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Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions

Plant and fruit surfaces are covered with cuticle wax and provide a protective barrier against biotic and abiotic stresses. Cuticle wax consists of very-long-chain fatty acids (VLCFAs) and their derivatives. β-Ketoacyl-CoA synthase (KCS) is a key enzyme in the synthesis of VLCFAs and provides a prec...

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Autores principales: Rizwan, Hafiz Muhammad, Shaozhong, Fang, Li, Xiaoting, Bilal Arshad, Muhammad, Yousef, Ahmed Fathy, Chenglong, Yang, Shi, Meng, Jaber, Mohammed Y. M., Anwar, Muhammad, Hu, Shuai-Ya, Yang, Qiang, Sun, Kaiwei, Ahmed, Mohamed A. A., Min, Zheng, Oelmüller, Ralf, Zhimin, Lin, Chen, Faxing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081883/
https://www.ncbi.nlm.nih.gov/pubmed/35548275
http://dx.doi.org/10.3389/fpls.2022.872263
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author Rizwan, Hafiz Muhammad
Shaozhong, Fang
Li, Xiaoting
Bilal Arshad, Muhammad
Yousef, Ahmed Fathy
Chenglong, Yang
Shi, Meng
Jaber, Mohammed Y. M.
Anwar, Muhammad
Hu, Shuai-Ya
Yang, Qiang
Sun, Kaiwei
Ahmed, Mohamed A. A.
Min, Zheng
Oelmüller, Ralf
Zhimin, Lin
Chen, Faxing
author_facet Rizwan, Hafiz Muhammad
Shaozhong, Fang
Li, Xiaoting
Bilal Arshad, Muhammad
Yousef, Ahmed Fathy
Chenglong, Yang
Shi, Meng
Jaber, Mohammed Y. M.
Anwar, Muhammad
Hu, Shuai-Ya
Yang, Qiang
Sun, Kaiwei
Ahmed, Mohamed A. A.
Min, Zheng
Oelmüller, Ralf
Zhimin, Lin
Chen, Faxing
author_sort Rizwan, Hafiz Muhammad
collection PubMed
description Plant and fruit surfaces are covered with cuticle wax and provide a protective barrier against biotic and abiotic stresses. Cuticle wax consists of very-long-chain fatty acids (VLCFAs) and their derivatives. β-Ketoacyl-CoA synthase (KCS) is a key enzyme in the synthesis of VLCFAs and provides a precursor for the synthesis of cuticle wax, but the KCS gene family was yet to be reported in the passion fruit (Passiflora edulis). In this study, thirty-two KCS genes were identified in the passion fruit genome and phylogenetically grouped as KCS1-like, FAE1-like, FDH-like, and CER6-like. Furthermore, thirty-one PeKCS genes were positioned on seven chromosomes, while one PeKCS was localized to the unassembled genomic scaffold. The cis-element analysis provides insight into the possible role of PeKCS genes in phytohormones and stress responses. Syntenic analysis revealed that gene duplication played a crucial role in the expansion of the PeKCS gene family and underwent a strong purifying selection. All PeKCS proteins shared similar 3D structures, and a protein–protein interaction network was predicted with known Arabidopsis proteins. There were twenty putative ped-miRNAs which were also predicted that belong to nine families targeting thirteen PeKCS genes. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation results were highly associated with fatty acid synthase and elongase activity, lipid metabolism, stress responses, and plant-pathogen interaction. The highly enriched transcription factors (TFs) including ERF, MYB, Dof, C2H2, TCP, LBD, NAC, and bHLH were predicted in PeKCS genes. qRT-PCR expression analysis revealed that most PeKCS genes were highly upregulated in leaves including PeKCS2, PeKCS4, PeKCS8, PeKCS13, and PeKCS9 but not in stem and roots tissues under drought stress conditions compared with controls. Notably, most PeKCS genes were upregulated at 9th dpi under Fusarium kyushuense biotic stress condition compared to controls. This study provides a basis for further understanding the functions of KCS genes, improving wax and VLCFA biosynthesis, and improvement of passion fruit resistance.
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spelling pubmed-90818832022-05-10 Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions Rizwan, Hafiz Muhammad Shaozhong, Fang Li, Xiaoting Bilal Arshad, Muhammad Yousef, Ahmed Fathy Chenglong, Yang Shi, Meng Jaber, Mohammed Y. M. Anwar, Muhammad Hu, Shuai-Ya Yang, Qiang Sun, Kaiwei Ahmed, Mohamed A. A. Min, Zheng Oelmüller, Ralf Zhimin, Lin Chen, Faxing Front Plant Sci Plant Science Plant and fruit surfaces are covered with cuticle wax and provide a protective barrier against biotic and abiotic stresses. Cuticle wax consists of very-long-chain fatty acids (VLCFAs) and their derivatives. β-Ketoacyl-CoA synthase (KCS) is a key enzyme in the synthesis of VLCFAs and provides a precursor for the synthesis of cuticle wax, but the KCS gene family was yet to be reported in the passion fruit (Passiflora edulis). In this study, thirty-two KCS genes were identified in the passion fruit genome and phylogenetically grouped as KCS1-like, FAE1-like, FDH-like, and CER6-like. Furthermore, thirty-one PeKCS genes were positioned on seven chromosomes, while one PeKCS was localized to the unassembled genomic scaffold. The cis-element analysis provides insight into the possible role of PeKCS genes in phytohormones and stress responses. Syntenic analysis revealed that gene duplication played a crucial role in the expansion of the PeKCS gene family and underwent a strong purifying selection. All PeKCS proteins shared similar 3D structures, and a protein–protein interaction network was predicted with known Arabidopsis proteins. There were twenty putative ped-miRNAs which were also predicted that belong to nine families targeting thirteen PeKCS genes. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) annotation results were highly associated with fatty acid synthase and elongase activity, lipid metabolism, stress responses, and plant-pathogen interaction. The highly enriched transcription factors (TFs) including ERF, MYB, Dof, C2H2, TCP, LBD, NAC, and bHLH were predicted in PeKCS genes. qRT-PCR expression analysis revealed that most PeKCS genes were highly upregulated in leaves including PeKCS2, PeKCS4, PeKCS8, PeKCS13, and PeKCS9 but not in stem and roots tissues under drought stress conditions compared with controls. Notably, most PeKCS genes were upregulated at 9th dpi under Fusarium kyushuense biotic stress condition compared to controls. This study provides a basis for further understanding the functions of KCS genes, improving wax and VLCFA biosynthesis, and improvement of passion fruit resistance. Frontiers Media S.A. 2022-04-25 /pmc/articles/PMC9081883/ /pubmed/35548275 http://dx.doi.org/10.3389/fpls.2022.872263 Text en Copyright © 2022 Rizwan, Shaozhong, Li, Bilal Arshad, Yousef, Chenglong, Shi, Jaber, Anwar, Hu, Yang, Sun, Ahmed, Min, Oelmüller, Zhimin and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Rizwan, Hafiz Muhammad
Shaozhong, Fang
Li, Xiaoting
Bilal Arshad, Muhammad
Yousef, Ahmed Fathy
Chenglong, Yang
Shi, Meng
Jaber, Mohammed Y. M.
Anwar, Muhammad
Hu, Shuai-Ya
Yang, Qiang
Sun, Kaiwei
Ahmed, Mohamed A. A.
Min, Zheng
Oelmüller, Ralf
Zhimin, Lin
Chen, Faxing
Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions
title Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions
title_full Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions
title_fullStr Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions
title_full_unstemmed Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions
title_short Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions
title_sort genome-wide identification and expression profiling of kcs gene family in passion fruit (passiflora edulis) under fusarium kyushuense and drought stress conditions
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9081883/
https://www.ncbi.nlm.nih.gov/pubmed/35548275
http://dx.doi.org/10.3389/fpls.2022.872263
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