Cargando…

An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples

Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in bi...

Descripción completa

Detalles Bibliográficos
Autores principales: Mishra, Priya, Banga, Ivneet, Tyagi, Roshika, Munjal, Tanya, Goel, Aditya, Capalash, Neena, Sharma, Prince, Suri, C. R., Gandhi, Sonu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081577/
https://www.ncbi.nlm.nih.gov/pubmed/35540121
http://dx.doi.org/10.1039/c8ra02018c
_version_ 1784703016476082176
author Mishra, Priya
Banga, Ivneet
Tyagi, Roshika
Munjal, Tanya
Goel, Aditya
Capalash, Neena
Sharma, Prince
Suri, C. R.
Gandhi, Sonu
author_facet Mishra, Priya
Banga, Ivneet
Tyagi, Roshika
Munjal, Tanya
Goel, Aditya
Capalash, Neena
Sharma, Prince
Suri, C. R.
Gandhi, Sonu
author_sort Mishra, Priya
collection PubMed
description Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in biological urine samples. The presence of the drug was monitored after oral administration of heroin at different time intervals. Various biophysical techniques, such as high performance liquid chromatography (HPLC) and mass spectrometry (MS) were used to evaluate the presence of the drug. A competitive fluorescence based immunoassay was developed with a limit of detection (LOD) up to 0.01 ng mL(−1) and the IC(50) value was 0.1 ng mL(−1), while the dipstick assay shows a LOD up to 5 ng mL(−1). Rapid detection of narcotic drugs was carried out for biological urine samples collected at various time points. Validation of the developed dipstick was carried out for the standard as well as the spiked urine samples by fluorescence based immunoassay (FIA), using anti-morphine antibodies. A strong correlation (R = 0.94) was obtained between the developed dipstick and FIA assay for biological urine samples collected at various time points. The developed immunochromatographic dipstick is highly sensitive, field applicable and cost effective, and can serve as a first choice for the monitoring of narcotic drugs in blood, urine and saliva in drug addicts and athletes.
format Online
Article
Text
id pubmed-9081577
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher The Royal Society of Chemistry
record_format MEDLINE/PubMed
spelling pubmed-90815772022-05-09 An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples Mishra, Priya Banga, Ivneet Tyagi, Roshika Munjal, Tanya Goel, Aditya Capalash, Neena Sharma, Prince Suri, C. R. Gandhi, Sonu RSC Adv Chemistry Heroin use and addiction pose serious risks and side effects due to overdose. Quantification of heroin in biological samples is challenging due to rapid deacetylation of heroin to its active metabolites. In this study, we report the quantification of metabolic degradation of heroin by-products in biological urine samples. The presence of the drug was monitored after oral administration of heroin at different time intervals. Various biophysical techniques, such as high performance liquid chromatography (HPLC) and mass spectrometry (MS) were used to evaluate the presence of the drug. A competitive fluorescence based immunoassay was developed with a limit of detection (LOD) up to 0.01 ng mL(−1) and the IC(50) value was 0.1 ng mL(−1), while the dipstick assay shows a LOD up to 5 ng mL(−1). Rapid detection of narcotic drugs was carried out for biological urine samples collected at various time points. Validation of the developed dipstick was carried out for the standard as well as the spiked urine samples by fluorescence based immunoassay (FIA), using anti-morphine antibodies. A strong correlation (R = 0.94) was obtained between the developed dipstick and FIA assay for biological urine samples collected at various time points. The developed immunochromatographic dipstick is highly sensitive, field applicable and cost effective, and can serve as a first choice for the monitoring of narcotic drugs in blood, urine and saliva in drug addicts and athletes. The Royal Society of Chemistry 2018-06-26 /pmc/articles/PMC9081577/ /pubmed/35540121 http://dx.doi.org/10.1039/c8ra02018c Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Mishra, Priya
Banga, Ivneet
Tyagi, Roshika
Munjal, Tanya
Goel, Aditya
Capalash, Neena
Sharma, Prince
Suri, C. R.
Gandhi, Sonu
An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
title An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
title_full An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
title_fullStr An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
title_full_unstemmed An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
title_short An immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
title_sort immunochromatographic dipstick as an alternate for monitoring of heroin metabolites in urine samples
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081577/
https://www.ncbi.nlm.nih.gov/pubmed/35540121
http://dx.doi.org/10.1039/c8ra02018c
work_keys_str_mv AT mishrapriya animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT bangaivneet animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT tyagiroshika animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT munjaltanya animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT goeladitya animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT capalashneena animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT sharmaprince animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT suricr animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT gandhisonu animmunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT mishrapriya immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT bangaivneet immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT tyagiroshika immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT munjaltanya immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT goeladitya immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT capalashneena immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT sharmaprince immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT suricr immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples
AT gandhisonu immunochromatographicdipstickasanalternateformonitoringofheroinmetabolitesinurinesamples