Stem cell–based therapy plays a pivotal role in the regeneration of damaged endometrium. Previous studies have demonstrated the therapeutic potential of bone marrow mesenchymal stem cells (BMSCs) through diverse administration ways. However, the homing, survival, and differentiation potential of the...

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Autores principales: Guo, Qi, Chang, Yajie, Li, Jingjie, Zhou, Chuanchuan, Huang, Rui, Yang, Xing, Liu, Guihua, Liang, Xiaoyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081369/
https://www.ncbi.nlm.nih.gov/pubmed/35547156
http://dx.doi.org/10.3389/fbioe.2022.735465
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author Guo, Qi
Chang, Yajie
Li, Jingjie
Zhou, Chuanchuan
Huang, Rui
Yang, Xing
Liu, Guihua
Liang, Xiaoyan
author_facet Guo, Qi
Chang, Yajie
Li, Jingjie
Zhou, Chuanchuan
Huang, Rui
Yang, Xing
Liu, Guihua
Liang, Xiaoyan
author_sort Guo, Qi
collection PubMed
description Stem cell–based therapy plays a pivotal role in the regeneration of damaged endometrium. Previous studies have demonstrated the therapeutic potential of bone marrow mesenchymal stem cells (BMSCs) through diverse administration ways. However, the homing, survival, and differentiation potential of these differently administered BMSCs are poorly defined, and the best route of administration is not well-defined. Herein, we aim to compare the engraftment, retaining time, and therapeutic efficiency of differently administered BMSCs. To achieve this, GFP/Luc-labeled BMSCs administered in two modes were assessed in a thin endometrium rat model: either into the damaged horns directly or through the ipsilateral iliac artery. The retaining time and hemi-quantitative distribution were evaluated by in vivo bioluminescence imaging and immunohistological analysis. Locally administered BMSCs were strongly detected in the abdomen at the first 4 days post treatment but underwent a rapid decrease in luminescent signal afterward and were rarely found 28 days after treatment. In contrast, the retaining time of BMSCs injected through the iliac artery was longer, reflected by more GFP-positive cells detected in the uterine section 28 days post treatment. Differentiation toward endometrial stromal cells was observed. Both routes of administration contributed to the restoration of the damaged endometrium, showing a comparable increase in the endometrial thickness and a decrease in fibrosis. However, more importantly, higher expression of LIF and VEGF, better recruitment, and longer retainment were found in the intra-arterial administration, contributing to the establishment of the optimal administration mode in clinical practice.
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spelling pubmed-90813692022-05-10 Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium Guo, Qi Chang, Yajie Li, Jingjie Zhou, Chuanchuan Huang, Rui Yang, Xing Liu, Guihua Liang, Xiaoyan Front Bioeng Biotechnol Bioengineering and Biotechnology Stem cell–based therapy plays a pivotal role in the regeneration of damaged endometrium. Previous studies have demonstrated the therapeutic potential of bone marrow mesenchymal stem cells (BMSCs) through diverse administration ways. However, the homing, survival, and differentiation potential of these differently administered BMSCs are poorly defined, and the best route of administration is not well-defined. Herein, we aim to compare the engraftment, retaining time, and therapeutic efficiency of differently administered BMSCs. To achieve this, GFP/Luc-labeled BMSCs administered in two modes were assessed in a thin endometrium rat model: either into the damaged horns directly or through the ipsilateral iliac artery. The retaining time and hemi-quantitative distribution were evaluated by in vivo bioluminescence imaging and immunohistological analysis. Locally administered BMSCs were strongly detected in the abdomen at the first 4 days post treatment but underwent a rapid decrease in luminescent signal afterward and were rarely found 28 days after treatment. In contrast, the retaining time of BMSCs injected through the iliac artery was longer, reflected by more GFP-positive cells detected in the uterine section 28 days post treatment. Differentiation toward endometrial stromal cells was observed. Both routes of administration contributed to the restoration of the damaged endometrium, showing a comparable increase in the endometrial thickness and a decrease in fibrosis. However, more importantly, higher expression of LIF and VEGF, better recruitment, and longer retainment were found in the intra-arterial administration, contributing to the establishment of the optimal administration mode in clinical practice. Frontiers Media S.A. 2022-04-25 /pmc/articles/PMC9081369/ /pubmed/35547156 http://dx.doi.org/10.3389/fbioe.2022.735465 Text en Copyright © 2022 Guo, Chang, Li, Zhou, Huang, Yang, Liu and Liang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Guo, Qi
Chang, Yajie
Li, Jingjie
Zhou, Chuanchuan
Huang, Rui
Yang, Xing
Liu, Guihua
Liang, Xiaoyan
Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium
title Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium
title_full Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium
title_fullStr Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium
title_full_unstemmed Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium
title_short Regenerative Effects of Locally or Intra-Arterially Administered BMSCs on the Thin Endometrium
title_sort regenerative effects of locally or intra-arterially administered bmscs on the thin endometrium
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081369/
https://www.ncbi.nlm.nih.gov/pubmed/35547156
http://dx.doi.org/10.3389/fbioe.2022.735465
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