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Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces

Hybrid nanobiointerfaces were designed as an original contribution to the challenge of synthesizing nanostructured biomaterials integrating a set of cell fate-determining cues, originally provided to cells by the extracellular matrix (ECM). The produced biointerfaces consist of a stiff framework of...

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Autores principales: Lefèvre, Damien, Louvegny, Juliette, Naudin, Mathieu, Ferain, Etienne, Dupont-Gillain, Christine, Demoustier-Champagne, Sophie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081635/
https://www.ncbi.nlm.nih.gov/pubmed/35540120
http://dx.doi.org/10.1039/c8ra00325d
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author Lefèvre, Damien
Louvegny, Juliette
Naudin, Mathieu
Ferain, Etienne
Dupont-Gillain, Christine
Demoustier-Champagne, Sophie
author_facet Lefèvre, Damien
Louvegny, Juliette
Naudin, Mathieu
Ferain, Etienne
Dupont-Gillain, Christine
Demoustier-Champagne, Sophie
author_sort Lefèvre, Damien
collection PubMed
description Hybrid nanobiointerfaces were designed as an original contribution to the challenge of synthesizing nanostructured biomaterials integrating a set of cell fate-determining cues, originally provided to cells by the extracellular matrix (ECM). The produced biointerfaces consist of a stiff framework of intersected polypyrrole (PPy) nanotubes supporting a soft multilayer composed of ECM-derived biomacromolecules: collagen (Col) and hyaluronic acid (HA). PPy frameworks with highly tunable characteristics were synthesized through chemical oxidative polymerization of pyrrole monomers, templated within track-etched polycarbonate (PC) membranes featuring a network of intersected nanopores. PPy interfaces with a porosity of 80%, composed of nanotubes with an average diameter ranging from 40 to 300 nm, intersecting at an angle of 90°, were shown to be self-supported. These rigid PPy nanostructured interfaces were functionalized with a self-assembling (HA/Col) multilayer deposited via a layer-by-layer process. Biofunctionalized and unmodified PPy frameworks were both shown to promote sustained cell adhesion, therefore demonstrating the cytocompatibility of the engineered matrices. Such nanobiointerfaces, combining a mechanically-stable framework of tunable dimensions with a soft biopolymeric multilayer of highly versatile nature, pave the way towards cell-instructive biomaterials able to gather a wide range of cues guiding cell behavior. The developed self-supported structures could be used as a coating or as membranes bridging different tissues.
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spelling pubmed-90816352022-05-09 Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces Lefèvre, Damien Louvegny, Juliette Naudin, Mathieu Ferain, Etienne Dupont-Gillain, Christine Demoustier-Champagne, Sophie RSC Adv Chemistry Hybrid nanobiointerfaces were designed as an original contribution to the challenge of synthesizing nanostructured biomaterials integrating a set of cell fate-determining cues, originally provided to cells by the extracellular matrix (ECM). The produced biointerfaces consist of a stiff framework of intersected polypyrrole (PPy) nanotubes supporting a soft multilayer composed of ECM-derived biomacromolecules: collagen (Col) and hyaluronic acid (HA). PPy frameworks with highly tunable characteristics were synthesized through chemical oxidative polymerization of pyrrole monomers, templated within track-etched polycarbonate (PC) membranes featuring a network of intersected nanopores. PPy interfaces with a porosity of 80%, composed of nanotubes with an average diameter ranging from 40 to 300 nm, intersecting at an angle of 90°, were shown to be self-supported. These rigid PPy nanostructured interfaces were functionalized with a self-assembling (HA/Col) multilayer deposited via a layer-by-layer process. Biofunctionalized and unmodified PPy frameworks were both shown to promote sustained cell adhesion, therefore demonstrating the cytocompatibility of the engineered matrices. Such nanobiointerfaces, combining a mechanically-stable framework of tunable dimensions with a soft biopolymeric multilayer of highly versatile nature, pave the way towards cell-instructive biomaterials able to gather a wide range of cues guiding cell behavior. The developed self-supported structures could be used as a coating or as membranes bridging different tissues. The Royal Society of Chemistry 2018-06-22 /pmc/articles/PMC9081635/ /pubmed/35540120 http://dx.doi.org/10.1039/c8ra00325d Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Lefèvre, Damien
Louvegny, Juliette
Naudin, Mathieu
Ferain, Etienne
Dupont-Gillain, Christine
Demoustier-Champagne, Sophie
Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces
title Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces
title_full Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces
title_fullStr Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces
title_full_unstemmed Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces
title_short Biofunctionalized and self-supported polypyrrole frameworks as nanostructured ECM-like biointerfaces
title_sort biofunctionalized and self-supported polypyrrole frameworks as nanostructured ecm-like biointerfaces
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081635/
https://www.ncbi.nlm.nih.gov/pubmed/35540120
http://dx.doi.org/10.1039/c8ra00325d
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