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An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in bi...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Royal Society of Chemistry
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081577/ https://www.ncbi.nlm.nih.gov/pubmed/35540121 http://dx.doi.org/10.1039/c8ra02018c |
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author | Mishra, Priya Banga, Ivneet Tyagi, Roshika Munjal, Tanya Goel, Aditya Capalash, Neena Sharma, Prince Suri, C. R. Gandhi, Sonu |
author_facet | Mishra, Priya Banga, Ivneet Tyagi, Roshika Munjal, Tanya Goel, Aditya Capalash, Neena Sharma, Prince Suri, C. R. Gandhi, Sonu |
author_sort | Mishra, Priya |
collection | PubMed |
description | Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in biological urine samples. The presence of the drug was monitored after oral administration of heroin at different time intervals. Various biophysical techniques, such as high performance liquid chromatography (HPLC) and mass spectrometry (MS) were used to evaluate the presence of the drug. A competitive fluorescence based immunoassay was developed with a limit of detection (LOD) up to 0.01 ng mL(−1) and the IC(50) value was 0.1 ng mL(−1), while the dipstick assay shows a LOD up to 5 ng mL(−1). Rapid detection of narcotic drugs was carried out for biological urine samples collected at various time points. Validation of the developed dipstick was carried out for the standard as well as the spiked urine samples by fluorescence based immunoassay (FIA), using anti-morphine antibodies. A strong correlation (R = 0.94) was obtained between the developed dipstick and FIA assay for biological urine samples collected at various time points. The developed immunochromatographic dipstick is highly sensitive, field applicable and cost effective, and can serve as a first choice for the monitoring of narcotic drugs in blood, urine and saliva in drug addicts and athletes. |
format | Online Article Text |
id | pubmed-9081577 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-90815772022-05-09 An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples Mishra, Priya Banga, Ivneet Tyagi, Roshika Munjal, Tanya Goel, Aditya Capalash, Neena Sharma, Prince Suri, C. R. Gandhi, Sonu RSC Adv Chemistry Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in biological urine samples. The presence of the drug was monitored after oral administration of heroin at different time intervals. Various biophysical techniques, such as high performance liquid chromatography (HPLC) and mass spectrometry (MS) were used to evaluate the presence of the drug. A competitive fluorescence based immunoassay was developed with a limit of detection (LOD) up to 0.01 ng mL(−1) and the IC(50) value was 0.1 ng mL(−1), while the dipstick assay shows a LOD up to 5 ng mL(−1). Rapid detection of narcotic drugs was carried out for biological urine samples collected at various time points. Validation of the developed dipstick was carried out for the standard as well as the spiked urine samples by fluorescence based immunoassay (FIA), using anti-morphine antibodies. A strong correlation (R = 0.94) was obtained between the developed dipstick and FIA assay for biological urine samples collected at various time points. The developed immunochromatographic dipstick is highly sensitive, field applicable and cost effective, and can serve as a first choice for the monitoring of narcotic drugs in blood, urine and saliva in drug addicts and athletes. The Royal Society of Chemistry 2018-06-26 /pmc/articles/PMC9081577/ /pubmed/35540121 http://dx.doi.org/10.1039/c8ra02018c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Mishra, Priya Banga, Ivneet Tyagi, Roshika Munjal, Tanya Goel, Aditya Capalash, Neena Sharma, Prince Suri, C. R. Gandhi, Sonu An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples |
title | An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples |
title_full | An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples |
title_fullStr | An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples |
title_full_unstemmed | An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples |
title_short | An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples |
title_sort | immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081577/ https://www.ncbi.nlm.nih.gov/pubmed/35540121 http://dx.doi.org/10.1039/c8ra02018c |
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